Whole cell lysates were prepared, resolved and blotted as described previously [20 (link)]. CXCR4 expression was detected using an anti-human CXCR4 antibody (Abcam) at 1:1000. Expression of CXCR1 and CXCR2 were detected as previously described [30 (link)]. Membranes were re-probed with GAPDH antibody to ensure equal loading (Biogenesis, Dorset, UK).
Anti human cxcr4 antibody
Manufactured by Abcam
Sourced in United States
Anti-human CXCR4 antibody is a laboratory reagent that can be used to detect and study the CXCR4 protein, which is a chemokine receptor that plays a role in various cellular processes. The antibody can be utilized in techniques such as flow cytometry, immunohistochemistry, and western blotting to identify and analyze the CXCR4 protein.
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Lab products found in correlation
3 protocols using anti human cxcr4 antibody
1
Western Blot Analysis of CXCR Expression
2
CXCR4 Signaling Pathway Regulation
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Human recombinant chemokine SDF-1α/CXCL12 was obtained from R&D Systems Inc. (Minneapolis, MN, USA). Anti-human CXCR4 antibody was purchased from Abcam (Cambridge, MA, USA). CXCR4 antagonist, AMD3100, was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). AG490 was purchased from DuPont Merck (Hangzhou, China). STAT3 and phospho-STAT3 (Serine 727) antibodies were purchased from Cell Signaling Technology (Boston, MA, USA).
3
Immunohistochemical Detection of Fibrocytes
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The lungs were fixed in 10% buffered formalin and embedded in paraffin. Sections (3 to 4 μm) were stained with hematoxylin and eosin. Paraffin-embedded lung sections were stained to detect fibrocytes using the following antibodies : biotin-conjugated anti-CD45 antibody (Cell Signaling Tech, Danvers, MA, USA), secondary antibody : streptavidin-FITC (eBioscience, Ireland, UK) ; anti-collagen-type 1 antibody (Abcam ; Cambridge, UK) and anti-FSP-1 antibody (Thermo Fisher scientific Inc.), and secondary antibodies conjugated with Alexa Flour 594 or 647 (Thermo Fisher scientific Inc.), respectively.
For triple staining to detect PDGF-BB in fibrocytes, the following antibodies were used : anti-human FSP-1 antibody, (Thermo Fisher scientific Inc.), anti-human CXCR4 antibody (Abcam) and anti-human PDGF-BB antibody (Novus biological, Litteleton, CO, USA), and the fluorescent secondary antibodies conjugated with Alexa Flour 488, 594 or 647 (Thermo Fisher scientific Inc.), respectively. To detect proliferating cells, the section was first stained with anti-Ki-67 antibody (Agilent Technologies) followed by the secondary antibody conjugated with Alexa Flour 594 were used. Images were taken using a confocal laser scanning microscope, A1 system (Nikon, Tokyo, Japan).
For triple staining to detect PDGF-BB in fibrocytes, the following antibodies were used : anti-human FSP-1 antibody, (Thermo Fisher scientific Inc.), anti-human CXCR4 antibody (Abcam) and anti-human PDGF-BB antibody (Novus biological, Litteleton, CO, USA), and the fluorescent secondary antibodies conjugated with Alexa Flour 488, 594 or 647 (Thermo Fisher scientific Inc.), respectively. To detect proliferating cells, the section was first stained with anti-Ki-67 antibody (Agilent Technologies) followed by the secondary antibody conjugated with Alexa Flour 594 were used. Images were taken using a confocal laser scanning microscope, A1 system (Nikon, Tokyo, Japan).
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