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Ni agarose his tagged protein purification kit

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The Ni-agarose His-Tagged Protein Purification Kit is a laboratory equipment used for the purification of recombinant proteins with a histidine (His) tag. The kit contains Ni-agarose resin, which specifically binds to the His-tag on the target protein, allowing for its separation from other cellular components. This product facilitates the efficient isolation and concentration of the desired His-tagged protein for further analysis or applications.

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Lab products found in correlation

Lightshift chemiluminescent emsa kit, by Thermo Fisher Scientific (3 mentions) Annealing buffer, by Beyotime (1 mentions) Annealing buffer for dna oligos, by Beyotime (1 mentions) Pierce gst protein interaction pull down kit, by Thermo Fisher Scientific (1 mentions) Bl21 de3, by Takara Bio (1 mentions) Pgex 4t 3, by Cytiva (1 mentions) Pierce gst spin purification kit, by Thermo Fisher Scientific (1 mentions)

4 protocols using ni agarose his tagged protein purification kit

1

Electrophoretic Mobility Shift Assay Protocol

Cited 1 time
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The EMSA was performed using the LightShift Chemiluminescent EMSA Kit (Thermo Scientific, Waltham, MA, USA) as described by Jiang et al. [61 (link)]. The recombinant protein was purified using the Ni-agarose His-Tagged Protein Purification Kit (CWbiotech, Beijing, China).
Zhang Z., Tian C., Zhang Y., Li C., Li X., Yu Q., Wang S., Wang X., Chen X, & Feng S. (2020). Transcriptomic and metabolomic analysis provides insights into anthocyanin and procyanidin accumulation in pear. BMC Plant Biology, 20, 129.
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2

MdMYB1 Protein Purification and EMSA

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The EMSAs were performed using the LightShift Chemiluminescent EMSA Kit (Thermo Scientific). The CDS of MdMYB1 was cloned into the expression vector pET-32a (EMD Biosciences, Novagen, San Diego, CA, USA). The MdMYB1 recombinant protein was expressed in Escherichia coli strain BL21 (Tiangen) and purified using a Ni-agarose His-Tagged Protein Purification Kit (CWbiotech). All probes were synthesized and labeled by Sangon Biotech Co. Ltd. (Shanghai, China). Double-stranded probes were synthesized using annealing buffer (Beyotime).
Jiang S., Chen M., He N., Chen X., Wang N., Sun Q., Zhang T., Xu H., Fang H., Wang Y., Zhang Z., Wu S, & Chen X. (2019). MdGSTF6, activated by MdMYB1, plays an essential role in anthocyanin accumulation in apple. Horticulture Research, 6, 40.
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3

Electrophoretic Mobility Shift Assay for MdAGO4-1/2

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Electrophoretic mobility shift assays (EMSAs) were conducted using the LightShift Chemiluminescent EMSA Kit (Thermo Scientific). MdAGO4‐1/2 were cloned into the expression vector pET32a. The AGO4‐1/2 recombinant protein was expressed in E. coli strain BL21 (DE3) and purified using a Ni‐agarose His‐Tagged Protein Purification Kit (CW Biotech). All promoter probes were synthesized and labelled by the Sangon Biotech Co. Double‐stranded probes were synthesized using annealing buffer for DNA oligos (Beyotime, Shanghai, China). The partial primers used for EMSAs are listed in Table S1.
Jiang S., Wang N., Chen M., Zhang R., Sun Q., Xu H., Zhang Z., Wang Y., Sui X., Wang S., Fang H., Zuo W., Su M., Zhang J., Fei Z, & Chen X. (2020). Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple. Plant Biotechnology Journal, 18(8), 1736-1748.
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4

Purification and Interaction of GST-ERα and His-TCF21

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GST-ERα (Δ2) was prepared by cutting Flag-ERα (Δ2) with BamHI and XhoI, and the fragment was then inserted into pGEX-4T3 (Amersham Pharmacia). The GST and GST-fusion protein were expressed in BL21(DE3) (Takara), and purified by Pierce GST Spin Purification Kit (Thermo scientific). His-TCF21 was prepared by cutting Flag-TCF21 with EcoRI and XhoI, and then the fragment was inserted into pET32a(+) (Novagen). His-TCF21 protein was expressed in BL21 and purified by Ni-Agarose His-tagged Protein Purification Kit (CW Biotech). GST pull-down assay was performed using a Pierce GST Protein Interaction Pull-Down Kit (Thermo scientific).
Ao X., Li S., Xu Z., Yang Y., Chen M., Jiang X, & Wu H. (2016). Sumoylation of TCF21 downregulates the transcriptional activity of estrogen receptor-alpha. Oncotarget, 7(18), 26220-26234.
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