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24 protocols using triethanolamine tea

1

Quantitative PLGA Composition Analysis

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Poly(lactic-co-glycolic acid) with a GA/LA ratio of 50:50 (RG 503H) and 75:25 (RG 752H) was purchased from Evonik Nutrition & Care GmbH (Darmstadt, Germany). The reference standards for GA (99%) and LA (≥95%) were purchased from Sigma Aldrich (Darmstadt, Germany). Poly(D,L-lactic acid) (≥95%) was purchased from Polysciences, Inc. (Hirschberg an der Bergstraße, Germany). For the derivatization, 2,4′-dibromoacetophenone (≥98%) and triethanolamine (TEA) (≥98%) were purchased from Sigma Aldrich (Darmstadt, Germany). The solvents acetonitrile, acetone, tetrahydrofuran, ethyl acetate, dimethylformamide, and chloroform were all purchased from Sigma Aldrich (Darmstadt, Germany). For the hydrolysis and pH adjustment, potassium hydroxide (KOH) and aqueous hydrobromic acid (HBr) were purchased from Sigma Aldrich (Darmstadt, Germany). The pH indicator bromocresol green was purchased from Honeywell (Seelze, Germany).
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2

Formulation and Characterization of Allantoin-Cyclodextrin Gels

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The materials used in this study were purchased and used as received: allantoin (Elton Chemical SA, Avlonas, Greece), β-cyclodextrin (β-CD) (Fluka, Ph. Eur.), hydroxypropyl-β-cyclodextrin (HP-β-CD) (Fluka, Ph. Eur.), Carbopol 934 or poly(acrylic acid), (PAA) (ServaFeinBiochemica, Heidelberg, Germany), triethanolamine (TEA) (Sigma-Aldrich, Schnelldorf, Germany), glycerol (CREMER OLEO, Hamburg, Germany), distilled water (FRX), NHDF (Normal Human Dermal Fibroblast) cell line from PromoCell, complete cell medium containing Eagle’s Minimal Essential Medium alpha, aMEM, Dulbecco’s Modified Eagle Medium without phenol red (DMEM), a mixture of penicillin-streptomycin-amphotericin B mixture of concentration of 1% (10 K/10 K/25 μg in 100 mL), EDTA (Trypsin-Versene mixture from Lonza), Fetal Bovine Serum (FBS) achieved from Biochrom GmbH (Berlin, Germany) and Phosphate Buffered Saline (PBS) from Invitrogen.
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3

Synthesis and Characterization of R-carvedilol

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R-carvedilol was synthesized by Chem-Impex International, Inc. (Wood Dale, IL, USA). The purity was determined by the manufacturer using Chiral HPLC as 98.97%. After the compound was received, the accuracy and purity were confirmed by chiral HPLC using Phenomenex Lux® 5 µm Cellulose-4 LC Column 250 × 4.6 mm (Phenomenex, Torrance, CA, USA). Tween-80, sodium cholate, and polyethylene glycol 400 (PEG 400) were purchased from VWR (Radnor, PA, USA). L-α-phosphatidylcholine (Soy PC or SPC), L-α-phosphatidylcholine hydrogenated (Hydro Egg PC, HEPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Carbopol® 934 was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). Triethanolamine (TEA) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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4

Carvedilol Formulation Development

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Carvedilol was purchased from TCI America (Portland, OR). Tween-80, Polyethylene glycol 400 (PEG 400), methanol, chloroform and phosphate-buffered saline (PBS) tablets were purchased from VWR (Radnor, PA). L-α-phosphatidylcholine (Soy PC or SPC) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Isoproterenol was purchased from Tocris (Minneapolis, MN). Carbopol 934 was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). Triethanolamine (TEA) was purchased from Sigma-Aldrich.
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5

Synthesis of Bismuth Molybdate Nanoparticles

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Bismuth(III) nitrate pentahydrate
[Bi(NO3)3·5H2O], sodium molybdate
dehydrate (Na2MoO4·2H2O), sodium
hydroxide (NaOH), and triethanolamine (TEA) were obtained from Sigma-Aldrich.
Concentrated nitric acid (HNO3) was obtained from Junsei
Chemical. All chemicals were of analytical grade and were used without
any further purification.
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6

Antioxidant Topical Formulation Development

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Paraffin oil (Merck KGOA, Darmstadt, Germany), acetyl alcohol (St. Louis, MO, USA), span 80 (Sigma-Aldrich, Darmstadt, Germany), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, Darmstadt, Germany), propyl paraben (Sigma-Aldrich, Darmstadt, Germany), Tween 80 (Sigma-Aldrich, Darmstadt, Germany), propylene glycol (Sigma-Aldrich, Darmstadt, Germany), methyl paraben (Sigma-Aldrich, Darmstadt, Germany), ethyl alcohol (Ethanol) (Sigma-Aldrich, Darmstadt, Germany), carbopol-940 (Sigma-Aldrich, St. Louis, MO, USA), triethanol amine (TEA) (Sigma-Aldrich, Darmstadt, Germany) were used in this research work. Purified water was manufactured at the laboratory of cosmeceuticals, Department of Pharmacy, IUB, Pakistan. Rotary evaporator (Heidolph, Co., Ltd., Schwabach, Germany, Bioevopeak Inc., Seattle, WA, USA), UV spectrophotometer (Boschaplatz 3 Presseck, 95355 Germany), microplate reader Synergy HT (BioTek Instrument, Stevens Creek Blvd, Santa Clara, CA, USA), refrigerator (Dawlance, Karachi, Pakistan), hot incubator (Sanyo MIR-162, Moriguchi City, Japan), digital humidity meter (TES Electronic Corp., Taipei, Taiwan), pH meter (WTW pH-197i, Weilheim, Germany), optical microscope (Eclipse E200, Nikon, Melville, NY, USA), and conductivity meter (WTW COND-197i, Weilheim, Germany) were used.
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7

Photocatalytic Degradation of Methyl Orange

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Analytical grade ammonium metavanadate (NH4VO3) ≥ 99.0%, cetyl trimethyl ammonium bromide (CTAB) ≥ 98%, sodium hydroxide (NaOH) ≥ 98%, methyl orange (MO) 85%, benzoquinone (BQ) ≥ 98%, triethanolamine (TEA) ≥ 99%, and isopropyl alcohol (IPA) ≥ 99.7% were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents were analytical grade and used without further purification. All the required solutions were prepared with distilled water.
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8

Diclofenac Soy Phospholipid Gel Formulation

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Diclofenac diethylamine and saturated phospholipid (soy phosphatidylcholine) were generous gifts from Biochem Pharmaceutical Industries (Mumbai, India) and Lipoid GmbH (Ludwigshafen, Germany), respectively. Triethanolamine (TEA) and sorbitan monooleate were procured from Sigma Chemicals Co. (St Louis, MO, U.S.A.). Carbopol 980 was obtained ex-gratis from Lubrizol Co. (Wickliffe, OH, U.S.A.). Diclofenac gel (Voveran® Emulgel®, Novartis India Limited, Bangalore) was procured from local pharmacy store. All other chemicals used in the study were of analytical grade.
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9

Synthesis and Characterization of PEG-HDI Polyurethanes

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Hexamethylene diisocyanate (HDI), di-n-butyltin dilaurate (DBTDL), and tri(ethylene glycol) (TEG) were purchased from Tokyo Chemical Industry Co., Japan. Poly(ethylene glycol) (PEG) (Mn = 1,000 g mol−1) was purchased from Kanto Chemical Co., Inc., Japan. Triethylamine and all organic solvents were purchased from Wako Pure Chemical Industries, Ltd., Japan. Triethanolamine (TEA) was purchased from Sigma-Aldrich, USA. HDI was vacuum distilled and stored under N2 atmosphere at 5°C before use. 1,4-Dioxane was distilled and stored under Ar atmosphere before use. Other chemicals were used as received. Bis(2-hydroxyethyl) diselenide (DSe) [53 ] and 2,6-bis(hydroxymethyl)-1-methylpyridin-1-ium iodide (iPDM) [57 ] were prepared according to the literature procedures.
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10

Novel Lipid-Based Nanoparticle Formulation

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (MPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphate (DPPA), 1,2 distearoyl-sn-glycero-3-phosphoethanolamine-N-Methoxypolyethyleneglycol-2000 (DSPE-PEG2k), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-Methoxypolyethyleneglycol-5000 (DPPE-PEG5k) were obtained from Avanti Polar Lipids Inc. (Alabaster, AL). Doxorubicin hydrochloride (USP grade), fatty acid- and globulin-free bovine serum albumin, copper (II) gluconate, ammonium sulfate, and triethanolamine (TEA) were purchased from Sigma (St. Louis, MO). Prohance was purchased from Merry X-Ray (Sacramento, CA). IRDye 800CW NHS ester was obtained from LI-COR Biosciences (Lincoln, NE). The neu deletion (NDL) metastatic mammary carcinoma cell line was obtained from the Alexander Borowsky Laboratory (UC Davis) [39 (link), 40 (link)]. TCC-SUP tumorigenic human bladder cancer cells were obtained from the American Type Culture Collection (HTB-5, Manassas, VA).
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