Pgp aav syn jgcamp7f wpre

Viral injections were performed as described previously (Leroy et al., 2017 (link), 2018 (link)). Briefly, mice were anesthetized with isoflurane and placed in a stereotaxic apparatus. A craniotomy was performed above the target region and a glass micropipette was used for viral injection. Injections were performed using a nano-inject II (Drummond Scientific). Twenty-three nl of solution were delivered every 15 s until the total amount was reached. The micropipette was retracted after 5 min. We bilaterally injected 368 nl of AAV2/9 hSyn.hChR2(H134R).eYFP.WPRE.hGH (UPenn Vector Core, titer 3.6*10¹² genome copy (GC) ml⁻¹) or AAV2/9 CaMKII.ArchT-GFP (UNC Vector Core, viral titer 2.2*10¹² GC ml⁻¹) or pGP-AAV-syn-jGCaMP7f-WPRE (Addgene, titer 3.6*10¹² GC ml⁻¹) or AAV2/9-CaMKIIa-hM4D(Gi)-mCherry (Addgene, titer 2.5*10¹² GC ml⁻¹) to the LEC or MEC. The positions were: −3.4 mm AP, +/− 4.7 mm ML and 2.8 mm DV for LEC injections and −4.9 mm, +/− 3.4 mm ML and 2.8 mm DV for MEC injections. Mice were allowed to recover for 2–3 weeks.