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Athymic nu nu mice

Manufactured by Charles River Laboratories
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Athymic nu/nu mice are laboratory rodents that lack a thymus gland, resulting in a compromised immune system. These mice are widely used in biomedical research to study various disease models and test experimental therapies.

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Lab products found in correlation

Pbs matrigel, by BD (3 mentions) Matrigel, by BD (3 mentions) Transit 2020 transfection reagent, by Mirus Bio (2 mentions) Facsaria 3, by BD (2 mentions) Alzet osmotic minipumps model 1004, by Durect (1 mentions) Polyethylene glycol 400, by Merck Group (1 mentions) Matrigel basement membrane matrix, by BD (1 mentions) Living image 4, by PerkinElmer (1 mentions) C57bl 6, by Charles River Laboratories (1 mentions) Doxycycline hyclate, by Thermo Fisher Scientific (1 mentions) Alzet osmotic minipump, by Durect (1 mentions) B6129sf1 j mice, by Jackson ImmunoResearch (1 mentions) Fox chase scid mice, by Charles River Laboratories (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Chloroquine, by Merck Group (1 mentions) Hek293t, by Thermo Fisher Scientific (1 mentions) Mda mb 453, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions)

35 protocols using athymic nu nu mice

1

Studying Tumor Angiogenesis in Mice

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Seven-weeks-old (20–22 g body weight) female athymic nu/nu mice were obtained from Charles River animal facility. The mice were housed in sterilized filter-topped cages and maintained in a pathogen-free animal facility. SF767 cells were implanted by subcutaneous injection of 5.106 cells in 200 μL of PBS into the right flank of the mice. Once tumors were established (V≈80 mm3), mice were treated twice a week by intratumoral injection of 5 μg control siRNA, EFNB3 siRNA and/or EPHA4 siRNA, diluted in JetPEI in vivo transfectant. Tumor sizes were measured with a caliper. The tumor volume was calculated with the formula v = 0.5*(length*width2). At the end of the treatment, tumors were harvested and fixed in formol. After CD31 staining, microvessels density was quantified in blind for each tumor using Histolab software (n = 100 to 300 fields per tumor slide). Vascularization of treated or untreated size-matched tumors was also quantified after microfil perfusion of mice using a microcomputed tomography system, and expressed as the percentage of peripheric blood vessels volume to tumor one's.
Royet A., Broutier L., Coissieux M.M., Malleval C., Gadot N., Maillet D., Gratadou-Hupon L., Bernet A., Nony P., Treilleux I., Honnorat J., Liebl D., Pelletier L., Berger F., Meyronet D., Castets M, & Mehlen P. (2017). Ephrin-B3 supports glioblastoma growth by inhibiting apoptosis induced by the dependence receptor EphA4. Oncotarget, 8(14), 23750-23759.
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2

Subcutaneous Implantation of TGF-β3 Constructs

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Constructs with tethered TGF-β3 were thrice rinsed in PBS prior to implantation in athymic (nu/nu) mice (Charles River). Mice were anesthetized with isofluorane. Two incisions were made to form dorsal subcutaneous pockets above each limb. A total of four pockets were prepared each with a single implant (4 implants/mouse). Incisions were closed with staples that were removed after 10 days. After 15, 43, and 64 days mice were euthanized via CO2 asphyxiation and cervical dislocation. All protocols followed were approved by the CU Boulder Institutional Animal Care and Use Committee and follow all guidelines from the NIH.
Schneider M.C., Chu S., Randolph M.A, & Bryant S.J. (2019). An In Vitro and In Vivo Comparison of Cartilage Growth in Chondrocyte-Laden Matrix Metalloproteinase-Sensitive Poly(Ethylene Glycol) Hydrogels with Localized Transforming Growth Factor β3. Acta biomaterialia, 93, 97-110.
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3

Tadalafil Inhibits Tumor Growth in Mice

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Eight-week old female athymic nu/nu mice were obtained from Charles River Laboratories (Wilmington, MA). Mice were housed 4/cage under sterile conditions, and acclimated for 7–10 days before experiments. The animal use protocol (#04-06-29-01) was approved by the University of Cincinnati Institutional Animal Care & Use Committee. CAL27 cells were suspended 1:1 in PBS/Matrigel (BD Biosciences, Franklin Lakes, NJ) and 2 × 106 cells/100 μL were inoculated sc into the flank. Tumor dimensions were measured twice/week, and volume was calculated as length × width2 × 0.52. When tumors reached ~200 mm3, Alzet osmotic mini-pumps (model 1004, Durect Corporation; Cupertino, CA) with a 100 μL reservoir and rated for a continuous delivery at 0.09 μL/hr for 4 weeks were implanted sc. The pumps delivered vehicle (polyethylene glycol 400; Sigma) or Tadalafil (1 mg/kg/day). Each treatment included 12 mice. Tumor volumes were measured for an additional 3 weeks, at which time mice were euthanized and tumors were removed and weighed.
Tuttle T.R., Mierzwa M.L., Wells S.I., Fox S.R, & Ben-Jonathan N. (2015). The cyclic GMP/protein kinase G pathway as a therapeutic target in head and neck squamous cell carcinoma. Cancer letters, 370(2), 279-285.
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4

Xenograft Tumor Model with ETS1-DN

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Athymic Nu/Nu mice (6–7 month old females, Charles River Laboratories,Roanoke, IL) and treated and maintained in accordance to institutional guidelines. A375 cells were transfected with either the pcDNA3-GFP or pcDNA3-ETS1-DN-IRES-GFP vectors with TransIT-2020 Transfection Reagent (Mirus, Madison, WI) according to manufacturer’s instruction. Eighteen hours post-transfection, cells were trypsinized and resuspended at 20×106 cells/mL in DMEM media containing 20% FBS. GFP-positive cells were collected by the University of Chicago Flow Cytometry Core Facility using the FACSAria III (BD, Franklin Lakes, NJ). Cell counts and viability were confirmed using hemacytometer readings and trypan blue exclusion. Sorted cells were washed once in PBS and resuspended in a final volume of 15×106 cells/mL. Mice received subcutaneous injections of 200μL (3×106 cells) in each flank (n=6, pcDNA3-GFP; n=6, pcDNA3-ETS1-DN-IRES-GFP). Tumor volume was measured using calipers, with volume calculated as (length × width2) × (π/6).
Kubic J.D., Little E.C., Lui J.W., Iizuka T, & Lang D. (2014). PAX3 and ETS1 synergistically activate MET expression in melanoma cells. Oncogene, 34(38), 4964-4974.
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5

Combination Therapy for MDA-MB-231 Tumor Xenografts

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Athymic nu/nu mice (Charles River Laboratories Inc., Wilmington, MA, USA) were maintained following the Institutional Animal Care and Use Committee (IACUC) procedures and guidelines. 5 × 105 MDA-MB-231 cells were injected subcutaneously in the right flanks of 6 weeks old female athymic nude mice. After one week, tumor-bearing mice (tumor volume around 80 mm3) were randomized into four treatment groups (n = 5) and treated as follows: (1) vehicle (40% β-cyclodextrin in ddH2O, IP), (2) gefitinib (40 mg/kg, IP, once daily), (3) panobinostat(5 mg/kg, IP, once daily) and (4) gefitinib+Pano(G 40 mg/kg+P 5 mg/kg, IP, once daily) 6 days/week for 3 weeks. Tumor volume was calculated by the formula: Length × Width2 /2. At the end of the experiment, mice were sacrificed, and tumors were dissected for further experiments.
Lyu H., Hou D., Liu H., Ruan S., Tan C., Wu J., Hicks C, & Liu B. (2023). HER3 targeting augments the efficacy of panobinostat in claudin-low triple-negative breast cancer cells. NPJ Precision Oncology, 7, 72.
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6

Xenograft Tumor Development and Analysis

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J82-Ras-Lux cells were mixed with Matrigel basement membrane matrix (BD Biosciences, San Jose, CA, USA) and injected subcutaneously into 5-week-old female athymic nu/nu mice (Charles River, Wilmington, MA, USA). Mice were placed in an anesthetic chamber and imaged with the IVIS Lumina system; auto-bioluminescent intensity of inoculated cells was measured photons per second (ph/s). Bioluminescent images were analyzed using Living Image 4.3.1 software (Perkin Elmer). After euthanasia of mice by exposure to CO2, xenograft tumors were harvested, fixed in neutral-buffered formalin, and embedded in paraffin, followed by hematoxylin and eosin (H&E) staining of tissue sections for histopathological examination. All animal procedures were approved by the University of Tennessee Animal Care and Use Committee and were in accordance with the NIH Guide for the Care and Use of Laboratory Animals.
John B.A., Xu T., Ripp S, & Wang H.C. (2017). A Real-Time Non-invasive Auto-bioluminescent Urinary Bladder Cancer Xenograft Model. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 19(1), 10-14.
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7

Orthotopic Pancreatic Cancer Xenograft Model

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All animal procedures were approved by the Pennsylvania State University and Messiah University Institutional Animal Care and Use Committees (IACUCs). The PSU College of Medicine Animal Resource Program is accredited by the Association for Assessment and Accreditation of Laboratory Care International (AAALAC International). All animals’ living conditions were consistent with the standards required by AAALAC International. Four- to six-week-old male athymic (nu/nu) mice were purchased from Charles River. Each xenograft experiment had at least 6 mice per treatment group and was replicated at least twice for rigor and reproducibility. To establish orthotopic pancreatic cancer xenografts, mice were fully anesthetized, a small incision was made in the left flank, the peritoneum was dissected, and the pancreas was exposed. Tumor cells (1 × 106 cells in 100 µL of Hank’s balanced salt solution; HBSS) were injected into the pancreas, and the surgical site was closed with staples.
Abraham T., Armold M., McGovern C., Harms J.F., Darok M.C., Gigliotti C., Adair B., Gray J.L., Kelly D.F., Adair J.H, & Matters G.L. (2024). CCK Receptor Inhibition Reduces Pancreatic Tumor Fibrosis and Promotes Nanoparticle Delivery. Biomedicines, 12(5), 1024.
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8

Metformin's Effect on Xenograft Tumor Growth

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Athymic nu/nu mice (Charles River Laboratories, Wilmington, MA, USA) were maintained in accordance with the Institutional Animal Care and Use Committee (IACUC) procedures and guidelines. Two × 106 H460 were suspended in 100 μL of PBS, mixed with Matrigel (BD Biosciences), and injected subcutaneously into the right flank of female athymic mice. Tumor volume and mouse body weight were measured every other day. The tumor volume was calculated by the formula: volume = (length × width2)/2, where length was the longest axis and width the measurement at a right angle to the length. When tumors reached ∼100 mm3, mice were randomly assigned into two groups (n = 5) and treated daily with sterile water (control) or 350 mg/kg of metformin by oral gavage for 14 days. The tumor growth curves were plotted using average tumor volume and followed by statistical analysis, as we described previously.56 (link),59 (link),60 (link) At the end of treatment, mice were sacrificed and imaged; the tumors were dissected and measured for weight. All tumors and mouse serum were collected for further analysis.
Liu S., Polsdofer E.V., Zhou L., Ruan S., Lyu H., Hou D., Liu H., Thor A.D., He Z, & Liu B. (2021). Upregulation of endogenous TRAIL-elicited apoptosis is essential for metformin-mediated antitumor activity against TNBC and NSCLC. Molecular Therapy Oncolytics, 21, 303-314.
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9

Genetically Engineered Mouse Models for Pancreatic Cancer

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Trp53+/LSL-R172H, Kras+/LSL-G12D and Pdx1-Cre strains in C57Bl/6 background were interbred to obtain Pdx1-Cre; Kras+/LSL-G12D (KC) and Pdx1-Cre; Kras+/LSL-G12D; Trp53+/LSL-R172H (KPC) mice (Hingorani et al., 2005 (link)). The R26LSL-YFP strain was interbred to get the desired genotype. C57Bl/6 and athymic Nu/Nu mice were purchased from Charles River Laboratory and Jackson Laboratory. All animal experiments were conducted in accordance with procedures approved by the IACUC at Cold Spring Harbor Laboratory (CSHL).
Boj S.F., Hwang C.I., Baker L.A., Chio I.I., Engle D.D., Corbo V., Jager M., Ponz-Sarvise M., Tiriac H., Spector M.S., Gracanin A., Oni T., Yu K.H., van Boxtel R., Huch M., Rivera K.D., Wilson J.P., Feigin M.E., Öhlund D., Handly-Santana A., Ardito-Abraham C.M., Ludwig M., Elyada E., Alagesan B., Biffi G., Yordanov G.N., Delcuze B., Creighton B., Wright K., Park Y., Morsink F.H., Molenaar I.Q., Borel Rinkes I.H., Cuppen E., Hao Y., Jin Y., Nijman I.J., Iacobuzio-Donahue C., Leach S.D., Pappin D.J., Hammell M., Klimstra D.S., Basturk O., Hruban R.H., Offerhaus G.J., Vries R.G., Clevers H, & Tuveson D.A. (2014). Organoid Models of Human and Mouse Ductal Pancreatic Cancer. Cell, 160(0), 324-338.
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10

Genetic Mouse Models of Breast Cancer

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Ccr2−/−, Batf3−/−, and OT-1 mice (all on a C57BL/6 background) were obtained from Jackson Laboratory, and athymic (Nu/Nu) mice were obtained from Charles River Laboratory. MMTV-PyMT mice (on C57BL/6 [referred to as BL/6] background) were bred at Cold Spring Harbor Laboratory (CSHL), and MMTV-PyMT-chOVA mice (Engelhardt et al., 2012 (link)) were a kind gift from Dr. Mathew Krummel (University of California, San Francisco, San Francisco, CA). MMTV-PyMT and PyMT-chOVA mice were intercrossed with Ccr2−/− mice. MMTV-PyMT;Ccr2+/+ and MMTV-PyMT;Ccr2−/− mice, and similarly MMTV-PyMT-chOVA;Ccr2+/+ and MMTV-PyMT-chOVA;Ccr2−/− mice, were from the same mouse colony (mice were littermates, or their parents were littermates). All the BL/6 host mice were purchased from Jackson Laboratory. All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at CSHL and were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Fein M.R., He X.Y., Almeida A.S., Bružas E., Pommier A., Yan R., Eberhardt A., Fearon D.T., Van Aelst L., Wilkinson J.E., dos Santos C.O, & Egeblad M. (2020). Cancer cell CCR2 orchestrates suppression of the adaptive immune response. The Journal of Experimental Medicine, 217(10), e20181551.
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