RBD and NP end-point titers were determined using standard ELISA and plates coated with 500 ng/mL SARS-CoV-2 Spike-protein Receptor-Binding Domain (RBD) (GenScript, Piscataway NJ) or 1ug/mL SARS-CoV-2 nucleocapsid protein (NP). Heat inactivated plasma was added in 3-fold dilutions starting at 1:50. Responses were visualized by adding HRP-anti-human IgG (BD Pharmingen) followed by the addition of Phenylenediamine dihydrochloride (ThermoFisher Scientific). ODs were read at 490 nm on a Victor3™ Multilabel plate reader (Perkin Elmer). Batch differences were minimized by normalizing to positive control samples run on each plate.
Sars cov 2 spike protein receptor binding domain
Manufactured by GenScript
The SARS-CoV-2 Spike-protein Receptor-Binding Domain (RBD) is a recombinant protein that represents the receptor-binding domain of the SARS-CoV-2 spike protein. The RBD is responsible for the initial attachment of the virus to the host cell receptor, ACE2, during the infection process.
Automatically generated - may contain errors
2 protocols using sars cov 2 spike protein receptor binding domain
1
SARS-CoV-2 Antibody Quantification ELISA
2
SARS-CoV-2 Antibody Endpoint Titers
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RBD and NP end-point titers were determined using standard ELISA and plates coated with 500 ng/mL SARS-CoV-2 Spike-protein Receptor-Binding Domain (RBD) (GenScript, Piscataway NJ) or 1ug/mL SARS-CoV-2 nucleocapsid protein (NP). Heat inactivated plasma was added in 3-fold dilutions starting at 1:50. Responses were visualized by adding HRP-anti-human IgG (BD Pharmingen) followed by the addition of o-phenylenediamine dihydrochloride (ThermoFisher Scientific). ODs were read at 490 nm on a Victor3™ Multilabel plate reader (Perkin Elmer). Batch differences were minimized by normalizing to positive control samples run on each plate.
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