Decamethonium bromide

Manufactured by Merck Group

Sourced in United Kingdom, United States

Decamethonium bromide is a chemical compound used in laboratory settings. It functions as a neuromuscular blocking agent, which means it temporarily prevents the transmission of signals between nerves and muscles. This property makes it useful for various research applications, such as the study of muscle function and nerve-muscle interactions.

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Decamethonium bromide (DMBr) (2 citations)

4 protocols using decamethonium bromide

Autonomic Nerve Stimulation in Rabbit Hearts

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The cervical vagus nerves were stimulated using a pair of custom-made bipolar silver electrodes (VNS) (Advent Research Materials, UK: 0.5 mm diameter). Bilateral sympathetic nerve stimulation (SNS) was achieved by insertion of a quadripolar catheter into the spinal canal at the 12th thoracic vertebra to the level of stellate ganglia. Stimulation was delivered using 2-channel constant voltage square pulse stimulators, (S88, Grass Instruments), one for VNS and one for SNS at 2 ms pulse width. Intercostal-muscle twitching was prevented with a neuromuscular blocking agent (decamethonium bromide [Sigma, UK, 5 μM]).
Autonomic nerves were stimulated at varying strength (1-10 V) and heart rate (HR) response recorded. The stimulus strength that produced a heart rate equivalent to 80 % of the maximal response was used throughout. SNS was carried out at a frequency giving rise to an SNS-induced tachycardia of 230–250 bpm. VNS frequency was determined as the frequency resulting in a bradycardia of 60–70 bpm. The calibration protocol based on HR response was previously described, and allowed an unbiased comparison of ventricular electrophysiology between diseased and non-diseased Langendorff-perfused rabbit hearts in this study [8 (link)].

Chin S.H., Allen E., Brack K.E, & Ng G.A. (2023). Autonomic neuro-cardiac profile of electrical, structural and neuronal remodeling in myocardial infarction-induced heart failure. Journal of Molecular and Cellular Cardiology plus, 5, 100044.

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Enzymatic Assay for Protein Quantification

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Bovine serum albumin (BSA), ammonium sulfate, p-amino benzoic acid (PABA), p-nitro phenyl acetate (PNPA), trizma base (tris), iodo acetic acid, hexamethonium bromide, decamethonium bromide and protein estimation kit (Bradford Method) were obtained from Sigma. Phenyl methyl sulfonyl fluoride (USB, Switzerland) and DTT (SRL, India). All other chemicals and reagents were of analytical grade (Merck, India), unless or otherwise mentioned.

Gorain B., Chakraborty S., Pal M.M., Sarkar R., Samanta S.K., Karmakar S, & Sen T. (2014). Arylamine N-acetyl Transferase (NAT) in the blue secretion of Telescopium telescopium: xenobiotic metabolizing enzyme as a biomarker for detection of environmental pollution. SpringerPlus, 3, 666.

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Recombinant hAChE Expression and Purification

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Recombinant hAChE was expressed as a secreted, disulfide-linked dimer in drosophila S2 cells and purified by affinity chromatography as outlined previously [26 (link)]. Initial 0.5 mL fractions were maintained in 5 mM decamethonium bromide (Sigma-Aldrich Chemical Co., St. Louis, MO, USA) at 4 °C. Fractions were dialyzed against 20 mM sodium phosphate and 0.02% Triton X-100 (pH 7.0) at 4 °C, and 10- or 20- μL aliquots of the dialyzates were frozen at −20 °C until use. O,O-Diethyl O-(4-nitrophenyl) phosphate (paraoxon) and O,O-dimethyl O-(4-nitrophenyl) phosphate (paraoxon methyl) were commercial samples from Sigma-Aldrich Chemical Co (St. Louis, MO, USA).

, & Rosenberry T.L. (2020). Solvent Deuterium Oxide Isotope Effects on the Reactions of Organophosphorylated Acetylcholinesterase. Molecules, 25(19), 4412.

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Airway Responses in Allergic Mice

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To evaluate airway responses, mice were anesthetized by intraperitoneal (i.p.) administration of ketamine (100 mg/kg)/xylazine (5 mg/kg), and paralyzed with decamethonium bromide (25 mg/kg; Sigma-Aldrich), intubated and respirated at a rate of 120 breaths/min with a constant tidal volume (0.2 ml) 72 h after final allergen challenge. Methacholine (30 mg/ml for BALB/cJ and 200 mg/ml for C57BL/6J; Sigma-Aldrich) was injected into the inferior vena cava or delivered by nebulization and dynamic airway pressure (cm H₂0 × sec) was followed for 5 min. Serum and BAL fluid were collected, processed, and analyzed as described previously⁵⁷. In brief, blood was collected to measure total serum IgE concentrations. To collect BAL fluid, lungs were lavaged with Hank’s Balanced Salt Solution (HBSS, Invitrogen). Cells were recovered by centrifugation and total cells were counted (trypan blue exclusion). Slides were prepared and stained with Siemens Diff-Quik stain (Siemens Healthcare) for differential cell counts.

Smole U., Gour N., Phelan J., Hofer G., Köhler C., Kratzer B., Tauber P.A., Xiao X., Yao N., Dvorak J., Caraballo L., Puerta L., Rosskopf S., Chakir J., Malle E., Lane A.P., Pickl W.F., Lajoie S, & Wills-Karp M. (2020). Serum Amyloid A is a Soluble Pattern Recognition Receptor that Drives Type 2 Immunity. Nature immunology, 21(7), 756-765.

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