Gelatin (Bloom type-A; CAS No. 9000-70-8), Sodium tripolyphosphate (TPP; CAS No. 7758-29-4), Rhodamine-B (CAS No. 81-88-9), Cisplatin dichloride (CAS No. 15663-27-1), sodium borohydride (CAS No. 16940-66-2), dithiolated diethylenetriamine pentaacetic acid (DTDTPA), gold (III) chloride hydrate (CAS No. 27988-77-8) and glutaraldehyde (CAS No. 111-30-8) were purchased from Sigma-Aldrich (USA). Sterile ultraclean distilled water (Cat No. 10-977-015), sodium hydroxide (NaOH; CAS No. 1310-73-2), hydrochloric acid (HCl; CAS No. 7647-01-0), 200-proof ethanol (CAS No. 64-17-5), acetone (CAS No. 67-64-1), sodium chloride (NaCl; CAS No. 7647-14-5), sucrose (CAS No. 57-50-1), paraformaldehyde (PFA; Cat. No. 50-980-486), RPMI-media (Cat. No. A1049101) and fetal bovine serum (FBS; Cat. No. 26-140-079) were purchased from Thermo Fisher Scientific (USA). HS-PEG-OMe (Cat. No. 12750-40) was purchased from RAPP Polymere (Germany) and HS-PEG-COOH (Cat. No. CM-PEG–SH–2000) was purchased from Laysan Bio (USA). Doxorubicin (CAS No. 25316-40-9) was purchased from LC Laboratories (USA). Cy5 NHS ester (Cat. No. 23020) was purchased from Lumiprobe (USA). Iodixanol (Visipaque ®; Cat. No. 00407222317) was obtained from GE Healthcare (USA).
Cisplatin dichloride
Manufactured by Merck Group
Sourced in United States
Cisplatin dichloride is a platinum-based compound used as a laboratory reagent. It is a crystalline solid with the chemical formula PtCl2(NH3)2. This compound is commonly used in research applications, particularly in the fields of chemistry and biochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Sodium tripolyphosphate, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Doxorubicin, by LC Laboratories (1 mentions)
Cytarabine, by Merck Group (1 mentions)
Raltitrexed, by Merck Group (1 mentions)
Prestoblue, by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Compusyn software, by ComboSyn (1 mentions)
2 protocols using cisplatin dichloride
1
Synthesis and Characterization of Multifunctional Nanoparticles
2
Cytotoxicity Assessment of Anticancer Drugs
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The cytotoxicity of various drugs in cells was assessed by seeding 3000 cells into each well of a 96-well plate, followed by incubation overnight and treatment with various concentrations of the drugs for 72 h. Cisplatin dichloride, mitoxantrone dichloride, raltitrexed, cytarabine and paclitaxel were purchased from Sigma; 5-FU was purchased from Fluka; docetaxel, gemcitabine and oxaliplatin were obtained from the USP; gefitinib and irinotecan were purchased from Caymann; and doxorubicin dihydrochloride was obtained from Pfizer. Cell growth was determined by the PrestoBlue (Invitrogen, Carlsbad, CA, USA) assay using a microplate spectrometer and fluorescence microplate spectrophotometer. By aid of CompuSyn software (version 1.0.1; CompuSyn, Inc., Paramus, NJ, USA), the IC50 value of each drug was calculated based on the median-effect principle and plotted using the dose–effect relationship at nine concentrations [69 (link)]. The same protocol and software were adopted to determine the combination index (CI) of the combination treatment.
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