Analytical standard of metronidazole (MET) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Two types of titanium(IV) oxide were used in this study—namely Degussa P25 (from Evonik, Essen, Germany) and TiO2 in the form of anatase from Sigma-Aldrich (St. Louis, MO, USA). Based on information from the manufacturer, the size of the anatase particles was <25 nm, while the specific surface area ranged from 45 to 55 m2 g−1. In the case of P25, its particle size was about 27 nm, and the specific surface area was at the level 57 m2 g−1. The other semiconductor catalysts such as zinc oxide, tungsten(VI) oxide, zircon (IV) oxide, and lead(II) sulphide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Analytical-grade methanol was purchased from CHEMPUR (Piekary Śląskie, Poland). Hypergrade acetonitrile, formic acid, trifluoroacetic acid, water, and methanol were obtained from Merck (Darmstadt, Germany) and used for the LC analyses.
Acetonitrile hypergrade
Manufactured by Merck Group
Sourced in Germany
Acetonitrile (hypergrade) is a high-purity organic solvent commonly used in analytical chemistry and various laboratory applications. It serves as a mobile phase component in liquid chromatography, a reagent in organic synthesis, and a solvent for extraction and sample preparation. The hypergrade designation indicates the product meets strict quality control standards for enhanced purity and consistency.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (5 mentions)
Methanol, by Merck Group (4 mentions)
Analytical grade methanol, by Chempur (2 mentions)
Formic acid, by Biosolve (2 mentions)
Sml0982, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Vanillic acid, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Zinc oxide, by Merck Group (1 mentions)
Anatase, by Merck Group (1 mentions)
Degussa p25, by Evonik (1 mentions)
Metronidazole met, by Merck Group (1 mentions)
Tungsten 6 oxide, by Merck Group (1 mentions)
Petroleum ether, by Merck Group (1 mentions)
Analytical grade acetone, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Milli q ultrapure water purification system, by Merck Group (1 mentions)
Lecithin e80, by Lipoid (1 mentions)
Oasis hlb cartridge, by Waters Corporation (1 mentions)
15 protocols using acetonitrile hypergrade
1
Photocatalytic Degradation of Metronidazole
2
Astaxanthin Detection and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analytical-grade acetone, petroleum ether and hypergrade acetonitrile were obtained from Merck (Darmstadt, Germany). Ethanol and Tris(hydroxymethyl) aminomethane (TRIS) (≥99.9%) were provided by Carl Roth (Karlsruhe, Germany), and formic acid (99% ULC/MS) by Biosolve (Valkenswaard, The Netherlands). Cholesterol esterase from Pseudomonas sp. was purchased from MP Biomedicals (Eschwege, Germany). All-E-astaxanthin standard in its free form (SML0982, ≥97%, 3S,3′S, from Blakslea trispora) was obtained from Sigma-Aldrich (Taufkirchen, Germany), and astaxanthin monopalmitate (1017, 3RS, 3′RS) was provided by CaroteNature (Münsingen, Switzerland).
3
Synthesis and Characterization of Aripiprazole and Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aripiprazole was obtained from Otsuka pharmaceutics (Tokyo, Japan), while N-hydroxymethyl-aripiprazole and aripiprazole lauroxil were synthesised as described below. Reagents and solvents for the synthetic work were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Viscoleo, Ph. Eur Grade medium chain triglyceride, C8/C10 (MCT) was purchased from Delios V (Illertissen, Germany), lecithin (E80) for the intravenous emulsion was obtained from Lipoid AG (Ludwigshafen, Germany) and glycerol from Sigma Aldrich (St. Louis, MO, USA). Hypergrade acetonitrile from Merck (Darmstadt, Germany) was used for the HPLC–MS/MS analysis, ethanol was from De Danske Spritfabrikker (Aalborg, Denmark) and deuterated aripiprazole used as the internal standard in the bioanalysis was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Purified water was obtained from a Millipore Milli-Q Ultrapure Water purification system (Billerica, MA, USA). All other chemicals were of analytical grade or higher.
4
Quantitative Analysis of Sulfonamide and Trimethoprim
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analytical standards of sulfamethoxazole (SMX) and trimethoprim (TRI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hypergrade acetonitrile, formic acid, and water were purchased from Merck (Darmstadt, Germany). Analytical-grade methanol, hydrochloric acid, sulfuric acid, and disodium phosphate were purchased from CHEMPUR (Piekary Śląskie, Poland). Analytical-grade acetic acid was purchased from POCH S.A. (Gliwice, Poland). Analytical-grade acetonitrile was purchased from Eurochem (Tarnów, Poland). Analytical-grade citric acid, formic acid, and acetic acid were purchased from ChemLand (Stargard, Poland). Analytical-grade sodium hydroxide was purchased from POCH S.A. (Gliwice, Poland). Oasis HLB cartridges of varying masses of sorbent (500 mg, 6 mL; 400 mg, 6 mL; 200 mg, 3 mL) from Waters (Eschborn, Germany) were used for solid-phase extraction.
5
Analysis of Polyphenol Standards
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microfiltered and ultrapure water was used for the preparation of the solutions via the Merck Millipore ZRQS0P3FR Direct system. All solvents and reagents used in the experiments were of a high degree of purity. Ethanol ≥99.9% ACS for analysis, methanol for HPLC, anhydrous 95% n-hexane, chloroform for chromatography, 1-butanol ACS reagent ≥99.5%, hydrochloric acid 37% RPE for analysis, potassium bicarbonate, anhydrous sodium carbonate for analysis, gallic acid ACS for analysis and Sudan IV reagent were purchased from Sigma-Aldrich Chemical Company (Milan, Italy). Folin-Ciocălteu reagent and hypergrade acetonitrile for LC-MS were purchased from Merck Millipore GmbH (Milan, Italy). 2,2-diphenyl-1-picrylhydrazyl (DPPH·) was obtained from Alfa Aesar (from Thermo Fisher Scientific companies in Rodano, Milan, Italy). Polyphenol standards (malvidin-3-O-glucoside, naringin, catechin, quercetin, gallic acid, vanillic acid, caffeic acid, ferulic acid), amino acid standard mixture, methanol, acetic acid, gallic acid, were purchased from Merck (Darmstadt, Germany); 2-propanol and acetonitrile (ACN) from Honeywell (Charlotte, USA), formic acid by J.T. Baker (Rodano, Italy).
6
Quantitative Analysis of Polyphenols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hypergrade acetonitrile (Merck, Darmstadt, Germany) and formic acid (Sigma, Steinheim, Germany) were used for LC-MS. Reference compounds were obtained from Sigma-Aldrich (Steinheim, Germany) ((+)-catechin, caffeic acid, (−)-epicatechin, gallic acid, quercetin 3-rhamnoside, and quercetin 3-rutinoside), Extrasynthese (Lyon, France) (chlorogenic acid and quercetin 3-glucoside), and Phytolab (Vestenbergsgreuth, Germany) (neochlorogenic acid, cryptochlorogenic acid, 3,5-dicaffeoylquinic acid, procyanidin B1 and B2, and procyanidin C1). Ultrapure water (SimplicityTM Water Purification System, Millipore, Marlborough, MA, USA) was used to prepare all solutions.
7
TLC-based Metabolite Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A TLC plate (Glass TLC plate, silica gel coated with fluorescent indicator F254), polyethylene glycol 4000, and hyper-grade acetonitrile for liquid chromatography–mass spectrometry (LC-MS) were obtained from Merck KGaA (Darmstadt, Germany). BD Bacto yeast extract (Lot No. 1186275), hygromycin B, G418 sulfate, NBP, TEPA, TLC solvent, and the other chemicals were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan).
8
Quantitative Analysis of Xanthohumol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol and acetonitrile, HPLC grade, and acetonitrile, hypergrade, for LC-MS were supplied by Merck (Darmstadt, Germany). Formic acid, HPLC grade, purity 50%, was supplied by Fluka analytical (St. Louis, MO, USA). Xanthohumol (CAS number: (6754-58-1), purity 98%) was purchased from Carbosynth (Berkshire, UK). Purified water (Type I) was obtained from an Autwomatic purification system (Wasserlab, Navarra, Spain).
An individual stock solution of xanthohumol was prepared in Methanol at the concentration of 1 mg/mL. Intermediate standard solutions were prepared in Methanol by diluting the stock solution in the concentration range 0.1–20 mg/L; a solution of 0.05 mg/L and 0.016 mg/L were prepared to determine the limits of detection and quantification respectively. Stock and working standard solutions were stored at 4 °C and protected from the light before analysis.
An individual stock solution of xanthohumol was prepared in Methanol at the concentration of 1 mg/mL. Intermediate standard solutions were prepared in Methanol by diluting the stock solution in the concentration range 0.1–20 mg/L; a solution of 0.05 mg/L and 0.016 mg/L were prepared to determine the limits of detection and quantification respectively. Stock and working standard solutions were stored at 4 °C and protected from the light before analysis.
9
Astaxanthin Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analytical grade acetone (SupraSolv), petroleum ether, and acetonitrile (hypergrade) were obtained from Merck (Darmstadt, Germany). Ethanol and Tris(hydroxymethyl)aminomethan (TRIS) (≥ 99.9%) were provided by Carl Roth (Karlsruhe, Germany) and formic acid (99% ULC/MS) by Biosolve (Valkenswaard, Netherlands). Hydrochloric acid for pH value adjustment was purchased from Merck (Darmstadt, Germany). Cholesterol esterase from Pseudomonas sp. for enzymolysis was obtained from MP Biomedicals (Eschwege, Germany). All-E-astaxanthin standard in its free form (SML0982, ≥ 97%, 3S,3’S, from Blakeslea trispora) was provided by Sigma-Aldrich (Taufkirchen, Germany) and astaxanthin monopalmitate (1017, 3RS,3’RS) by CaroteNature (Münsingen, Swiss).
10
Daylily Compound Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dried daylily was purchased from Yonghui Market (Beijing, China). MEthanol and acetonitrile (hypergrade) were purchased from Merck (Darmstadt, Germany). Ethanol was purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Cellulase and pectinase with the enzyme activity of 100,000 U/g were purchased from Pangbo Biological Co. Ltd. (Nanning, China). Ultra-pure water was purchased from Watsons Group Co. Ltd. (Hongkong, China). The kit (G0117F) was purchased from Suzhou Grace Biotechnology Co. Ltd. (Suzhou, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!