Animals were dark-adapted for more than 1 h before experiments. Under a dim red light, a goldfish was double-pithed and eyes were enucleated. The following procedure was performed under a stereomicroscope equipped with infrared (IR) image converter (C5100, Hamamatsu photonics) and IR illuminator (HVL-IRM, Sony). After the cornea and lens were ablated, the eye cup was treated with a mixture of hyaluronidase and collagenase (4 mg/mL each, Sigma-Aldrich Corp.) for a few min. A small cut was made at the dorsal part of the eye cup as a landmark and thus the ventral retina isolated from the pigment epithelium was properly oriented and positioned on the multi-electrode array.
Hvl irm
Manufactured by Sony
The HVL-IRM is a multi-interface remote commander for Sony's professional camera systems. It provides remote control functionality for various camera operations, such as start/stop recording, aperture adjustment, and shutter speed control. The device connects to the camera via a wired interface and allows users to make adjustments from a distance, enhancing the flexibility of camera setups.
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Lab products found in correlation
3 protocols using hvl irm
1
Goldfish Retina Extraction Protocol
2
Comparing Nectar-Drinking Behaviors of Glossophagine and Lonchophylline Bats
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Using high-speed video recordings, we compared the nectar-drinking behavior of the Pallas’ long-tongued bat G. soricina (the most common glossophagine species with a brush-tip tongue) to that of the orange nectar-feeding bat L. robusta (a lonchophylline species with a grooved tongue). Experiments with G. soricina were performed in an experimental chamber (4.8 m by 2.4 m by 2.2 m) between September 2009 and August 2010 using bats from a captive colony at the University of Ulm (Ulm, Germany). L. robusta bats captured temporarily from the wild were tested in a flight tent (4 m by 4 m by 2.5 m) at the Bocas del Toro Field Station of the Smithsonian Tropical Research Institute (Balboa, Panama) in March 2009.
Bats (L. robusta, n = 3; G. soricina, n = 4) were recorded visiting a glass tube (10.3 mm by 5.5 mm by 100 mm) filled with artificial nectar (honey water, 17% w/w sugar concentration) up to 20 mm below the opening. All video recordings were made under infrared light-emitting diode light (Sony HVL-IRM) using a black-and-white high-speed camera (Optronis Camrecord 600x2) with Nikkor 60- or 100-mm macro lenses (Nikon) set to 500 to 750 frames/s for 1/1000 to 1/3003 s of exposure. Tongue-tip insertion into the feeder was tracked on all available video frames using ImageJ software (25 ).
Bats (L. robusta, n = 3; G. soricina, n = 4) were recorded visiting a glass tube (10.3 mm by 5.5 mm by 100 mm) filled with artificial nectar (honey water, 17% w/w sugar concentration) up to 20 mm below the opening. All video recordings were made under infrared light-emitting diode light (Sony HVL-IRM) using a black-and-white high-speed camera (Optronis Camrecord 600x2) with Nikkor 60- or 100-mm macro lenses (Nikon) set to 500 to 750 frames/s for 1/1000 to 1/3003 s of exposure. Tongue-tip insertion into the feeder was tracked on all available video frames using ImageJ software (25 ).
3
Goldfish Retina Isolation for Electrophysiology
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Goldfish were dark-adapted for more than 1 h before experiments. Under a dim red light, a goldfish was double-pithed, and eyes were enucleated. The following procedure was performed under a stereomicroscope equipped with infrared (IR) image converter (C5100, Hamamatsu photonics) and IR illuminator (HVL-IRM, Sony). After the cornea and lens were ablated, the eye cup was treated with a mixture of hyaluronidase and collagenase (4 mg/mL each, Sigma-Aldrich Corp.) for a few min. A small cut was made at the dorsal part of the eye cup as a landmark and thus the ventral retina isolated from the pigment epithelium was properly oriented and positioned on the multi-electrode array or in the recording chamber for whole-cell recordings.
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