Synaptophysin syp

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Synaptophysin (SYP) is a protein used as a marker for synaptic vesicles in neurons. It is commonly used in immunohistochemistry and western blotting applications to identify and quantify synaptic density in tissue samples.

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Synaptophysin (19 citations)

2 protocols using synaptophysin syp

Comprehensive Molecular Profiling of Neurodegeneration

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The following primary antibodies were used: TH and NeuN (Merck-Millipore, MA, USA); Bcl-2, Bax, Cytochrome c, Caspase-3, PARP-1, phospho-JNK, JNK, Nurr1, DAT, GFAP, Iba-1, PSD-95, SNAP-25, Synaptophysin (SYP), phospho-mTOR (296. Ser2481) and β-actin (Santa Cruz, CA, USA); AMPKα, phospho-AMPKα, phospho-p44/42 MAPK (ERK1/2, Thr202/Tyr204), p44/42 MAPK (ERK1/2), phospho-p38 MAP kinase (Thr180/Tyr182), p38 MAPK, α-synuclein, phospho-CREB (Ser133), CREB, and mTOR (Cell Signaling, MA, USA); VMAT2 and phospho-α-synuclein (Ser129) (Abcam, Cambridge, UK); and phospho-α-synuclein (Ser129, BioLegend, CA, USA). Detailed antibody information is provided in Additional file 1: Table S1.

Park J.S., Choe K., Lee H.J., Park T.J, & Kim M.O. (2023). Neuroprotective effects of osmotin in Parkinson’s disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways. Journal of Biomedical Science, 30, 66.

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Immunohistochemical Evaluation of Cerebellar Synaptic Markers

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Cryocut (HS 525, Microm GmbH, Germany) sagittal sections (30 μm) of the cerebellum were processed for standardized free floating imunohistochemical technique [24] . Specific monoclonal antibodies: Synaptophysin (Syp: SantaCruz Biotechnology. sc9116) and Post Synaptic Density marker (PSD 95: Pierce Biotechnology, Inc. MA1045) in dilutions of 1:200 were used for primary incubation whereas Ultravision Plus Detection system kit (Thermo Scientific TP-060-HLX) was used as secondary antibody. 3′,3’ diaminobenzidine (DAB) was used as chromogen for visualizing immunoreactivity. Negative and positive controls were processed simultaneously by carrying out incubation with specific normal serum instead of primary antibody for the former and staining of hippocampus for the later.

Kaushal P., Kumar P, & Dhar P. (2019). Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum. Journal of Ayurveda and Integrative Medicine, 11(4), 455-463.

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