The morphologies of Cu3HHTP2 and MOF-derived CuO/C were observed by field emission scanning electron microscopy (FE-SEM, Hitachi SU8020). The ultrahigh-resolution transmission electron microscopy (TEM) was performed using a ThermoFisher Themis Z TEM instrument. For the preparation of TEM samples, the focused ion beam (FIB, Helios NanoLab G3 UC) system was used. Note that Cu3HHTP2 was passivated by aluminum and amorphous carbon for energy-dispersive X-ray spectroscopy (EDS) and imaging analysis, respectively. High-resolution Raman spectra and mapping images were obtained by employing a Renishaw inVia Qontor system using 532 nm laser excitation with a laser power of 5 mW. A Nicolet Continuum infrared microscope (Thermo Scientific) was used to collect the Fourier transform infrared (FT-IR) spectra. X-ray diffraction (XRD) patterns of Cu3HHTP2 MOFs were recorded on an Empyrean X-ray diffractometer (Malvern Panalytical) with Cu Kα radiation (λ = 1.54056 Å). X-ray photoelectron spectroscopy (XPS) was performed using an ESCALAB 250Xi system (Thermo Scientific). The time of flight secondary ion mass spectrometry (ToF–SIMS) was conducted by TOF–SIMS 5–100 (Ion-tof) instrument. A primary beam with bismuth (Bi) was applied for spectrometry (30 keV, 0.9 pA). These analyzes were performed at the DGIST Center for Core Research Facilities (CCRF).
Nicolet continuum infrared microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nicolet Continuum infrared microscope is a versatile analytical instrument designed for microscopic analysis of samples. It provides high-resolution infrared imaging and spectroscopy capabilities, allowing users to investigate the chemical composition and molecular structure of microscopic samples.
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Lab products found in correlation
Empyrean x ray diffractometer, by Malvern Panalytical (2 mentions)
Escalab 250xi system, by Thermo Fisher Scientific (2 mentions)
Tof sims 5 100, by ION-TOF (1 mentions)
Su8020, by Hitachi (1 mentions)
Hemocytometer, by Solarbio (1 mentions)
Diff quick stain kit, by Solarbio (1 mentions)
Hf 3300, by Hitachi (1 mentions)
S 4800, by Hitachi (1 mentions)
Su8230, by Hitachi (1 mentions)
10 protocols using nicolet continuum infrared microscope
1
Morphological Characterization of Cu3HHTP2 and CuO/C
2
Characterization of Functionalized 3D Scaffolds
3
FTIR Spectroscopic Analysis of Leaf Samples
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Leaf samples were dried for 72 h at 60 °C in an oven. About 1 mg of the dried tissue was mixed with 200 mg of oven-dried potassium bromide (KBr) (Sigma, France) to make a transparent disk using the finely ground mixture of a leaf sample with the aid of a hydraulic press. KBr was used as the blank during the FTIR procedure. The FTIR micro-spectroscopic imaging system used was “Nicolet Continuum Infrared Microscope” (Thermo scientific, USA), using the omnic series software. Each sample was scanned 16× and collected in absorbance mode in the 4000–800 cm−1 region at 4 cm−1 spectral resolution. Peaks’ functional groups were assigned according to Mafa et al. [26 (link),62 (link)].
4
Characterization of Graphene Oxide and Functionalized Derivatives
5
Characterization of Synthesized Materials
6
Nasal Lavage Fluid Analysis in Mice
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Blood was harvested via cardiac puncture from the sacrificed mice, and serum was collected by centrifugation. The trachea was partially resected, and then an 18-gauge catheter was inserted into the trachea towards upper airway into the nasopharynx. Next, the nasal passage was perfused with 1 mL pre-cooled phosphate buffer saline (PBS, Gibco, California, US). Then, nasal lavage fluid (NALF) was collected and centrifuged to obtain the supernatants for cytokines measurements. The cells were suspended in PBS and the total number of cells was counted with a hemocytometer (Solarbio, Beijing, China). An amount of 150 μL NALF was centrifuged onto glass slides using a Cytopro Cytocentrifuge Series 2 cytospin device (ELITech Group, Puteaux, France) at 1000 rmp for 10 min at 4°C. A Diff-Quick stain kit (Solarbio, Beijing, China) was used for cell staining in accordance with the manufacturer’s protocol. Finally, a Nicolet Continuum Infrared Microscope (Thermo Scientific, California, USA) was used for observation.
7
Synchrotron FTIR Microspectroscopy of Tissues
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Synchrotron FTIR microspectroscopy (SR-μFTIR) investigation was conducted at the BM02-IR beamline61 (link),62 (link) of SESAME (Synchrotron-light for Experimental Science and Applications in the Middle East), Jordan. Two to three sections were measured for each tissue type. Samples were deposited on CaF2 IR windows and an area of interest was selected to be chemically mapped and constructed with an aperture of 30 × 30 μm2 for each tissue section using Atlμs© software (Thermo Fisher Scientific©, USA). Maps were collected in transmission mode using Thermo Nicolet 8700 FTIR spectrometer, coupled with a Nicolet Continuum infrared microscope (Thermo Fisher Scientific©, USA). The microscope is equipped with the single-point detector, Mercury Cadmium Telluride (MCT-A), a 10× visible objective, and a 15× [NA (Numerical Aperture) = 0.58] IR/visible Schwarzschild objective matched with a 15× condenser. Samples were mounted on a Prior Scan© motorized sample stage and spectra were acquired with 256 co-added scans at a spectral resolution of 4 cm−1, levels Zero filling 1, and Happ-Genzel apodization window in the mid-IR range between 650 and 4000 cm−1 with Thermo OMNIC© and Atlµs© software package. Background spectra were collected to assess the spectral contribution. The background signal was measured from a part of the CaF2 substrate without biological tissue.
8
Characterization of LIG@Cu3HHTP2 Materials
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The morphologies of LIG@Cu3HHTP2 were observed by field emission scanning electron microscopy (FE-SEM, Hitachi S-4800 (Fig. 2a, b ), and SU8230 (inset of Fig. 2b )). TEM was performed using a Hitachi HF-3300 instrument. For the preparation of TEM samples, LIG@Cu3HHTP2 was peeled off PI and transferred onto a lacey carbon-supported nickel TEM grid. High-resolution Raman spectra and mapping images were obtained by employing a Renishaw inVia Qontor system using 532 nm laser excitation with a laser power of 5 mW. A Nicolet Continuum infrared microscope (Thermo Scientific) was used to collect the FT-IR spectra. XRD patterns of Cu3HHTP2 MOFs and LIG were recorded on an Empyrean X-ray diffractometer (Malvern Panalytical) with Cu Kα radiation (λ = 1.54056 Å). XPS and UPS were performed using an ESCALAB 250Xi system (Thermo Scientific).
9
IR Microspectroscopy Analysis of Thin Films and NPs
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IR microspectroscopy measurements were recorded on a Nicolet Continuum infrared microscope (Thermo Fisher Scientific) equipped with a liquid nitrogen cooled MCT detector, with a ×32 objective. Spectra were recorded in transmission mode covering the 4000 to 650 cm -1 energy range. Spectra were acquired by averaging 128 scans and were recorded at 2 cm -1 spectral resolution. The aperture dimension on the sample was set to 50 × 50 or 75 × 75 µm, depending on the local homogeneity of the area. Films and NPs were analysed as bulk. For a reliable quantitative analysis, the thickness of the analyzed samples was carefully optimized and controlled using a diamond compression cell. Smooth and flat samples in the μm thickness range were obtained.
For each sample, at least five independent areas were analysed. Before each analysis, a background spectrum was collected in a clean region of the diamond substrate without sample. The transmittance spectrum was obtained by dividing the sample spectrum by this background. The spectra were extracted in transmittance, and samples thin film interference fringes were baseline-corrected through a spline function generated in the software Origin Pro 8. Then spectra were converted to absorbance.
For each sample, at least five independent areas were analysed. Before each analysis, a background spectrum was collected in a clean region of the diamond substrate without sample. The transmittance spectrum was obtained by dividing the sample spectrum by this background. The spectra were extracted in transmittance, and samples thin film interference fringes were baseline-corrected through a spline function generated in the software Origin Pro 8. Then spectra were converted to absorbance.
10
Infrared Fingermark Analysis via ATR
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Analyses were carried out on a Thermo Scientific Nicolet Continuum infrared microscope equipped with a conventional liquid-nitrogen-cooled mercury cadmium telluride (MCT) detector. During ATR analysis, the tip of the germanium crystal touched the fingermarks in order to gather spectral information from the surface layer of the samples only.
128 scans were taken per sample with 4 cm -1 resolution over a range of 650-4000 cm -1 (1738 variables). All the spectra were collected with the OMNIC 3.2 software and each was saved in *.SPA and *.CSV formats before being further processed. Before all sample analyses, background spectra were acquired under the same conditions as the sample spectra from a clean (fingermark-free) area of the substrate. Furthermore, the sensitivity of the infrared microscope was monitored by analysing a controlled amount of cholesterol powder each time before each set of sample analyses. This allowed the establishment of a set of control intensities for specific vibrational bands (e.g., ν(OH), ν(CH) aliphatic and ν(CH) aromatic ). This procedure ensured the quality and comparability of the acquired sample spectra.
128 scans were taken per sample with 4 cm -1 resolution over a range of 650-4000 cm -1 (1738 variables). All the spectra were collected with the OMNIC 3.2 software and each was saved in *.SPA and *.CSV formats before being further processed. Before all sample analyses, background spectra were acquired under the same conditions as the sample spectra from a clean (fingermark-free) area of the substrate. Furthermore, the sensitivity of the infrared microscope was monitored by analysing a controlled amount of cholesterol powder each time before each set of sample analyses. This allowed the establishment of a set of control intensities for specific vibrational bands (e.g., ν(OH), ν(CH) aliphatic and ν(CH) aromatic ). This procedure ensured the quality and comparability of the acquired sample spectra.
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