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Trzol

Manufactured by Thermo Fisher Scientific
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Sourced in United States

TRIzol is a reagent used for the isolation and purification of total RNA from various biological samples, including cells, tissues, and bodily fluids. It is a monophasic solution containing phenol and guanidinium isothiocyanate, which facilitates the lysis of cells and the separation of RNA from other cellular components. TRIzol is a versatile and effective tool for RNA extraction, providing high-quality, pure RNA for various downstream applications, such as RT-PCR, Northern blotting, and gene expression analysis.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Anti β tubulin, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin combination, by Thermo Fisher Scientific (2 mentions) Protein lysis buffer, by Solarbio (2 mentions) Dmem medium, by Thermo Fisher Scientific (2 mentions) Anti α7nachr, by Abcam (2 mentions) Anti phospho p65 nf κb 3033s, by Cell Signaling Technology (2 mentions) Alexafluor488 mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexafluor488 rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions) Reverse transcription master mix kit, by Thermo Fisher Scientific (2 mentions) Anti phospho iκb, by Cell Signaling Technology (2 mentions) Sybr select master mix, by Thermo Fisher Scientific (1 mentions) Anti il 1β, by Abcam (1 mentions) Anti iκb, by Cell Signaling Technology (1 mentions) Anti cd68, by Abcam (1 mentions)

Spelling variants of this product

TRZOL reagent (5 citations) TRZol reagent extraction kit (2 citations)

2 protocols using trzol

1

Molecular Mechanisms of Microglial Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tris, sodium dodecyl sulfate (SDS), 30% acrylamide and protein lysis buffer (#R0010) were obtained from Solarbio Science & Technology Company (Beijing, China). TRzol was purchased from Ambion company (Texas, United States). Anti-CD11b (#ab1211, #ab133357), anti-IL-1β (#ab9787), anti-CD40 (#ab13545), anti-CD68 (#ab125212), and anti-α7nAChR (#ab10096) were purchased from Abcam (Cambridge, United Kingdom). Anti-IKK (#2682s), anti-phospho-IKK(#2697s), anti-P65-NF-κB (#242s), anti-phospho-P65--NF-κB (#3033s), anti-IκB (#4814s) and anti-phospho-IκB (#9246s) were purchased from Cell Signaling Technology (Massachusetts, United States). Anti-β-tubulin (#MA511732), AlexaFluor488 mouse secondary antibody (#A11029), AlexaFluor488 rabbit secondary antibody (#A11034), enhanced chemiluminescence reagent (ECL) Western blotting detection reagents (#32106), Reverse Transcription Master Mix kit (Invitrogen, #4374966), SYBR® Select Master Mix (Life Technologies, #4472908), 0.25% trypsin (Gibco, #25200-056), DMEM medium (Gibco, #31800-014), fetal bovine serum (FBS) (Gibco, #10099-141) and penicillin streptomycin combination (Gibco, #15140-122) were purchased from Thermo Fisher Scientific (Massachusetts, United States). 2-, 3-, 5-triphenyltetrazolium chloride (TTC, #T8877-25G) was purchased from Sigma (United States).
Liu C., Liu S., Xiong L., Zhang L., Li X., Cao X., Xue J., Li L., Huang C, & Huang Z. (2021). Genistein-3′-sodium sulfonate Attenuates Neuroinflammation in Stroke Rats by Down-Regulating Microglial M1 Polarization through α7nAChR-NF-κB Signaling Pathway. International Journal of Biological Sciences, 17(4), 1088-1100.
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2

Molecular Mechanisms of Neuroinflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tris, sodium dodecyl sulfate (SDS), 30% acrylamide and protein lysis buffer (#R0010) were obtained from Solarbio Science & Technology Company (Beijing, China). TRzol was purchsed from Ambion company (Texas, United States). Anti-CD11b (#ab1211), anti-IL-1β (#ab9787), anti-CD40 (#ab13545), anti-CD68 (#ab31630), and anti-α7nAChR (#ab10096) were purchased from Abcam (Cambridge, United Kingdom). Anti-IKK (#2682s), anti-phospho-IKK(#2697s), anti-P65-NF-κB (#242s), anti-phospho-P65--NF-κB (#3033s), anti-IκB(#4814s) and anti-phospho-IκB (#9246s) were purchased from Cell Signaling Technology (Massachusetts, United States). Anti-β-tubulin (#MA511732), AlexaFluor488 mouse secondary antibody (#A11029), AlexaFluor488 rabbit secondary antibody (#A11034), enhanced chemiluminescence reagent (ECL) Western blotting detection reagents (#32106), Reverse Transcription Master Mix kit (Invitrogen, #4374966), SYBR Ò Select Master Mix (Life Technologies, #4472908), 0.25% trypsin (Gibco, #25200-056), DMEM medium (Gibco, #31800-014), fetal bovine serum (FBS) (Gibco, #10099-141) and penicillin streptomycin combination (Gibco, #15140-122) were purchased from Thermo Fisher Scienti c (Massachusetts, United States). 2-, 3-, 5-triphenyltetrazolium chloride (TTC, #T8877-25G) was purchased from Sigma (United States).
Liu C., Liu S., Xiong L., Li X., Cao X., Xue J., Li L., Huang C., & Huang Z. (2020). Genistein-3′-sodium Sulfonate Attenuates Cerebral Ischemia/reperfusion Injury in Rats by Down-regulating Microglial M1 Polarization Through α7nAChR-mediated Inhibition of NF-κb Signaling Pathway .
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