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Anti human caspase 1

Manufactured by Santa Cruz Biotechnology
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Anti-human Caspase-1 is a laboratory reagent used for the detection and quantification of human caspase-1 protein. Caspase-1 is an enzyme involved in the inflammatory response and the initiation of programmed cell death (apoptosis). This product can be used in various research applications that require the measurement of caspase-1 levels or activity.

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Lab products found in correlation

Anti asc, by Santa Cruz Biotechnology (2 mentions) Anti phospho s242 pyrin, by Abcam (2 mentions) Anti human gsgmd, by Merck Group (2 mentions) Anti β actin clone, by Merck Group (2 mentions) Mini protease inhibitor mixture, by Roche (2 mentions) Anti pyrin, by Adipogen (2 mentions) Transblot turbo midi size pvdf membranes, by Bio-Rad (2 mentions) Acrylamide gel, by Bio-Rad (2 mentions) Anti human il 1β, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (2 mentions) Anti flag m2 affinity gel, by Merck Group (2 mentions) Sodium fluoride, by Merck Group (2 mentions) Nigericin, by Merck Group (1 mentions) Sheep anti mouse igg hrp, by Cytiva (1 mentions) Anti mouse il 1β, by R&D Systems (1 mentions) 5 and 6 carboxyfluorescein diacetate succinimidyl ester cfse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594conjugated goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg horseradish peroxidase hrp, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)

3 protocols using anti human caspase 1

1

Inflammasome Activation Assay Protocol

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PMA (cat# P1585), ATP (cat# A7699), nigericin (cat# N7143), 4′,6-diamidino-2-phenylindole (DAPI; cat# D9542), and cytochalasin D (cat# C8273) were purchased from Sigma-Aldrich (St Louis, MO, USA), anti-ASC (cat# SC-22514-R), anti-human caspase-1 (cat# SC-56036), anti-mouse caspase-1 (cat# SC-514), anti-human IL-1β (cat# SC-32294), anti-α-tubulin (cat# SC-32293), anti-GAPDH (cat# SC-32233), and goat anti-rabbit IgG-horseradish peroxidase (HRP; cat# SC-2004) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-NLRP3 (cat# AG-20B-0014) from Adipogen (San Diego, CA, USA), anti-mouse IL-1β (cat# AF-401-NA) from R&D Systems Inc. (Minutesneapolis, MN, USA); sheep anti-mouse IgG-HRP (cat# NA931) from Amersham (Amersham, UK), (5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE; cat# C1157) and Alexa Fluor-594 conjugated goat-anti-mouse IgG (H+L) (cat# A-11005) from Invitrogen (Carlsbad, CA, USA); and fluoresbrite yellow green carboxylate microspheres (1-μm YG beads, cat# 15702) from Polysciences Inc. (Warrington, PA, USA).
Chung I.C., OuYang C.N., Yuan S.N., Lin H.C., Huang K.Y., Wu P.S., Liu C.Y., Tsai K.J., Loi L.K., Chen Y.J., Chung A.K., Ojcius D.M., Chang Y.S, & Chen L.C. (2019). Pretreatment with a Heat-Killed Probiotic Modulates the NLRP3 Inflammasome and Attenuates Colitis-Associated Colorectal Cancer in Mice. Nutrients, 11(3), 516.
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2

Pyrin-ASC Inflammasome Activation

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Cells were lysed in 25mM Tris HCl, 150mM NaCl, 1mM EDTA and 0.1% NP-40 buffer containing Mini Protease Inhibitor Mixture (Roche) and sodium fluoride (Sigma) by a quick freezing and thawing step. Flag-Pyrin was immuno-precipitated using anti Flag M2 affinity gel (Sigma). ASC was cross-linked in the insoluble pellet using DSS (Disuccinimidyl suberate, ThermoFisher #21655) 2 mM (1 h at 37°C). Proteins were separated by SDS/PAGE on precast 4–15% acrylamide gels (Bio-rad) and transferred to TransBlot® Turbo™ Midi-size PVDF membranes (Bio-rad). Antibodies used were mouse monoclonal anti-FLAG® (Sigma-Aldrich, clone M2; 1:1,000 dilution), anti-Pyrin (Adipogen, AL196, 1: 1,000 dilution), anti-phospho S242 Pyrin (Abcam, ab200420; 1:1,000 dilution) (Gao et al., 2016 (link)), anti-human Caspase-1 (Santa Cruz, sc515, 1: 1,000 dilution), anti-human GSGMD (sigma, HPA044487, 1: 1,000 dilution), anti-human IL-1β (Cell signaling, #12703, 1: 1,000 dilution), anti-ASC (Santa Cruz, sc22514R, 1:1,000 dilution). Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
Magnotti F., Chirita D., Dalmon S., Martin A., Bronnec P., Sousa J., Helynck O., Lee W., Kastner D.L., Chae J.J., McDermott M.F., Belot A., Popoff M., Sève P., Georgin-Lavialle S., Munier-Lehmann H., Tran T.A., De Langhe E., Wouters C., Jamilloux Y, & Henry T. (2022). Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell reports, 41(2), 111472.
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3

Pyrin-ASC Inflammasome Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 25mM Tris HCl, 150mM NaCl, 1mM EDTA and 0.1% NP-40 buffer containing Mini Protease Inhibitor Mixture (Roche) and sodium fluoride (Sigma) by a quick freezing and thawing step. Flag-Pyrin was immuno-precipitated using anti Flag M2 affinity gel (Sigma). ASC was cross-linked in the insoluble pellet using DSS (Disuccinimidyl suberate, ThermoFisher #21655) 2 mM (1 h at 37°C). Proteins were separated by SDS/PAGE on precast 4–15% acrylamide gels (Bio-rad) and transferred to TransBlot® Turbo™ Midi-size PVDF membranes (Bio-rad). Antibodies used were mouse monoclonal anti-FLAG® (Sigma-Aldrich, clone M2; 1:1,000 dilution), anti-Pyrin (Adipogen, AL196, 1: 1,000 dilution), anti-phospho S242 Pyrin (Abcam, ab200420; 1:1,000 dilution) (Gao et al., 2016 (link)), anti-human Caspase-1 (Santa Cruz, sc515, 1: 1,000 dilution), anti-human GSGMD (sigma, HPA044487, 1: 1,000 dilution), anti-human IL-1β (Cell signaling, #12703, 1: 1,000 dilution), anti-ASC (Santa Cruz, sc22514R, 1:1,000 dilution). Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
Magnotti F., Chirita D., Dalmon S., Martin A., Bronnec P., Sousa J., Helynck O., Lee W., Kastner D.L., Chae J.J., McDermott M.F., Belot A., Popoff M., Sève P., Georgin-Lavialle S., Munier-Lehmann H., Tran T.A., De Langhe E., Wouters C., Jamilloux Y, & Henry T. (2022). Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell reports, 41(2), 111472.
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