Anti pd l1 10f 9g2

The combination regimen used for the MC38 colon adenocarcinoma mouse model was capecitabine (Actavis Group PTC ehf) administered per os 5 days a week at a dose of 250mg/kg and oxaliplatin (Mylan), injected i.p. once a week at a dose of 5 mg/kg + anti-PD-1 (RMP1-14, BioXCell), injected i.p. once a week at a dose of 12.5 mg/kg. For the metastatic 4T1 breast cancer mouse model, the combination was cyclophosphamide (Baxter) injected i.p. once a week at a dose of 100 mg/kg + doxorubicin (Accord Healthcare) injected i.p. once a week at a dose of 2 mg/kg + anti-PD-1 (RMP1-14, BioXCell) or anti-PD-L1 (10F.9G2, BioXCell), injected i.p. once a week at a dose of 12.5 mg/kg. For bladder cancer mouse models, the MVAC regimen consisted in a combination of methotrexate (Mylan) injected i.p. once a week at a dose of 1 mg/kg + vinblastine (EG Labo) injected i.p. once a week at a dose of 0.1 mg/kg + doxorubicin (Accord Healthcare) injected i.p. once a week at a dose of 1 mg/kg + cisplatine (Mylan) injected i.p. once a week at a dose of 1 mg/kg, + anti-PD-L1 (10F.9G2, BioXCell) injected i.p. once a week at a dose of 12.5 mg/kg and + anti-PD-1 (RMP1-14, BioXCell) injected i.p. once a week at a dose of 12.5 mg/kg for MB49 mouse model.

Murine melanoma B16F10, 293T, and EL4 cells were obtained from the American Type Culture Collection (Manassas, VA). All cell lines were cultured as recommended by the provider. Synthetic peptides representing the CD8 T-cell epitopes Ova₂₅₇ (SIINFEKL) and Trp2₁₈₀ (SVYDEFVWL) were purchased at >80% purity from A&A Labs (San Diego, CA). Monoclonal anti-mouse CD40 (FGK45.5) and anti-PD-L1 (10F.9G2) were purchased from BioXCell (West Lebanon, NH). Fluorescence-conjugated antibodies for flow cytometry were from eBioscience (San Diego, CA).