Potassium hexacyanoferrate (III), methanol, sodium nitrite, and hydrochloric acid were purchased from Chempur (Poland). Phosphate-buffered saline (PBS), 4-aminobenzoic acid (4-ABA), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), Tris-buffered saline (TBS), IPTG, HIS-Select® Nickel Affinity Gel, Tris-HCl, Glycerol, 4-aminothiphenol (4-ATP), glutaraldehyde (GA), bovine serum albumin (BSA), ethanol, demineralized/ultrapure water (ddH2O), hydrochloric acid (37%), sodium nitrite and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Aldrich (Germany). Codon optimization for E. coli, ALICator Ligation Independent Cloning, B-PER Complete Bacterial Protein Extraction Reagent, Gene Art Gene Synthesis, Slide-A-Lyzer Dialysis Cassettes, Wedge Well Gel, Super Signal West Pico Plus Chemiluminescent Substrate, and 1-Step Turbo TMB-ELISA were purchased from Thermo Fisher Scientific (USA). pFastBac1 was purchased from Invitrogen (USA). 15 cm Chromatography column, Trans-Blot Turbo RTA Transfer Kit were purchased from Biorad (USA). Protein A-agarose affinity matrix was purchased from Roche (Swiss). Instant Blue was purchased from Expedeon (United Kingdom). Secondary goat anti-rabbit HRP antibodies were purchased from Jackson ImmunoResearch (United Kingdom). 96-well ELISA plate was purchased from Greiner Microlon (Germany).
Sodium nitrite
Manufactured by Chempur
Sourced in Poland
Sodium nitrite is a chemical compound with the formula NaNO2. It is a white to slightly yellowish crystalline solid that is commonly used as a food preservative and in the production of various industrial chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Chempur (5 mentions)
Folin ciocalteu reagent, by Merck Group (4 mentions)
Potassium hexacyanoferrate 3, by Chempur (3 mentions)
Phosphate buffered saline (pbs), by Merck Group (3 mentions)
Aluminum chloride hexahydrate, by Chempur (3 mentions)
Potassium persulfate, by Chempur (3 mentions)
Sodium acetate anhydrous, by Chempur (3 mentions)
Sodium carbonate anhydrous, by Chempur (3 mentions)
Rutin trihydrate, by Carl Roth (3 mentions)
Sodium hydroxide (naoh), by Chempur (3 mentions)
Methanol, by Chempur (3 mentions)
Iron 2 sulfate heptahydrate, by Merck Group (3 mentions)
Copper 2 chloride dihydrate, by Chempur (3 mentions)
Iron 3 chloride hexahydrate, by Chempur (3 mentions)
Neocuproine, by J&K Scientific (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Trolox, by Merck Group (3 mentions)
Gallic acid, by Merck Group (3 mentions)
Acetic acid, by Chempur (3 mentions)
Tris buffered saline (tbs), by Merck Group (2 mentions)
7 protocols using sodium nitrite
1
Recombinant Protein Expression and Purification
2
Spectrophotometric Analysis of Antioxidant Compounds
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Acetic acid (99.5%), aluminum chloride hexahydrate, copper(II) chloride dihydrate, disodium hydrogen phosphate dihydrate, 96% ethanol, 36% hydrochloric acid, iron(III) chloride hexahydrate, methanol, phosphoric acid, potassium persulfate, potassium dihydrogen phosphate, potassium hexacyanoferrate(III), sodium acetate anhydrous, sodium carbonate anhydrous, sodium hydroxide, sodium nitrite, and trichloroAcetic acid were purchased from Chempur, Poland. Neocuproine was delivered by J&K Scientific, Germany. Folin–Ciocalteu reagent, acetonitrile, iron(II) sulfate heptahydrate, gallic acid were supplied by Merck, Germany, whereas rutin trihydrate by Roth, Germany. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), caffeine were purchased from Sigma-Aldrich, USA. All the chemicals were of analytical grade.
3
Antibody Production and Characterization
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Phosphate-buffered saline (PBS), 4-aminobenzoic acid, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), and PCR primers were purchased from Sigma-Aldrich (Germany). Potassium hexacyanoferrate (III), hexaammineruthenium (III), sodium nitrite, hydrochloric acid, and dipotassium phosphate monopotassium phosphate were purchased from ChemPur (Poland). An ExtractMe DNA Bacteria kit was purchased from Blirt (Poland), and a SensiFAST SYBR No-ROX kit was purchased from Bioline (UK). Phosphate buffer with different pH (5.68, 7.01, 7.99) was prepared by mixing the K2HPO4 and KH2PO4 in different ratios. All reagents were used without further purification. Aqueous solutions were prepared with the usage of double-distilled sterile water (ddH2O).
All biomaterials utilised in this manuscript are accurately described elsewhere [30 (link)]. Herein, we present them briefly. The monoclonal mouse antibodies were produced by the Nanobioengineering Laboratory at the Polish Center for Technology Development Ltd. through procedures described elsewhere [31 ], with slight changes. Recombinant protein D was isolated at the Institute of Biotechnology and Molecular Medicine following the standard procedures. The efficiency and quality of antibody production were verified with the ELISA test (data are not shown).
All biomaterials utilised in this manuscript are accurately described elsewhere [30 (link)]. Herein, we present them briefly. The monoclonal mouse antibodies were produced by the Nanobioengineering Laboratory at the Polish Center for Technology Development Ltd. through procedures described elsewhere [31 ], with slight changes. Recombinant protein D was isolated at the Institute of Biotechnology and Molecular Medicine following the standard procedures. The efficiency and quality of antibody production were verified with the ELISA test (data are not shown).
4
Antioxidant Capacity Assays Protocol
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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
5
Functionalized Diamond Nanoparticles for Antibody Detection
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Monoclonal antibodies (developed in mice) against Streptococcus agalactiae were obtained from ThermoFisher Scientific (Warsaw, Poland). The ND powder (monocrystalline Diamond Podwer (0–0.03 micron were obtained from Pureon (Lengwil, Switzerland). Potassium hexacyanoferrate (III), methanol, sodium nitrite and hydrochloric acid were purchased from Chempur (Piekary Śląskie, Poland). Phosphate-buffered saline (PBS), 4-aminobenzoic acid (4-ABA), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), Tris-buffered saline (TBS) and sodium azide (NaN3) were obtained from Sigma–Aldrich (Poznan, Poland). Alumina slurries of 0.3 m were purchased from Buehler (Lake Bluff, IL, USA). Sulphuric acid, potassium hydroxide, hydrogen peroxide, ethanol and methanol were supplied by Pol-Aura (Warsaw, Poland). The potato starch (PS) was purchased at a local supermarket from Grula® Trzemeszno, Poland).
6
Antioxidant Potential of Phenolic Compounds
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Methanol was obtained from Sigma-Aldrich, St. Quentin, France. Triton X-100 was purchased from PanReac AppliChem ITW Reagent, Barcelona, Spain. Aluminum chloride anhydrous (pure for analysis) and sodium nitrite (pure for analysis) were purchased from Chempur, Piekary Śląskie, Poland. Caffeic acid ≥ 98% and rutin ≥ 97% were obtained from Acros Organics, Berlin, Germany. Folin–Ciocalteu reagent, sodium carbonate, and hydrochloric acid were obtained from Sigma-Aldrich, Schnelldorf, Germany. Ethyl acetate and pyridine were purchased from VWR International S.A.S, Briare, France. Magnesium sulphate was obtained from VWR International, Leuven, Belgium. Resveratrol (5-[(E)-2-(4-hydroxyphenyl)vinyl]benzene-1,3-diol) was purchased from Apollo Scientific, Bredbury, UK. BSA 95–100% (N,O-bis-trimethylsilyl-acetamide) was obtained from Macherey-Nagel, Dueren, Germany. Sodium hydroxide was purchased from Carl Roth, Karlsruhe, Germany. DPPH (1,1-Diphenyl-2-picrylhydrazyl free radical) was obtained from TCI EUROPE, Zwijndrecht, Belgium. For all dilutions and extractions, bidistilled water was obtained using the distillation apparatus from Thermo Scientific (Waltham, MA, USA).
7
Antioxidant Capacity Assays Protocol
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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
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