Imazapyr

Manufactured by Merck Group

Imazapyr is a broad-spectrum herbicide used in lab settings. It is an active ingredient that inhibits the enzyme acetolactate synthase, which is essential for plant growth. Imazapyr is commonly used in research and development applications.

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Lab products found in correlation

Chlorimuron ethyl, by Merck Group (1 mentions)

2 protocols using imazapyr

Somatic Embryo Maturation and Germination

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All media recipes are described by Lowe et al. (2016 (link)). For the rapid methods described above 0.1 mg L⁻¹ imazapyr (Sigma-Aldrich®; St. Louis, MO). was added to the somatic embryo maturation and germination steps.

Lowe K., La Rota M., Hoerster G., Hastings C., Wang N., Chamberlin M., Wu E., Jones T, & Gordon-Kamm W. (2018). Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis. In Vitro Cellular & Developmental Biology, 54(3), 240-252.

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Antifungal Compound Sensitivity Assay

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HPLC-grade chemicals imazapyr, imazamox, and chlorimuron ethyl were purchased from Sigma-Aldrich, and dissolved in DMSO. Gamborg’s minimal media plates with increasing concentrations from 0.5 µg/ml to 256 µg/ml of each chemical were prepared. Three replicate cultures were prepared for each of D22, the ilv2 mutant, and the ilv2 complemented strain at each concentration. Equal-sized mycelial plugs were placed in the center of each plate. Following 10 days of growth at 22 °C, colony diameter measurements were taken on three horizontal axes on each plate.

Chong N.F., Van de Wouw A.P, & Idnurm A. (2024). The ilv2 gene, encoding acetolactate synthase for branched chain amino acid biosynthesis, is required for plant pathogenicity by Leptosphaeria maculans. Molecular Biology Reports, 51(1), 682.

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