Tmsp d4

Quercetin (≥95 %), tannic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), (+) - 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid - Trolox (≥97 %), type I agarose and anhydrous potassium persulfate (K₂S₂O₈), dimethyl sulfoxide (DMSO) were acquired from Sigma-Aldrich (Aldrich Brasil Ltda., Brazil). l-ascorbic acid (≥99.0 %), ethanol PA (99.5 %), and anhydrous sodium carbonate Na₂CO₃ (≥99.5) were obtained from Synth (LABSYNTH, Brazil), while ultrapure monohydrate gallic acid and aluminum chloride (III) (≥99.5 %) from Vetec (VETEC-Sigma-Aldrich, Brazil). The Folin-Ciocalteu reagent and bovine serum albumin–BSA were acquired from Merck (Merck, USA). Glacial acetic acid PA and sodium hydroxide were obtained from Dinâmica (Dinâmica, Brazil. Nutrient broth, malt extract, yeast extract, potato extract, and agar were purchased from Acumedia (Acumendia, USA). DMSO‑d₆ and TMSP-d4 (2,2,3,3-d4-3- sodium trimethylsilylpropionate) were purchased from Cambridge Isotope Laboratories, Inc. (USA). The reagents used in antimicrobial susceptibility tests were ciclopirox olamine [(LOPROX®), 10 mg g⁻¹, Sanofi-Aventis, Brazil)], DMSO [(≥99.0), Sigma-Aldrich (Aldrich Brasil Ltda., Brazil], DMEM Mix F12, penicillin G, gentamicin, amphotericin B, glucose, l-glutamine, and fetal from Gibco (Thermo Fisher Scientific, Brazil).

The M. thermoacetica-CdS was prepared as the previous method (6 (link)). Typically, 1 mM Cd(NO₃)₂ was added to the M. thermoacetica when OD₆₀₀ reached 0.42. After 3 d of growth, the opaque yellow suspension revealed the formation of M. thermoacetica-CdS. The MOF cytoprotected PBS was prepared in the same way as shown above. Before photosynthesis, 0.2 wt % cysteine was added to each tube. The sterilized O₂ was injected into each tube until 2% (vol/vol) to mimic the oxidative stress condition in the whole reaction. Each tube was stirred at 150 rpm and heated to a measured temperature of 55 °C by a stirring hot plate. The illumination source employed for simulated sunlight measurements was a collimated 75-W Xenon lamp (Newport, Corp.) with an AM 1.5 G filter. All light intensities were calibrated by a silicon photodiode (Hamamatsu S1787-04). Concentrations of photosynthetic products were measured by ¹H-qNMR with sodium 3-(trimethylsilyl)-2,2′,3,3′-tetradeuteropropionate (TMSP-d₄; Cambridge Isotope Laboratories, Inc.) as the internal standard in D₂O. Spectra were processed using the MestReNova software.