To achieve the attribution of the fluorescence spectral regions, phenolic compounds standards (98–99% purities), including methyl syringate, caffeic, ferulic, syringic, and vanillic acid were purchased from Sigma-Aldrich (Steinheim, Germany). Also, kaempferol, catechin, and phenolic acids (sinapic, gentisic, ellagic, chlorogenic, homogentisic, p-coumaric, protocatechuic, gallic) were purchased from Extrasynthese (Geney, France). Standards were dissolved in MS-grade acetonitrile solution (0.01 mg/mL) (Sigma- Aldrch Merck KGaA, Darmstadt, Germany).
Methyl syringate
Manufactured by Merck Group
Sourced in Germany
Methyl syringate is a chemical compound commonly used as a laboratory reagent. It is a crystalline solid with a characteristic odor. Methyl syringate is a derivative of syringic acid, and its primary function is as a chemical intermediate in various laboratory procedures. The specific details of its intended uses and applications should be obtained from technical literature or expert sources.
Automatically generated - may contain errors
Lab products found in correlation
Vanillic acid, by Merck Group (3 mentions)
Caffeic, by Merck Group (2 mentions)
Ferulic, by Merck Group (2 mentions)
Syringic, by Merck Group (2 mentions)
Sinapic, by Extrasynthese (2 mentions)
Gentisic, by Extrasynthese (2 mentions)
Ellagic, by Extrasynthese (2 mentions)
Chlorogenic, by Extrasynthese (2 mentions)
Homogentisic, by Extrasynthese (2 mentions)
Protocatechuic, by Extrasynthese (2 mentions)
Gallic, by Extrasynthese (2 mentions)
Kaempferol, by Extrasynthese (2 mentions)
Catechin, by Extrasynthese (2 mentions)
P coumaric, by Extrasynthese (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Quercetin, by Merck Group (2 mentions)
Kaempferol, by Merck Group (2 mentions)
Catechin, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
6 protocols using methyl syringate
1
Fluorescence Spectral Analysis of Phenolic Compounds
2
Fungal Laccase Production and Characterization
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Aflatoxin B1 (AFB1), zearalenone (ZEN), deoxynivalenol (DON), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,6-dimethoxy phenol (DMP), syringaldazine (SGZ), and methyl syringate were purchased from Sigma-Aldrich (St. Louis, MO, USA). FB1 (fumonisin B1) and OTA (ocharatoxin A) were purchased from Pribolab (Beijing, China). DNA polymerase, T4 ligase, acetonitrile, and trifluoroacetic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Vanillin, p-coumaric, syringic acid, syringaldehyde, caffeic acid, 1-hydroxybenzotriazole (HBT), gallic acid, isopropyl-β-D-thiogalactoside (IPTG), and kanamycin were purchased from Solarbio (Beijing, China). Ni-NTA agarose was purchased from QIAGEN (Hilden, Germany). The fungal laccase from Ganoderma sp. was purchased from Sunson (Yinchuan, Ningxia, China). Plant extracts from E. brevicornu, C. sativus L., L. angustifolia, A. officinalis, and S. tenuifolia were purchased from Ciyuan Biotech (Xi’an, Shanxi, China). All other chemicals were of analytical grade or chromatographically pure, and were commercially available.
3
Quantification of Polyphenols in Honey
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Gallic acid, protocatechuic acid, catechin, 4-hydroxybenzoic acid, chlorogenic acid, caffeic acid, phenyllactic acid, 2,4- dihydroxybenzoic acid, vanillic acid, syringic acid, p-caumaric acid, ferulic acid, rutin, sinapic acid, cinnamic acid, rosmarinic acid, methyl syringate, quercetin, hesperetin and kaempferol were obtained from Sigma Aldrich (Australia). HPLC grade methanol was supplied by Merck (Australia). Stock solutions of the compounds were prepared by dissolving 5 mg in methanol. Phenomenex (Australia) Strata-X SPE cartridges (500 mg/6 ml, surface area 800 m2 g−1, particle size 33 μm, average pore size 85 Å) were used to pre-concentrate the target analytes. SPE cartridges were conditioned according to the manufacturer's instructions before loading the honey samples.
4
Silylation and Anticancer Assays Protocol
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The silylation reagent N,O-Bis(trimethylsilyl)trifluoroacetamid (BSTFA) with 1% trimethylchlorosilane (TMSC), pyridine, dimethyl sulfoxide (DMSO), hexane, methylthiazolyldiphenyltetrazolium bromide (MTT), methyl syringate applied as internal standard (IS), pinobanksin, pinocembrin, p-coumaric acid, caffeic acid, ferulic acid, formaldehyde solution, albumin bovine serum (BSA), Triton™-100 were obtained from Sigma-Aldrich (Steinheim, Germany). Methanol for GC was purchased from POCh (Gliwice, Poland). Chrysin and galangin were purchased from Roth (Karlssruhe, Gremany). Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS), phosphate buffered saline (PBS) and pencyllin-streptomycin (10,000 U/mL) were products of Gibco (Waltham, MA, USA). Primary antibodies anti caspase-3 (#559565), caspase-8 (#51-80851-N) and caspase-9 (#51-80861N), FITC Fluor-conjugated secondary antibody, (FITC goat anti-mouse IgG #554001, FITC goat anti-rabbit IgG #554020) were obtained from Becton Dickinson (New Jersey, USA). Hoechst 33342 (#561908) was product of ThermoFisher Scientific (USA). All chemicals and reagents used in this study were of analytical grade.
5
Cellular Antioxidant Assay Protocol
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2,7-dichlorofluorescein diacetate (DCFH-DA), 2,2′-Azo-bis-aminopropane (ABAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hanks' Balanced Salt Solution (HBSS), fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM), and other cell culture reagents were purchased from Gibco (Invitrogen, Barcelona, Spain). Methyl syringate, epicatechin, syringic acid, catechin, 4-coumaric acid, gallic acid, quercetin, apigenin, luteolin, kaempferol, naringenin, formononetin, genistein, 3-coumaric acid, daidzein, pelargonidin, 2-coumaric acid, biochanin A and cyanidin were purchased from Sigma. Ultrapure water was obtained using a Milli-Q system (Millipore, Bedford, MA, USA) and methanol, acetonitrile and 2-propanol were purchased from Teknokroma (Barcelona, Spain). All other chemicals were of analytical grade.
6
Quantification of Phenolic Compounds
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Standards of phenolics, including kaempferol, catechin, chrysin, hesperetin, isorhamentin, naringenin, and phenolic acids (gallic, chlorogenic, ellagic, gentisic, homogentisic, pcoumaric, protocatechuic, sinapic) were purchased from Extrasynthese (Genay, France). Methyl syringate, caffeic, ferulic, syringic, and vanillic acids were purchased from Sigma-Aldrich (Steinheim, Germany). The purities of the standards were 98-99%. The chemical structures are shown in Table S1. All solutions were prepared by dissolving the abovementioned phenolic compounds in MS-grade acetonitrile (0.01 mg/mL) (Sigma-Aldrich Merck KGaA, Darmstadt, Germany).
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