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Rea isotype control

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The REA Isotype Control is a laboratory reagent used to establish background staining levels in flow cytometry experiments. It is designed to match the isotype and conjugate of a specific antibody, allowing researchers to distinguish specific antibody binding from non-specific background staining.

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Lab products found in correlation

Cd151, by Miltenyi Biotec (2 mentions) Iggk2b, by Miltenyi Biotec (2 mentions) Cd151, by BioLegend (2 mentions) Iggk1, by BioLegend (2 mentions) Epcam, by Miltenyi Biotec (2 mentions) Epcam, by BioLegend (2 mentions)

2 protocols using rea isotype control

1

Liver Cancer EV Subpopulation Isolation

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the following antibodies were used at a concentration of 1 μg Ab/mL sample: EpCAM (Biolegend), CD151 (Miltenyi), and EGFR (Thermo Fisher). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151), and the Biolegend IgGK1 (to match EGFR). Notably, the liver cancer EV subpopulation isolation required filtering with a .45 μm filter unit (GE Whatman) to achieve strong specificity versus isotype control. Samples were run at a flow rate of = 2.5 mL/hr.
Lin A.A., Shen H., Spychalski G., Carpenter E.L, & Issadore D. (2023). Parallelized immunomagnetic nanopore sorting: modeling, scaling, and optimization of surface marker specific isolation of extracellular vesicles from complex media. Research Square.
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2

Liver Cancer EV Subpopulation Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used at a concentration of 1 µg Ab/mL sample: EpCAM (Biolegend), CD151 (Miltenyi), and EGFR (Thermo Fisher). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151), and the Biolegend IgGK1 (to match EGFR). Notably, the liver cancer EV subpopulation isolation required filtering with a 0.45 µm filter unit (GE Whatman) to achieve strong specificity versus isotype control. Samples were run at a flow rate of ɸ = 2.5 mL/h.
Lin A.A., Shen H., Spychalski G., Carpenter E.L, & Issadore D. (2023). Modeling and optimization of parallelized immunomagnetic nanopore sorting for surface marker specific isolation of extracellular vesicles from complex media. Scientific Reports, 13, 13292.
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