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Anti rpgrip1l

Manufactured by Proteintech
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Anti-RPGRIP1L is a primary antibody product used for the detection and quantification of RPGRIP1L protein in various sample types. The antibody is designed to specifically recognize and bind to the RPGRIP1L protein, allowing for its identification and measurement in research applications.

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Lab products found in correlation

Hoechst, by Thermo Fisher Scientific (2 mentions) Anti ha hrp, by Roche (2 mentions) Anti ift20, by Proteintech (2 mentions) Mouse anti γ tubulin clone gtu 88, by Merck Group (2 mentions) Actub clone 6 11b 1, by Merck Group (2 mentions) Anti rhodopsin clone 4d2, by Merck Group (2 mentions) Anti β tubulin clone 2 28 33, by Merck Group (2 mentions) Anti ehd3, by Abnova (2 mentions) Rabbit anti pericentrin, by Novus Biologicals (2 mentions) Anti ehd2, by Abcam (2 mentions) Anti ehd4, by Abcam (2 mentions) Anti cep290, by Fortis Life Sciences (2 mentions) Anti tmem67, by Proteintech (2 mentions) Goat anti cep164, by Santa Cruz Biotechnology (2 mentions) Anti β actin clone ac 15, by Merck Group (2 mentions) Anti gfp, by Abcam (2 mentions) Hoescht, by Thermo Fisher Scientific (1 mentions) Epr5071, by Abcam (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp, by Roche (1 mentions)

Close spelling variants of this product

Anti-TM

3 protocols using anti rpgrip1l

1

Immunofluorescence Staining Protocol

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The following antibodies and dilutions were used: acetylated tubulin mouse monoclonal 6-11B-1 (1:1,000 for IF and WB) (Sigma-Aldrich Cat# T7451); pericentrin rabbit polyclonal Poly19237 (1:500 for IF) (Covance Cat #PRB-432C, now BioLegend); Arl13b rabbit polyclonal (1:250–1:500 for IF) (Proteintech Cat# 17711-1-AP); N19-SSTR3 antibody (rabbit polyclonal) (Santa Cruz Cat #sc-11610, discontinued); IFT88 rabbit (1:500 for IF and WB) (GeneTex, Cat#79169); α-tubulin YL1/2 (1:1,000 for IF and WB), (ThermoFisher #MA1-080017); alpha-tubulin DM1a (1:1,000 for IF and WB) (ThermoFisher #62204); rabbit monoclonal anti-p60 EPR5071, (1:250 IF), (Abcam Cat# ab111881); rabbit polyclonal anti-KATNA1, (1:100–250 IF) (Proteintech Cat#17560-1-AP), anti-RPGRIP1L (Proteintech 55160-1-AP). Anti-rabbit GFP (1:250) (Life Technologies, #A11122); anti-mouse GFP (1:1,000) (Roche, #11063100). Secondary antibodies used were anti-mouse Rhodamine (1:1,000) (Jackson ImmunoResearch, #715-295-150); anti-rabbit FITC (1:1,000) (Jackson ImmunoResearch, #111-095-003); anti-rabbit Alexa647 (1:200) (Life Technologies, #A21245); anti-mouse Alexa647 (1:200) (Life Technologies, #A21236); Hoescht (1:1,000–2,000) (Molecular Probes, #H-3570).
Mirvis M., Siemers K.A., Nelson W.J, & Stearns T.P. (2019). Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding. PLoS Biology, 17(7), e3000381.
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2

Antibody Panel for Cytoskeletal and Ciliary Proteins

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Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
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3

Antibody Panel for Cytoskeletal and Ciliary Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
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