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Microlyte

Manufactured by Harvard Apparatus
Sourced in United States

The Microlyte is a precision liquid handling instrument designed for accurately dispensing small volumes of liquid samples. It features adjustable volume settings and can be used for a variety of applications in laboratory settings.

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3 protocols using microlyte

1

Measuring Startle Blink Reflex with EMG

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FPS was measured through electromyography (EMG) of the right orbicularis oculi muscle. Two 4-mm Ag/AgCl electrodes filled with conductive electrolyte gel (Microlyte, Coulbourn Instruments, Holliston, Massachusetts) were placed 1 cm below the pupil and 1 cm below the lateral canthus, and a third (ground) electrode was placed on the forehead26 (link). Acoustic startle probes (40 ms white noise, 100 dBA) were presented binaurally through headphones (Sennheiser HD 202). The EMG signal was sampled at 1000 Hz and amplified using an isolated bioamplifier with band-pass filter (Lablinc v75-04, Coulbourn Instruments) with a high pass filter of 13 Hz and a low pass filter of 500 Hz. The signal was rectified and smoothed online at a time constant of 20 ms, using a 4-channel integrator (Lablinc v76-24, Coulbourn Instruments). The analogue output was then digitized by a 16-bit AD converter (National Instruments, NI-6221, Austin, Texas). Offline processing was completed with PSPHA27 (link). Blink amplitude was determined by subtracting a 20-ms baseline (0–20 ms following probe onset) from the peak response in a 21–200 ms window following probe onset. To standardize the data, means and standard deviations from the first testing session were used to calculate within-participant Z-scores.
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2

Transcranial Direct Current Stimulation Protocol

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During each testing block (Figure 1), tDCS was applied to the scalp over the target using a battery-powered Soterix 1X1 tDCS device (Figure 2; Soterix Medical Inc., New York, NY, USA). This single-channel device has one stimulating and one reference electrode. The device delivers a maximum of 2 mA of direct current stimulation for no more than 20 minutes. Stimulation was applied with carbon-rubber electrodes enclosed in disposable 3 × 5 cm sponge pockets. The sponges were saturated in normal saline (0.9% NaCl) and affixed to the head with a rubber headband adjusted to fit snugly but without discomfort. To enhance the connection, standard conductive gel (Microlyte, Coulbourn Instruments, Whitehall, PA, USA) was applied between the scalp and the sponge pocket. We started tDCS seven minutes before the first experimental task for participant acclimation; this was two to three minutes longer than similar studies demonstrating tDCS-associated effects on cognitive task performance (26 ,27 ).
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3

Transcranial Direct Current Stimulation Protocol

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To target left DLPFC, the stimulating electrode was placed on the scalp at F3 per the 10-20 electroencephalography (EEG) system. The return electrode was placed over the contralateral mastoid process. Electrodes were carbon rubber and enclosed in 3 cm by 5 cm sponge pockets saturated in normal saline (0.9% NaCl) and held on the scalp with rubber headbands adjusted to be snug but comfortable. No hair was shaved. To enhance connection, conductive gel (Microlyte, Coulbourn Instruments, Whitehall, PA, USA) was applied to scalp beneath the sponge pocket. Electrodes were then connected to a battery-powered Soterix 1×1 bipolar tDCS device (Soterix Medical Inc., New York, NY, USA). Before stimulation, impedance of the electrode-to-scalp interface was checked with the device to ensure that it remained at or below a predetermined safety cutoff level. Amplitude of tDCS was ramped up to 2 mA over 30 seconds and then maintained for 20 minutes before being ramped back down to 0 mA. Current was otherwise lowered by tDCS staff only in the event of poor tolerability. We began tDCS seven minutes before the first experimental task to acclimate participants fully to stimulation; similar studies of tDCS-associated effects on cognitive task performance (38 ,39 ) used acclimation periods of four to five minutes.
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