Human il 6 platinum elisa

Manufactured by Thermo Fisher Scientific

Sourced in France, Australia

The Human IL-6 Platinum ELISA is a laboratory assay used to quantitatively measure the concentration of interleukin-6 (IL-6) in human biological samples. It is an Enzyme-Linked Immunosorbent Assay (ELISA) that employs the quantitative sandwich enzyme immunoassay technique.

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Spelling variants of this product

Human IL-6 (69 citations) ELISAs (35 citations) Human IL-6 platinum ELISA kit (3 citations) Human IL-6 Platinum Ready-to-use Sandwich ELISA (2 citations) Human IL-6 and IL-18 Platinum ELISA kits (2 citations)

6 protocols using human il 6 platinum elisa

Quantifying HIV Reservoirs and Biomarkers

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HIV-RNA in blood plasma was quantified locally at all study-visits, by real-time PCR (Roche or Abbott) as described above for the semen.
Blood samples were centralized for total cell-associated HIV-DNA quantification. Thawed whole blood was analyzed with the same ultrasensitive real-time PCR method as described above for the semen (Generic HIV-DNA assay, Biocentric, Bandol, France) [20 (link)]. Each entire DNA extract (quantified with Nanodrop as previously described) was tested in two replicates, and the results were reported as the number of HIV-DNA copies per 10⁶ PBMC, taking into account the whole blood cell count.
Frozen blood plasma samples from the biobank were addressed to the Paris Pasteur Institute and Inserm U1184. Levels of IL-6, IP-10, sCD14 and sCD163 were measured in duplicate with specific ELISA assays (Human IL-6 Platinum ELISA, eBioscience; Human quantikine CXCL10 ELISA, R&D ELISA R&D; Human CD14 DuoSet ELISA and Human CD163 DuoSet ELISA, R&D Systems, Minneapolis, Minnesota). Samples with undetectable levels of a given analyte were arbitrarily attributed half the minimal detectable value.

Chéret A., Durier C., Mélard A., Ploquin M., Heitzmann J., Lécuroux C., Avettand-Fenoël V., David L., Pialoux G., Chennebault J.M., Müller-Trutwin M., Goujard C., Rouzioux C, & Meyer L. (2017). Impact of early cART on HIV blood and semen compartments at the time of primary infection. PLoS ONE, 12(7), e0180191.

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Quantification of IP-10 and IL-6

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IP-10 concentrations were determined in stored plasma or serum samples (−80°C) by specific enzyme-linked immunosorbent assay, human Quantikine CXCL10 (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions. Levels of IL-6 were measured in frozen plasma samples with specific ELISA assays (Human IL-6 Platinum ELISA, eBioscience). Samples with undetectable levels of IL-6 were arbitrarily attributed half the minimal detectable value (0.46 pg/mL).

Gondois-Rey F., Chéret A., Mallet F., Bidaut G., Granjeaud S., Lécuroux C., Ploquin M., Müller-Trutwin M., Rouzioux C., Avettand-Fenoël V., De Maria A., Pialoux G., Goujard C., Meyer L, & Olive D. (2017). A Mature NK Profile at the Time of HIV Primary Infection Is Associated with an Early Response to cART. Frontiers in Immunology, 8, 54.

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Quantification of Plasma Cytokines

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IP-10 concentrations were determined in stored (−80 °C) plasma samples by human Quantikine CXCL10 ELISA assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions. Levels of IL-6 were measured on plasma frozen samples with Human IL-6 Platinum ELISA (eBioscience, Paris, France). Samples with undetectable levels of IL-6 were attributed half the minimal detectable value (0.46 pg ml⁻¹).

Gondois-Rey F., Chéret A., Granjeaud S., Mallet F., Bidaut G., Lécuroux C., Ploquin M., Müller-Trutwin M., Rouzioux C., Avettand-Fenoël V., Moretta A., Pialoux G., Goujard C., Meyer L, & Olive D. (2017). NKG2C+ memory-like NK cells contribute to the control of HIV viremia during primary infection: Optiprim-ANRS 147. Clinical & Translational Immunology, 6(7), e150-.

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Nerve Function and Inflammatory Markers

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Nerve conduction studies (NCVs)

Electrophysiological studies were carried out unilaterally (right side) under a uniform protocol using a Nicolet/VIASYS Viking Quest EMG Machine [16 ]. Thirty-two to 34^°C was set as the distal skin temperature. The estimated parameters included: Distal motor latency (DML), peak to baseline amplitude of compound muscle action potential (a-CMAP), and motor conduction velocity for tibial, Peroneal and ulnar nerves. Moreover, peak-to-peak amplitude of sensory action potentials (a-SAP) and sensory conduction velocity (antidromic technique) of sural and ulnar nerves were also measured.

Proinflammatory cytokines

Serum levels of proinflammatory cytokines: interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and high-sensitivity C-reactive Protein (hs-CRP) were measured before the onset of chemotherapy and one month after the cessation of treatment by Human IL-6 Platinum ELISA, Human TNF alpha Platinum ELISA (eBioscience, INC. San Diego, CA 92121, USA) and Minineph™ Human kits (Birmingham, UK) respevtively.

Esfahani A., Somi M.H., Ayromlou H., Nikanfar A., Jafarabadi M.A., Sadat B.E, & Ghoreishi Z. (2016). The effect of n-3 polyunsaturated fatty acids on incidence and severity of oxaliplatin induced peripheral neuropathy: a randomized controlled trial. Biomarker Research, 4, 13.

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Comprehensive Blood Analyte Profiling

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The human blood samples were analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and bilirubin and conjugated bilirubin by using routine hospital laboratory methods. Serum total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were determined enzymatically. The plasma biochemistries for all animals were analyzed with a CHEMIX-180 multiple analyzer (Sysmex Corporation, Japan) as previously described [12 (link)]. For ELISA analysis, the human IL-6 Platinum ELISA (BMS213, eBioscience, Waltham, MA) Kit, human TNF-alpha Platinum ELISA (BMS223, eBioscience, Waltham, MA) Kit and human Neurotensin ELISA Kit (CSB-E09144h, Cusabio, Wuhan, China) were used in this study according to protocols of manufactures.

Xiao Y., Yan W., Lu Y., Zhou K, & Cai W. (2018). Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake. EBioMedicine, 35, 133-141.

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Quantification of Inflammatory Mediators

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To evaluate the secretion of IL-6, IL-8 and Angiotensin II, cellular supernatant was collected subsequent to each treatment, aliquoted into eppendorf tubes, and stored at −80°C. IL-6, IL-8 and Angiotensin II concentrations were quantified using commercially available ELISA kits (Human IL-6 Platinum ELISA: BMS213/2, eBioscience, Tasmania, Australia; Human IL-8 Platinum ELISA: BMS204/3, eBioscience; Angiotensin II ELISA kit: ADI-900–204, Enzo Life Sciences, Farmingdale, USA; Bradykinin ELISA kit: ADI-900–206, Enzo Life Sciences) according to procedures recommended by the manufacturer.(5 (link), 26 (link))

Fu Z., Wang F., Liu X., Hu J., Su J., Lu X., Lu A., Cho J.M., Symons J.D., Zou C.J, & Yang T. (2021). Soluble (Pro)Renin Receptor Induces Endothelial Dysfunction and Hypertension in Mice with Diet-Induced Obesity via Activation of Angiotensin II Type 1 Receptor. Clinical science (London, England : 1979), 135(6), 793-810.

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