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Anti e2f3

Manufactured by ABclonal
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Anti-E2F3 is a laboratory product that specifically recognizes and binds to the E2F3 protein. E2F3 is a transcription factor that plays a crucial role in cell cycle regulation and proliferation. This product can be used for research purposes to study the expression, localization, and function of the E2F3 protein in various biological systems.

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Lab products found in correlation

Proteinase inhibitors cocktail, by Roche (2 mentions) Anti gsk3b, by ABclonal (2 mentions) Anti p107, by Proteintech (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Ripa lysis buffer, by Beyotime (2 mentions) Enhanced chemi luminescence (ecl), by PerkinElmer (2 mentions)

2 protocols using anti e2f3

1

Protein Extraction and Western Blot Analysis

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Protein extracts from tissues were prepared using RIPA lysis buffer (Beyotime). Tissues or cells were harvested and lysed in RIPA Buffer (50mM Tris (pH 7.4), 150mM NaCl, 1% Triton X-100, 1% sodium deoxycholate) containing proteinase inhibitors cocktail (Roche) on ice for 40 minutes. Lysates were centrifuged 40 minutes at 4°C at maximum speed (20000 rcf.) and resulting protein extracts separated by SDS–PAGE (12%). After transfer of proteins to PVDF membranes (Bio-rad), membranes were blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBS-T) for 1 hr at room temperature, followed by two washes with TBS-T. Membranes were incubated overnight at 4°C with primary antibodies followed by three 10-min washes in TBS-T, exposure to HRP-conjugated secondary antibody for 1 hr at room temperature, and 3 washes with TBS-T. Detection of HRP conjugated secondary antibody was performed with ECL (PerkinElmer). Antibodies used in supplemental experiment were anti-E2F3 (ABclonal Technology, A8811), anti-GSK3B (ABclonal Technology, A0479), Anti-P107 (Proteintech, 13354–1-AP).
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
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2

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracts from tissues were prepared using RIPA lysis buffer (Beyotime). Tissues or cells were harvested and lysed in RIPA Buffer (50mM Tris (pH 7.4), 150mM NaCl, 1% Triton X-100, 1% sodium deoxycholate) containing proteinase inhibitors cocktail (Roche) on ice for 40 minutes. Lysates were centrifuged 40 minutes at 4°C at maximum speed (20000 rcf.) and resulting protein extracts separated by SDS–PAGE (12%). After transfer of proteins to PVDF membranes (Bio-rad), membranes were blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBS-T) for 1 hr at room temperature, followed by two washes with TBS-T. Membranes were incubated overnight at 4°C with primary antibodies followed by three 10-min washes in TBS-T, exposure to HRP-conjugated secondary antibody for 1 hr at room temperature, and 3 washes with TBS-T. Detection of HRP conjugated secondary antibody was performed with ECL (PerkinElmer). Antibodies used in supplemental experiment were anti-E2F3 (ABclonal Technology, A8811), anti-GSK3B (ABclonal Technology, A0479), Anti-P107 (Proteintech, 13354–1-AP).
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
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