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F0299

Manufactured by Bio-Serv
Sourced in United States

The F0299 is a laboratory equipment designed to measure the viscosity of fluids. It utilizes a rotating spindle to determine the resistance of a fluid to flow, providing an objective assessment of its viscosity.

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7 protocols using f0299

1

Operant Conditioning Chambers for Sucrose and Polycose Trials

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Training and testing took place in a set of 16 identical MED Associates operant chambers enclosed in sound- and light-resistant shells. Each chamber was equipped with two pumps fitted with a syringe that delivered 0.1 ml of a 20% sucrose solution or a 20% polycose solution into a recessed magazine in the chamber. The chambers were also equipped with two food pellet dispensers that delivered either grain (45 mg; # F0165, BioServ Technologies), purified (45 mg; # F0021, BioServ Technologies) or chocolate purified pellets (F0299; BioServ, Flemington, NJ, USA) when activated. Two retractable levers were located to the left and the right of the magazine. A 3 W, 28 V house light provided illumination of the operant chamber, and an infrared photo beam spanning across the magazine opening detected head entries. Chambers contained a 28 V DC mechanical relay that was used to deliver a 2 Hz clicker stimulus and a white noise generator (80 dB). Two computers running MED Associates proprietary software (Med-PC; Fairfax, VT, USA) controlled the equipment and recorded responses. To achieve outcome devaluation via sensory-specific satiety, each rat was placed in an individual box located in a separate feeding room from where training and testing took place.
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2

Rat Spatial Learning and Memory

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Sixteen male Long–Evans rats (Rattus norvegicus; Harlan, Indianapolis, IN; 73 days old and 295 g on average, at the start of the experiment) were individually housed with light onset and offset in the colony at 7 a.m. and 7 p.m. EST, respectively. Two rats were excluded from testing with delays (one rat learned to escape the maze and another failed to visit pellet locations during initial stages of training); therefore, we report data from fourteen rats. The rats received 45-mg chow and chocolate pellets (F0165 and F0299, respectively; Bio-Serv, Frenchtown, NJ) during experimental sessions and 15 g/day of 5012-Rat-Diet (PMI Nutrition International, St. Louis, MO) after completing each session. Water was available ad lib, except when the rat was in the maze. All procedures were approved by the Institutional Animal Care and Use Committee at Indiana University Bloomington (protocol 13-012) and followed national guidelines.
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3

Nicotine Self-Administration in Rats: Pharmacological Modulation

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The rats were trained to respond for food pellets (45 mg, F0299, Bio-Serv, Frenchtown, NJ) in operant chambers (Med Associates, St. Albans, VT) under a fixed-ratio 1, time-out 10s (FR1-TO10s) schedule. After completing the food training sessions, the rats were implanted with a catheter in the jugular vein. The rats were allowed to self-administer nicotine for nine days (daily 1-h sessions). The rats self-administered 0.03 mg/kg/inf of nicotine under an FR1-TO10s schedule for three days and then 0.06 mg/kg/inf of nicotine under an FR1-TO60s for six days. The effect of flupentixol (0, 0.5, 0.75, and 1 mg/kg, IP) and L-741626 (0, 0.3, 1, and 3 mg/kg, SC) on nicotine intake was investigated in daily 1 h sessions (0.06 mg/kg/inf). The rats were fed 90–95 percent of their baseline food intake immediately after the nicotine self-administration sessions.
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4

Spatial Navigation in Male Rats

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Six male Long-Evans rats (Rattus norvengicus; Envigo, Indianapolis, IN; 49 days old and 225 g on average, at the start of the experiment) were individually housed with light onset and offset in the colony at 7:30 and 19:30 EST, respectively. Rewards consisted of 45-mg chow and chocolate pellets (F0165 and F0299, respectively; BioServ, French town, NJ). Rats received daily rations of 5012-Rat-Diet (PMI Nutrition International, St. Louis, MO) after the completion of each session conducted five days per week. Water was available ad lib, except when the rat was in the maze. All procedures were approved by the Institutional Animal Care and Use Committee at Indiana University Bloomington (protocol 15-019) and followed national guidelines.
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5

Operant Conditioning in Rat Food Studies

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Rats were trained to press a lever for food pellets in the operant chambers (Med Associates, St. Albans, VT). Food training was conducted before the catheters were implanted and also before the rats were treated with SCH 23390 or A77636 in the food studies. Responding on the active lever (right lever, RL) resulted in the delivery of a food pellet (45 mg, F0299, Bio-Serv, Frenchtown, NJ). Responding on the inactive lever (left lever, LL) was recorded but did not have scheduled consequences. Food delivery was paired with a cue light, which remained illuminated throughout the time-out (TO) period. The food training sessions were conducted for 10 days. Instrumental training started under an FR1-TO1s reinforcement schedule for 5 days (30 min session per day). After the fifth food training session, the rats were singly housed and remained singly housed for the rest of the study. On day 6, the time-out period was increased to 10 s. The rats were allowed to respond for food pellets under the FR1-TO10s schedule (20 min sessions) for 5 days. Both levers were retracted during the 10 s time-out period. During the food training period, the rats were fed 90–95% of their baseline food intake in the home cage.
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6

Spatial Memory in Long-Evans Rats

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Seven male Long-Evans rats (Rattus norvengicus; Harlan, Indianapolis, IN; ~1.5 years; 451 g., on average, at the start of the experiment) were individually housed with light onset and offset in the colony at 7 a.m. and 7 p.m. EST, respectively. One rat failed to revisit the replenishment location at initial stages of training and was excluded from Experiments 2 and 3. The rats received 45-mg chow and chocolate pellets (F0165 and F0299, respectively; Bio-Serv, French town, NJ) during experimental sessions and 15 g/day of 5012-Rat-Diet (PMI Nutrition International, St. Louis, MO) after completing each session. Water was available ad lib, except when the rat was in the maze. Prior to the onset of the present study, the rats received training in the source memory task described in Crystal and Smith [21 (link)].
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7

Operant Responding and Locomotor Effects

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Adult male and female rats were trained to respond for food pellets (45 mg, F0299, Bio-Serv, Frenchtown, NJ) in operant chambers (Med Associates, St. Albans, VT) under an FR1-TO10s schedule. After the food training sessions, the effects of flupentixol (0, 0.5, 0.75, and 1 mg/kg, IP) and L-741626 (0, 0.3, 1, and 3 mg/kg, SC) on operant responding for food pellets was investigated in daily 20-min sessions under an FR1-TO10s schedule (Expt. 2). The rats were fed 90–95 percent of their baseline food intake in the home cage after operant responding for food. After the food study, the rats were fed ad-lib, and the effects of flupentixol and L-741626 on locomotor activity were investigated (Expt. 3). The rats were habituated to the small open field on three consecutive days (20-min sessions) and then the effects of flupentixol (0, 0.5, 0.75, and 1 mg/kg, IP) and L-741626 (0, 0.3, 1, and 3 mg/kg, SC) on locomotor activity were investigated in 20-min sessions.
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