Tgfβ stimulated

Manufactured by Thermo Fisher Scientific

The TGFβ stimulated is a lab equipment product that serves a core function in research applications. It is designed to facilitate the study of transforming growth factor beta (TGFβ) signaling pathways. The product provides a controlled environment for researchers to investigate the effects of TGFβ stimulation on various cellular processes and responses.

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Lab products found in correlation

Sagm small airway epithelial cell growth medium, by Lonza (2 mentions) Bms 345541, by Merck Group (1 mentions)

2 protocols using tgfβ stimulated

Epithelial-Mesenchymal Transition in Immortalized hSAECs

Cited 2 times

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An immortalized primary human small airway epithelial cell (hSAEC) line was previously described.^{12 (link),14 (link)–18 (link)} The immortalized hSAECs were grown in SAGM small airway epithelial cell growth medium (Lonza, Walkersville, MD) in a humidified atmosphere of 5% CO₂. For induction of EMT, hSAECs were TGFβ stimulated for 14 d (10 ng/mL, PeproTech, Rocky Hill, NJ).^{14 (link)}

Zhang J., Jamaluddin M., Zhang Y., Widen S.G., Sun H., Brasier A.R, & Zhao Y. (2019). Type II Epithelial-Mesenchymal Transition Upregulates Protein N‑Glycosylation To Maintain Proteostasis and Extracellular Matrix Production. Journal of proteome research, 18(9), 3447-3460.

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Immortalization and EMT Induction of Human Small Airway Epithelial Cells

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An immortalized human small airway epithelial cell (hSAEC) line was established by infecting primary hSAECs with human telomerase (hTERT) and cyclin dependent kinase (CDK)-4 retrovirus constructs [21 (link)]. The immortalized hSAECs were grown in SAGM small airway epithelial cell growth medium (Lonza, Walkersville, MD) in a humidified atmosphere of 5 % CO₂. For induction of EMT, hSAECs were TGFβ stimulated for 15 days (10 ng/ml, PeproTech, Rocky Hill, NJ). The small molecule inhibitor of IKK, BMS345541 was purchased from Sigma Aldrich and used at 10 μM [22 (link)].

Tian B., Li X., Kalita M., Widen S.G., Yang J., Bhavnani S.K., Dang B., Kudlicki A., Sinha M., Kong F., Wood T.G., Luxon B.A, & Brasier A.R. (2015). Analysis of the TGFβ-induced program in primary airway epithelial cells shows essential role of NF-κB/RelA signaling network in type II epithelial mesenchymal transition. BMC Genomics, 16(1), 529.

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