The normal human liver cell line (L02) was purchased from The Cell Collection Center of Wuhan University. The Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Chemical Technology Co., Ltd. GLY was purchased from Selleck Chemicals. The lactate dehydrogenase (LDH), malondialdehyde (MDA), glutathione (GSH) and ROS kits were purchased from Beyotime Institute of Biotechnology. The primary antibodies against glutathione peroxidase 4 (GPX4, cat. no. sc-166570) was purchased from Santa Cruz Biotechnology, Inc. GAPDH (cat. no. 10494-1-AP) were purchased from ProteinTech Group, Inc. The primary antibodies against high mobility group box 1 (HMGB1, cat. no. 3935), nuclear factor E2-related factor 2 (Nrf2, cat. no. 12721) and homooxygenase-1 (HO-1, cat. no. 43966) were purchased from Cell Signaling Technology, Inc. The goat anti-rabbit IRDye fluorescent secondary antibody IRDye800CW (cat. no. 926-32211) was purchased from LI-COR Biosciences. Cy3 (cat. no. BA1031 and BA1032) and FITC (cat. no. BA1101 and BA1105) fluorescently labeled rabbit anti-goat secondary antibodies were purchased from Wuhan Boster Biological Technology, Ltd. The iron ion detection kit was purchased from Abcam. D-GalN, LPS and TNF-α were purchased from Sigma-Aldrich; Merck KGaA.
Iron ion detection kit
Manufactured by Abcam
The Iron ion detection kit is a laboratory tool designed to detect and quantify the presence of iron ions in a sample. It provides a reliable and efficient method for measuring iron levels across a range of research and analytical applications.
Automatically generated - may contain errors
3 protocols using iron ion detection kit
1
Evaluating Liver Cell Antioxidant Defenses
2
Quantification of Total Iron in HUVEC Lysate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total iron content in HUVEC lysate was determined using an iron ion detection kit (Abcam). HUVECs or HUVECs treated with Exos were homogenized with normal saline and PBS and centrifuged at 16,000 × g for 10 min for removal of insoluble matter. Then, 50 µL samples were added with 5 µL iron reductant to obtain total iron content (Fe 3+ and Fe 2+). Subsequently, the samples were added with 100 μL iron probe solution and incubated at 25°C in the dark conditions for 60 min. The absorbance at 593 nm was detected using the spectrophotometry method.
3
Lipid Peroxidation and Antioxidant Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atorvastatin, cerivastatin, fluvastatin, and simvastatin, were purchased from Gödecke/Parke-Davis (Freiburg, Germany). Ferrostatin-1 (#S7243) was from Selleck Chemical (Houston, TX, USA). GGPP was obtained from GlpBio (Montclair, NJ, USA). The iron ion detection kit was purchased from Abcam. The malondialdehyde (MDA), and glutathione (GSH) kits are from Beyotime (Shanghai, China). ROS kit was purchased from bjbalb Inc. (Beijing, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!