Elecsys estradiol 2

We collected fasting venous blood samples for measurement of adrenal androgens such as testosterone (normal range, 3–10 ng/dL), dehydroepiandrosterone (DHEA) (normal range, 20–275 ng/dL), androstenedione (normal range, 8–50 ng/dL), and estradiol (normal range lower than 1.5 ng/dL). Testosterone (KIP1709; DIAsource ImmunoAssays S.A., Nivelles, Belgium), DHEA (IMaa38; IMMUNOTECH, Marseille, France), and androstenedione (KIP0451; DIAsource ImmunoAssays, S.A., Louvain-la-Neuve, Belgium) were measured using commercial kits.
The estradiol level in blood was measured only in female subjects (n = 94) by using a competitive electrochemiluminescence immunoassay method with an Elecsys E170 analyzer (Elecsys Estradiol II; Roche Diagnostics GmbH, Mannheim, Germany).

All clinical variables of the study participants were assessed when they first visited the outpatient department. Blood samples for biochemical laboratory analyses were taken from all subjects in the early follicular phase after overnight fasting. Serum E1 was measured using the Dsl-8700 Estrone ELISA kit (Beckman Coulter, Brea, CA, USA) and serum E2 was measured using Elecsys Estradiol II (Roche, Indianapolis, IN, USA) [23 (link)]. Serum insulin and glucose levels were analyzed using an Elecsys Insulin assay (Roche) and an L-Type GluI device (Wako, Osaka, Japan), respectively. Cholesterol and triglyceride levels were measured using Pureauto S (Sekisui, Tokyo, Japan), and serum high-density lipoprotein and low-density lipoprotein levels were measured using Cholestest (Sekisui) [30 (link)]. Both intra- and inter-assay coefficients of variation for all assays were below 8%.