All animal experiments were approved and performed according to the guidelines and regulations of the local committees for animal care at Uppsala University. Male C57BL/6 or C57BL/6 (nu/nu) mice (Taconic, Ry, Denmark) were used in all experiments. The animals were housed in the Animal Department at the Biomedical Centre (Uppsala, Sweden) and had free access to pelleted food and tap water. Mouse pancreatic β-TC6 cells (ATCC; Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL benzylpenicillin, 0.1 mg/mL streptomycin (Roche Diagnostics Scandinavia, Bromma, Sweden) in humidified air and 5% CO2 at 37°C. Medium was changed every third day, and the cells were used in passage 3–8.
C57bl 6 nu nu mice
Manufactured by Taconic Biosciences
Sourced in Denmark
C57BL/6 (nu/nu) mice are an inbred strain of laboratory mice that are athymic, meaning they lack a functional thymus gland. This results in a lack of mature T cells, which leads to a compromised immune system. These mice are commonly used in research applications that require an immunodeficient animal model.
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2 protocols using c57bl 6 nu nu mice
1
Experimental Mouse Model for Diabetes Research
2
Standardized Mouse Housing Conditions
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All experiments were approved by the animal ethical board of Uppsala County. Adult male C57BL/6 and C57BL/6 nu/nu mice weighing ~25 g were purchased from Taconic (Ry, Denmark). C57BL/6 mice expressing enhanced green fluorescent protein (EGFP) under the Flk-1 (VEGFR2) promoter (Flk1-GFP mice) or under the mouse insulin 1 promoter (MIP-GFP mice) were obtained from local breeding colonies. All animals were housed under standardized conditions with ad libitum access to food and water.
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