Protx 2

Manufactured by Peptide Institute

Sourced in Japan

ProTx-II is a lab equipment product that functions as a peptide. It is a naturally occurring compound derived from the venom of the Peruvian Green Velvet Tarantula.

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Lab products found in correlation

Trypsin, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Fujifilm (1 mentions) Protx 1, by Peptide Institute (1 mentions) Gsmtx 4, by Peptide Institute (1 mentions) Gtx1 15, by Alomone (1 mentions) Tris hcl, by Fujifilm (1 mentions) α chymotrypsin, by Merck Group (1 mentions) Glycine, by Fujifilm (1 mentions) Elastase, by Fujifilm (1 mentions) Pepsin, by Fujifilm (1 mentions) Menthol, by Merck Group (1 mentions) Gadolinium chloride, by Merck Group (1 mentions) Resiniferatoxin, by LC Laboratories (1 mentions)

4 protocols using protx 2

Peptide Preparation and Enzymatic Digestion

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Human prolactin-releasing peptide (hPRP), ProTx-I, ProTx-II, and GsMTx-4 were purchased from Peptide Institute, Inc. (Osaka, Japan). GTx1-15 was obtained from Alomone Labs (Jerusalem, Israel). Pepsin, elastase, glycine, HCl, and Tris-HCl were purchased from Wako (Osaka, Japan). Trypsin and α-chymoTrypsin were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Tokyo Kasei (Tokyo, Japan), respectively. All enzymes and peptides were dissolved in distilled water to make 100 ng/μL solutions. glycine was dissolved to make 1 M solution and adjusted to pH2.0 with 5 M HCl and 1 M Tris-HCl (pH8.0) was diluted to 500 mM.

Kikuchi K., Sugiura M, & Kimura T. (2015). High Proteolytic Resistance of Spider-Derived Inhibitor Cystine Knots. International Journal of Peptides, 2015, 537508.

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Investigating Neuropathic Pain Pathways

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We purchased capsaicin and complete Freund’s adjuvant (CFA), menthol, and gadolinium chloride from Sigma-Aldrich, siRNA from Origene, resiniferatoxin from LC Laboratories, and ProTx-II from Peptide institute, Inc (Osaka, Japan).

Han Q., Kim Y.H., Wang X., Liu D., Zhang Z.J., Bey A.L., Lay M., Chang W., Berta T., Zhang Y., Jiang Y.H, & Ji R.R. (2016). SHANK3 deficiency impairs heat hyperalgesia and TRPV1 signaling in primary sensory neurons. Neuron, 92(6), 1279-1293.

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Purification and Storage of Neurotoxins

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ProTx-I and ProTx-II were acquired from Peptides International (USA), PaurTx3 from Alomone Labs (Israel). AaHII from Androctonus australis hector venom was purified as described (Martin et al., 1987 (link)). Toxins were kept at -20°C and aliquots were dissolved in appropriate solutions containing 0.1% BSA.

Das S., Gilchrist J., Bosmans F, & Van Petegem F. (2016). Binary architecture of the Nav1.2-β2 signaling complex. eLife, 5, e10960.

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Selective Interaction of Tarantula Venom Peptide with Nav1.7

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Tarantula venom peptide ProTx-II, which selectively interacts with Nav1.7 sodium channel, was purchased from Peptide Institute Inc. (Ibaraki, Osaka, Japan). (5-[4-chloro-phenyl]-furan2-carboxylic acid [3,5-dimethoxy-phenyl]-amide (A803467), a selective Nav1.8 sodium channel blocker, and STZ were purchased from Sigma-Aldrich Co. ProTx-II was dissolved in saline. A803467 was dissolved in 30% dimethyl sulfoxide (DMSO) in saline. For vehicle-only control groups, equal volumes of DMSO or saline were injected.

Tanaka K.I., Sekino S., Ikegami M., Ikeda H, & Kamei J. (2015). Antihyperalgesic effects of ProTx-II, a Nav1.7 antagonist, and A803467, a Nav1.8 antagonist, in diabetic mice. Journal of Experimental Pharmacology, 7, 11-16.

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