Surface-spread chromosomes of mouse spermatocytes were prepared from 18 days postpartum male mice using the drying-down technique as previously described33 (link). Immuno-staining was performed as previously described33 (link) using the following primary and secondary antibodies: goat anti-SYCP3 (Santa Cruz Biotechnology, sc-20845; 1:200), rabbit anti-MSH4 (a gift from Dr. Paula Cohen; 1:200), mouse monoclonal anti-MLH1 (4C9C7) (Cell Signaling Technology, 3515S; 1:30), AMCA anti-goat (Jackson ImmunoResearch, 705–155-147; 1:50), Cy3 anti-rabbit (Jackson ImmunoResearch, 711–165-152; 1:100), and AlexaFluor647 anti-mouse (Jackson ImmunoResearch, 715–605-151; 1:100).
Goat anti sycp3
Manufactured by Santa Cruz Biotechnology
Goat anti-SYCP3 is a primary antibody that recognizes the SYCP3 protein. SYCP3 is a key structural component of the synaptonemal complex, which is essential for proper chromosome organization and segregation during meiosis. This antibody can be used for the detection of SYCP3 in various applications, such as immunohistochemistry and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit cy3, by Jackson ImmunoResearch (2 mentions)
Alexa fluor 647 anti mouse, by Jackson ImmunoResearch (2 mentions)
Mouse monoclonal anti mlh1 4c9c7, by Cell Signaling Technology (2 mentions)
Amca anti goat, by Jackson ImmunoResearch (2 mentions)
Rabbit anti dmc1, by Santa Cruz Biotechnology (1 mentions)
Donkey anti rabbit 488, by Thermo Fisher Scientific (1 mentions)
Donkey anti mouse alexa fluor 488, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)
Mouse anti mlh1, by BD (1 mentions)
4 protocols using goat anti sycp3
1
Immunostaining of Mouse Spermatocyte Chromosomes
2
Immunolabeling of Meiotic Chromosome Spreads
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Spermatocytes for surface spreading were prepared from testes of ~2-month-old mice using established methods80 (link). The following primary antibodies were used in dilution buffer (0.2% BSA, 0.2% fish gelatin, 0.05% Triton X-100, 1× PBS), with incubation overnight at 4°C: goat anti-SYCP3 (Santa Cruz Biotechnology Cat# sc-20845; 1:200), rabbit anti-RAD51 (Calbiochem Cat# PC130; 1:200), rabbit anti-DMC1 (Santa Cruz Biotechnology Cat# sc-22768; 1:200). This was followed by incubation with the following secondary antibodies at 1:500 dilution for 1 h at 37°C: 488 donkey anti-rabbit (Life Technologies Cat#A21206), donkey 594 anti-goat (Invitrogen Cat# A11058). Coverslips were mounted with ProLong Gold antifade reagent with DAPI. Immunolabeled chromosome spread nuclei were imaged on a Deltavision microscope using ×100 oil-immersion objective. Only foci colocalizing with the chromosome axis were counted.
3
Immunostaining of Meiotic Cell Spreads
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Meiotic cell spreads were prepared using the method of Peters et al. (1997) (link) with minor modifications as described (Dumont et al. 2015 (link)). Spermatocyte cell spreads were immunostained according to a protocol adapted from Anderson et al. (1999) (link) and Koehler et al. (2002) (link) as previously described (Dumont et al. 2015 (link)). The primary antibodies used were as follows: mouse anti-MLH1 (1:100 dilution; BD Biosciences), goat anti-SYCP3 (1:100 dilution; Santa Cruz Biotechnology), rabbit anti-phospho-histone H2A.XpSer139 (1:1000 dilution; Thermo Scientific), human anti-centromere (1:100 dilution; Antibodies Incorporated), and rabbit anti-SYCP1 (1:100 dilution; Abcam). The following secondary antibodies were used at 1:200 concentration: donkey anti-goat Rhodamine Red-X, donkey anti-rabbit Alexa Fluor 488, donkey anti-human Aminomethylcoumarin Acetate, and donkey anti-mouse Alexa Fluor 488 (Jackson ImmunoResearch).
4
Immunostaining of Mouse Spermatocyte Chromosomes
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Surface-spread chromosomes of mouse spermatocytes were prepared from 18 days postpartum male mice using the drying-down technique as previously described33 (link). Immuno-staining was performed as previously described33 (link) using the following primary and secondary antibodies: goat anti-SYCP3 (Santa Cruz Biotechnology, sc-20845; 1:200), rabbit anti-MSH4 (a gift from Dr. Paula Cohen; 1:200), mouse monoclonal anti-MLH1 (4C9C7) (Cell Signaling Technology, 3515S; 1:30), AMCA anti-goat (Jackson ImmunoResearch, 705–155-147; 1:50), Cy3 anti-rabbit (Jackson ImmunoResearch, 711–165-152; 1:100), and AlexaFluor647 anti-mouse (Jackson ImmunoResearch, 715–605-151; 1:100).
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