Paraffin embedded prostate cancer tissue blocks were from the Vancouver Prostate Centre Tissue Bank. Patient consent was reviewed and approved by the University of British Columbia Clinical Research Ethics Board (certificate no. H09-01628). Immunohistochemical staining was conducted as previously described 56 (link) using the Ventana DiscoverXT Autostainer (Ventana Medical System) with enzyme labeled biotin streptavidin system and solvent-resistant DAB Map kit. Antibodies used in IHC include anti-FoxA1 (Abcam; ab23738), anti-SYP (Abcam; ab32127), anti-chromogranin A (Millipore; MAB5268), and anti-IL-8 (R&D systems).
Ventana discoverxt autostainer
Manufactured by Roche
The Ventana DiscoverXT Autostainer is a laboratory equipment product designed for automated immunohistochemistry (IHC) and in situ hybridization (ISH) staining. It is used to process tissue samples and perform various staining protocols in a controlled and consistent manner.
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Lab products found in correlation
3 protocols using ventana discoverxt autostainer
1
Prostate Cancer Tissue Immunohistochemistry
2
HER2 Immunohistochemistry in Tumors
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Immunohistochemical staining was conducted using the Ventana Discover XT Autostainer (Ventana Medical System) on paraffin embedded tumors with enzyme labeled biotin streptavidin system and a solvent-resistant DAB Map kit by using HER2 antibody. Slides were scored as follows: 0= no staining, 1= faint or focal stain, 2= convincing intensity in a minority of cells, and 3= convincing intensity in a majority of cells. The overall percentage of cancer cells showing staining (0%–100%) was also indicated. Scoring was conducted at 200x.
3
Prostate Cancer Tissue Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin embedded prostate cancer tissue blocks were from the Vancouver Prostate Centre Tissue Bank. Patient consent was reviewed and approved by the University of British Columbia Clinical Research Ethics Board (certificate no. H09-01628). Immunohistochemical staining was conducted as previously described 56 (link) using the Ventana DiscoverXT Autostainer (Ventana Medical System) with enzyme labeled biotin streptavidin system and solvent-resistant DAB Map kit. Antibodies used in IHC include anti-FoxA1 (Abcam; ab23738), anti-SYP (Abcam; ab32127), anti-chromogranin A (Millipore; MAB5268), and anti-IL-8 (R&D systems).
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