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3 protocols using tetrahydrofuran

1

Molecular Weight Determination of Membrane Samples

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The average molecular weight of the membrane samples was determined using a GPC system (Malvern 270 dual detector, UK) equipped with a refractive index detector and a light scattering detector (λ = 670 nm, consisting of two scattering angles: 7° and 90°) along with T6000M, a general mixed organic column (300 × 8 mm) (Viscotek, Malvern, UK). The detector and column were kept at a temperature of 22 °C. The mobile phase consisted of tetrahydrofuran (SD Fine Chemicals Ltd., India) at a flow rate of 0.5 mL min−1. The samples were filtered through a PTFE syringe filter with a 0.22 μm pore size and a 25 mm diameter (Axiva Sichem Biotech, India). The sample volume injected was 100 μL. Both detectors were calibrated with polystyrene standards having a narrow molecular-weight distribution. Data were analyzed using Viscotek OmniSEC 5.12 software.
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2

Synthesis and Evaluation of Polymeric Materials

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Acrylic acid, toluene, hydroquinone, tetrahydrofuran, and xylene were purchased from SD Fine Chemicals. p-toluene sulfonic acid and maleic anhydride were purchased from SRL. Polyethylene glycol (Mn=2000) was purchased from TCI chemicals; methyl iodide was purchased from Sigma Aldrich. Ethylene diamine, p-phenylene diamine, glutaraldehyde, and 1-docosanol were purchased from Merck and used as received. LB Agar media and Luria Bertani (LB) Broth purchased from Himedia, E. coli DH5α, B. Subtilis, B. Amyloliquefaciens, and C. Freundii strains were obtained from Microbial type culture collection at Chandigarh (MTCC).
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3

Organic Synthesis and Characterization

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Ferrocene (FC, purity 98%) was procured from Sigma Aldrich, India. Commercial activated carbon (AC, purity 98%) was also purchased from Sigma Aldrich, India. Acetonitrile (99%), methanol (99%), tetrahydrofuran (99%), n-hexane (99%), ethyl acetate (99%), pet ether (99%), n-pentane (99%) and chloroform solvents (purity 99%) were supplied by Sd Fine Chem. Limited. All substituted o-phenylenediamines and different substituted aliphatic and aromatic ketones were obtained from Sigma Aldrich, India (purity 99%).
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