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Pfn21a halotag cmv flexi vector

Manufactured by Promega
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Sourced in United States

The PFN21A HaloTag CMV Flexi Vector is a laboratory tool designed for the expression and purification of recombinant proteins. It contains a HaloTag coding sequence, which allows for the covalent attachment of HaloTag ligands, facilitating protein detection and purification. The vector utilizes the Cytomegalovirus (CMV) promoter for high-level protein expression in mammalian cell systems.

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Lab products found in correlation

Neon transfection system, by Thermo Fisher Scientific (1 mentions) Synergy 4 instrument, by Agilent Technologies (1 mentions) Halotag nanobret 618 ligand, by Promega (1 mentions) Pegfp n1 mammalian expression vector, by Takara Bio (1 mentions) Pfn31k nluc cmv neo flexi vector, by Promega (1 mentions) Nanobret nano glo detection system, by Promega (1 mentions) Envision plate reader, by PerkinElmer (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions) Celltiter glo 2.0 assay kit, by Promega (1 mentions)

Close spelling variants of this product

PFN21A HaloTag CMV Flexi HaloTag CMV Flexi Vector HaloTag-pFN21A PFN21A vectors Flexi vector PFN21A

4 protocols using pfn21a halotag cmv flexi vector

1

Overexpression of SYT13 in MKN74 cells

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SYT13 cDNAs were ligated to the pFN21A HaloTag CMV Flexi Vector (Promega, Madison, WI, USA). The SYT13 vector (0.2 mg) was used to transfect MKN74 cells (1 × 105) using the NEON Transfection System (Thermo Fisher Scientific, Waltham, MA, USA).
Kanda M., Kasahara Y., Shimizu D., Miwa T., Umeda S., Sawaki K., Nakamura S., Kodera Y, & Obika S. (2020). Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer. Molecular Therapy. Nucleic Acids, 22, 791-802.
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2

PALLD and ANKRD1/FHOD1 Interaction Study

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HEK293 cells cotransfected with pFN21A HaloTag CMV Flexi vector (Promega) expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 or FHOD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected 6 hr later using a Synergy 4 instrument (BioTek). Results were analyzed using GraphPad Prism 9.0 software.
Mastrototaro G., Carullo P., Zhang J., Scellini B., Piroddi N., Nemska S., Filomena M.C., Serio S., Otey C.A., Tesi C., Emrich F., Linke W.A., Poggesi C., Boncompagni S, & Bang M.L. (2023). Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities. eLife, 12, e78629.
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3

Heterologous Expression of TRPV1, TRPV4, and CD86

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The human TRPV1 (a gift kindly supplied by Asuka Inoue, Tohoku University) and human TRPV4 coding sequences (cloned from the MegaMan Human Transcriptome Library) were amplified with PCR and inserted into the pFN21A HaloTag® CMV Flexi® Vector (Promega, Madison, WI, USA) to fuse the HaloTag to the N-terminus of the expressed protein. The CD86 (M1-R277) coding sequence was amplified with PCR and inserted into the pEGFP-N1 mammalian expression vector (Clontech, CA, USA), in which the coding sequence of enhanced green fluorescent protein (EGFP) was substituted with that of HaloTag7.
Kuwashima Y., Yanagawa M., Abe M., Hiroshima M., Ueda M., Arita M, & Sako Y. (2021). Comparative Analysis of Single-Molecule Dynamics of TRPV1 and TRPV4 Channels in Living Cells. International Journal of Molecular Sciences, 22(16), 8473.
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4

Nanoscale Bioluminescence Resonance Energy Transfer Assay

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Wild type or mutant human KMO was cloned into a pFN21A HaloTag CMV Flexi Vector (Promega) and a pFN31K NLuc CMV-neo Flexi Vector (Promega). Cloned vectors were transfected into HEK293T cells using lipofectamine LTX (Invitrogen) and assayed according to the NanoBRET Nano-Glo Detection System (Promega) assay protocol. Briefly, transfected cells were divided into two pools with or without the HaloTag NanoBRET 618 Ligand and incubated overnight. NanoBRET Nano-Glo substrate was added and immediately detected using an Envision plate reader (PerkinElmer) at donor emission (460 nm) and acceptor emission (620 nm). Cell viability in the plates was determined by measuring luminescence using a CellTiter-Glo 2.0 Assay kit (Promega).
Mimasu S., Yamagishi H., Kubo S., Kiyohara M., Matsuda T., Yahata T., Thomson H.A., Hupp C.D., Liu J., Okuda T, & Kakefuda K. (2021). Full-length in meso structure and mechanism of rat kynurenine 3-monooxygenase inhibition. Communications Biology, 4, 159.
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