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Embryomax ksom embryo culture

Manufactured by Merck Group

EmbryoMax KSOM Embryo Culture is a laboratory product designed for in vitro culture of embryos. It provides a balanced salt solution and energy sources to support embryo development in a controlled environment.

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2 protocols using embryomax ksom embryo culture

1

Embryo Injection and Culture

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During the process of injection, mouse 8-cell embryos were placed in drops of EmbryoMax M2 medium (Millipore, MR-015-D) covered with Mineral oil (Sigma-Aldrich, M8691). After injection, the embryos were transferred into drops of EmbryoMax KSOM Embryo Culture (Millipore, MR-020P-5F) or G-2 PLUS medium (Vitrolife, 10132) in a humidified incubator under 5% CO2 at 37°C.
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2

Mouse Embryo Culture and Microinjection

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For embryo culture, mouse embryos were kept in 20 µL drops of EmbryoMax KSOM embryo culture (Millipore, MR-020P-5F) covered with mineral oil (Sigma-Aldrich, M8691) in a humidified incubator under 5% CO2 at 37 °C. During the process of microinjection, embryos were placed in 10 µL EmbryoMax M2 medium (Millipore, MR-015-D) covered with mineral oil.
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