Formalin-fixed, paraffin-embedded specimens were collected, and a routine H&E slide was first evaluated. Immunohistochemical staining was done on 5 μm-thick paraffin-embedded sections using mouse anti-Merlin (Abcam), rabbit anti-GPX4 (Abcam), rabbit anti-PTGS2 (Cell Signaling), mouse anti-Ki67 (Cell Signaling), rabbit anti-ACSL4 (Thermo Fisher), rabbit anti-TFRC (Abcam), and rabbit anti-YAP (Cell Signaling) antibodies with a standard avidin-biotin HRP detection system according to the instructions of the manufacturer (anti-mouse/rabbit HRP-DAB Cell & Tissue Staining Kit, R&D Systems Minneapolis, MN). Tissues were counterstained with haematoxylin, dehydrated, and mounted. In all cases, antigen retrieval was done with the BD Retrievagen Antigen Retrieval Systems as per instructions of the manufacturer.
Rabbit anti acsl4
Manufactured by Thermo Fisher Scientific
Rabbit anti-ACSL4 is a primary antibody that specifically targets the ACSL4 (Acyl-CoA Synthetase Long Chain Family Member 4) protein. ACSL4 is an enzyme involved in the activation of long-chain fatty acids. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunocytochemistry to detect and study the ACSL4 protein.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti merlin, by Abcam (2 mentions)
Rabbit anti gpx4, by Abcam (2 mentions)
Rabbit anti ptgs2, by Cell Signaling Technology (2 mentions)
Mouse anti ki67, by Cell Signaling Technology (2 mentions)
Rabbit anti tfrc, by Abcam (2 mentions)
Anti mouse rabbit hrp dab cell tissue staining kit, by R&D Systems (2 mentions)
Rabbit anti yap, by Cell Signaling Technology (2 mentions)
2 protocols using rabbit anti acsl4
1
Immunohistochemical Analysis of Formalin-Fixed Tissues
2
Immunohistochemical Analysis of Formalin-Fixed Tissues
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