i-Particles® (PLA nanoparticles) were purchased from Adjuvatis (Lyon, France). Lipids (1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)) were purchased from Avanti Polar (Alabaster, AL, USA). LAH4-L1 peptide (KKALLAHALHLLAL-LALHLAHALKKA) was purchased from GenScript (Piscataway, NJ, USA). Absolute anhydrous ethanol was purchased from Carlo Erba Reagents (Peypin, France), sterile pyrogen-free bidistilled water OTEC® was purchased from Aguettant (Lyon, France), and nuclease-free water was purchased from Ambion (Thermo Fisher Scientific, Waltham, MA USA). Furthermore, 1× and 10× DPBS (Dulbecco’s phosphate-buffered saline, pH 7.4), culture medium (RPMI/glutamax and DMEM), fetal bovine serum (FBS), N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES), and β-mercaptoethanol were all purchased from Gibco (Dublin, Ireland). Lipofectamine 2000TM transfection reagent was purchased from Invitrogen™ via Thermo Scientific™ (Waltham, MA, USA).
Ethanol absolute anhydrous
Manufactured by Carlo Erba
Sourced in France, Italy
Ethanol absolute anhydrous is a pure, anhydrous form of ethyl alcohol. It is a clear, colorless liquid with a characteristic odor. The product is suitable for various laboratory and analytical applications where a high-purity alcohol is required.
Automatically generated - may contain errors
Lab products found in correlation
Folin ciocalteu reagent, by Merck Group (6 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Glyceryl tributyrate, by Merck Group (4 mentions)
Soy l α phosphatidylcholine, by Merck Group (4 mentions)
Acetic acid, by Merck Group (3 mentions)
2 hydroxypropyl beta cyclodextrin mw 1460, by Merck Group (3 mentions)
Sodium acetate trihydrate, by Merck Group (3 mentions)
Ethanol p a, by Merck Group (3 mentions)
Iron 3 chloride hexahydrate, by Merck Group (3 mentions)
Sodium carbonate anhydrous, by BD (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Milli q system, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Methanol, by Carlo Erba (2 mentions)
Formic acid, by Carlo Erba (2 mentions)
Acetone, by Carlo Erba (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
N hexane, by Carlo Erba (2 mentions)
27 protocols using ethanol absolute anhydrous
1
Lipid-based Nanoparticle Synthesis and Characterization
2
Synthesis and Purification of PCL-b-PEG
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The following analytical-grade chemicals were purchased and used without further purification: 2,3,5-triiodobenzoic acid (Changzhou Ilhang Fine Chemical Co. Ltd, China), thionyl chloride (Acros Organics, 99.5%), sodium carbonate, dichloromethane (Acros Organics, 99.9%). N-methyl-2pyrrolidone (NMP, 99.5%), pyridine (99.5%) and 4dimethylaminopyridine (DMAP, 99.5%) were purchased from Acros Organics in anhydrous grade over molecular sieves in an AcroSeal packaging. Poly(vinyl alcohol) (PVAL, Sigma-Aldrich, 98% hydrolyzed, average molar mass MW = 13,000-23,000 Da) was dried at 40 °C in an oven for 24 h under vacuum before use. Tetrahydrofuran (THF, Fisher Scientific), absolute anhydrous ethanol (Carlo Erba) were used in purification processes. The polycaprolactone-block-poly(ethylene glycol) (PCL-b-PEG block copolymer) was synthesized by polymerization of caprolactone initiated by poly(ethylene glycol) monomethyl ether catalyzed by tin octanoate as reported in refs 58, 59 The degrees of polymerization of the poly(caprolactone) and. poly(ethylene glycol) blocks were 65 and 113 respectively (PCL65-b-PEG113). Deionized water of 18 Ω•cm resistivity was used for all preparations.
3
Chitosan Characterization and FITC-Dextran Probes
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Chitosan (M w 6.04 10 5 g/mol, M w /M n 1.64, DA 4.3%, from squid pen chitin) was purchased from Mahtani Chitosan Pvt. Ltd. Acetic acid, ammonium hydroxide, HEPES and FITCmodified dextran probes (4.0 10 4 g/mol, 5.00 10 5 g/mol, and 2.00 10 6 g/mol) were supplied from Sigma-Aldrich. NaOH pellets, NaCl, and absolute anhydrous ethanol were obtained from Carlo Erba Reagents.
4
Solvent Extraction and Chromatographic Analysis
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N-hexane, ethyl acetate, acetonitrile, 2-propanol, and methanol were acquired from Sigma-Aldrich (Milan, Italy), toluene was purchased from Romil ltd (Cambridge, UK), anhydrous absolute ethanol and acetic acid were purchased from Carlo Erba Reagents (Milan, Italy). Ultra-pure water was produced by a Milli-Q system (Millipore Corporation, Billerica, MA, USA).
The acronym/name and manufacturing company of compounds sought in this study, including the internal standards, are provided inTable S1 of the Supplementary Material . Stock standard solutions were prepared in methanol at a concentration of 100 µg/mL and stored at −20 °C; working solutions were weekly prepared after a proper dilution.
The acronym/name and manufacturing company of compounds sought in this study, including the internal standards, are provided in
5
Lipid Bilayer Preparation and Analysis
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Milli-Q
water (18.2 MΩ cm) was used to prepare all solutions. Cardiolipin
(CL) solution from bovine heart (∼4.7–5.3 mg mL–1 in ethanol, ≥97%) and cytochrome c from equine heart (≥95%) were obtained from Sigma-Aldrich
and stored in a freezer (−18 °C). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) powder was purchased
from Avanti Polar Lipids and stored in a freezer. The 10 mM phosphate
buffer aqueous solution at pH 7.2 was prepared by using sodium hydrogen
phosphate anhydrous ACS (99.0% min) and potassium dihydrogen phosphate
ACS (99.0% min) from Alfa Aesar. Anhydrous absolute ethanol from Carlo
Erba Reagents was used to prepare DOPC/CL lipid mixture solutions.
Concentrated hydrochloric acid (12 M, 37%, ACS Reagent) and pellets
for making sodium hydroxide solution (ACS) were obtained from VWR
and were used to adjust the pH of the phosphate buffer solution. Sodium
nitrite (>99%) was obtained from Sigma-Aldrich, and 4-nitrocatechol
(>98%) was obtained from Alfa Aesar.
water (18.2 MΩ cm) was used to prepare all solutions. Cardiolipin
(CL) solution from bovine heart (∼4.7–5.3 mg mL–1 in ethanol, ≥97%) and cytochrome c from equine heart (≥95%) were obtained from Sigma-Aldrich
and stored in a freezer (−18 °C). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) powder was purchased
from Avanti Polar Lipids and stored in a freezer. The 10 mM phosphate
buffer aqueous solution at pH 7.2 was prepared by using sodium hydrogen
phosphate anhydrous ACS (99.0% min) and potassium dihydrogen phosphate
ACS (99.0% min) from Alfa Aesar. Anhydrous absolute ethanol from Carlo
Erba Reagents was used to prepare DOPC/CL lipid mixture solutions.
Concentrated hydrochloric acid (12 M, 37%, ACS Reagent) and pellets
for making sodium hydroxide solution (ACS) were obtained from VWR
and were used to adjust the pH of the phosphate buffer solution. Sodium
nitrite (>99%) was obtained from Sigma-Aldrich, and 4-nitrocatechol
(>98%) was obtained from Alfa Aesar.
6
Silica-Based Nanoparticle Synthesis
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Ammonium hydroxide solution (ACS reagent, 28.0–30.0% NH3 basis), hexadecyltrimethylammonium bromide (CTAB, BioXtra, ≥99%), tetraethyl orthosilicate (TEOS, 99.999% trace metals basis), L-arginine (>99.5%), hydrochloric acid (ACS reagent, 37%), acetic acid (≥99.8%), and ninhydrin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anhydrous sodium acetate (>99.99%) was provided by Fluka (Buchs, Switzerland). Anhydrous absolute ethanol was purchased from Carlo Erba (Val-de-Reuil, France) and water (LC-MS grade) was provided by Merck (Billerica, MA, USA).
7
Quantitative Carbohydrate Analysis
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Methanol (HPLC grade) and ethanol absolute anhydrous were purchased from Carlo Erba (Milan, Italy); sodium carbonate, water (LC-MS, Ultra Chromasolv, Honeywell, Seelze, Germany), and acetonitrile (LC-MS Chromasolv > 99.9%) from Honeywell (Seelze, Germany). Formic acid (99% LC-MS grade) was purchased from VWR Chemicals (Radnor, PA, USA). Amberlyst 15 ion-exchange resin, Folin–Ciocalteu reagent, ABTS (2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-picrylhydrazyl) were purchased from Sigma-Aldrich (St. Louis, USA). Standard of D-(+)-saccharose molecular biology grade was purchased from AppliChem GmbH (Darmstadt, Germany); standards of D-(+)-raffinose and stachyose tetrahydrate were purchased from Supelco analytical (Darmstadt, Germany); standard of L-(−)-xylose and D-(−)-fructose were purchased from Sigma (St. Louis, MO, USA).
8
Comprehensive Carotenoid and Vitamin Analysis
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Retinyl acetate, β-carotene, α-carotene, vitamin D3 (> 95% pure), deuterated vitamin D3, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), phytic acid, DMACA, vanillin, iron III, pancreatin, ammonium thiocyanate, catechins, α-amylase, pepsin, bile, ferric chloride and sodium carbonate were purchased from Sigma Aldrich (Saint-Quentin-Fallavier, France). Lutein, lycopene, phytoene and phytofluene were purchased from Extrasynthèse (Genay, France). Soyasaponin I was generously supplied by Stéphane Georgé, CTCPA Avignon. Canned tomato pulp, red bell peppers and Isio4 oil (Lesieur, Asnières, France) were purchased from a local supermarket (Marseille, France). Methanol, acetonitrile, hexane, dichloromethane, methyl-tert-butylether, acetone, ethanol absolute anhydrous, sulfuric acid, hydrochloric acid, nitric acid, formic acid, hydrogen peroxide were purchased from CarloErba Reagents (Peypin, France).
9
Formulation and Characterization of Fenretinide
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N-4-hydroxyphenyl-retinamide (fenretinide, 4-HPR) was purchased from Olon Spa (Milan Italy); lenalidomide from AstaTech Inc (PA, USA); soy L-α-phosphatidylcholine, glyceryl tributyrate, 2-hydroxypropyl beta cyclodextrin (Mw 1460) and KOH from Sigma-Aldrich; ethanol absolute anhydrous from Carlo Erba Reagents (Milan Italy).
10
Analytical Reagents for Phytochemical Analysis
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Ethanol p.a, hydrochloric acid (370 g·L−1), iron (II) sulphate heptahydrate, iron (III) chloride hexahydrate, sodium acetate trihydrate, were purchased from Merck (Darmstadt, Germany). Folin–Ciocalteu reagent, and potassium hexacyanoferrate (III), gallic acid (990 g·L−1) were purchased from Sigma (Sternheim, Germany). Sodium carbonate anhydrous was obtained from BDH (Poole, UK), while 2,4,6-tris (2-pyridyl)-S-triazine (TPTZ, 990 g·L−1) and ferric chloride were acquired from Fluka (Buchs, Germany), and anhydrous sodium sulphate from Panreac (Barcelona, Spain). Ethanol absolute anhydrous was purchased from Carlo Erba (Marseille, France). Luteolinidin chloride was acquired from Chromadex (Los Angeles, CA, USA). Maltodextrin (DE 16.5–19.5) and Arabic gum was purchased from Sigma-Aldrich (Darmstadt, Germany). All other unlabelled chemicals and reagents were analytical, or HPLC-MS Optima grade from Fisher Scientific (Waltham, CA, USA).
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