For prophylactic AA experiments, 2 mg of AA (Fisher Scientific: #ICN19462510) was diluted in 30 μL of corn oil (Sigma C8267) which was administered 4–6 hours prior to CS challenge via oral gavage. Control animals received 30 μL corn oil alone. All other experiments used saline controls. For exogenous Ang1 and Ang2 experiments, 500 ng of Ang1 or Ang2 (R&D Systems: #9936-AN and #7186-AN respectively) was administered via intraperitoneal injection 1 hour prior to CS challenge. For anti-Angiopoietin-2 antibody treatment 50 μg of antibody (Adipogen: #AG-27B-0016PF) was administered concurrently with CS challenge via intraperitoneal injection. For L-NAME experiments, 10 μg of L-NAME (Abcam: #120136) reconstituted in distilled water was injected via intraperitoneal injection concurrently with CS challenge. Finally, for L-Arginine experiments, 5 mg of L-Arginine (Cayman Chemical: #23703) reconstituted in distilled water was administered via intraperitoneal injection 4-6 hours prior to CS challenge. The schedules described above were maintained for all combinational interventions described.
L arginine
Manufactured by Cayman Chemical
L-arginine is an amino acid that serves as a building block for proteins. It is commonly used in biochemical and cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Ag 27b 0016pf, by Adipogen (1 mentions)
L name, by Abcam (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Adenosine, by Merck Group (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
L arginine, by Merck Group (1 mentions)
Diclofenac, by Cayman Chemical (1 mentions)
Tamsulosin hydrochloride, by Merck Group (1 mentions)
Butoxamine, by Merck Group (1 mentions)
Mirabegron, by Cayman Chemical (1 mentions)
Sildenafil, by Cayman Chemical (1 mentions)
Vardenafil, by Cayman Chemical (1 mentions)
Y 27632 dihydrochloride, by R&D Systems (1 mentions)
Rolipram, by Cayman Chemical (1 mentions)
Celecoxib, by Cayman Chemical (1 mentions)
Sterile cell culture grade dmso, by Merck Group (1 mentions)
L glucose, by Thermo Fisher Scientific (1 mentions)
3 protocols using l arginine
1
Prophylactic and Therapeutic Interventions for Allergic Airway Inflammation
2
Preparation of Compound Stock Solutions
3
Glucose-6-Phosphate Quantification in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HOS and U2OS cells were incubated with L-arginine (cat. no. A0013; Beijing Solarbio Science & Technology Co., Ltd.) and glucose-free F-12K (cat. no. 21127022, Gibco; Thermo Fisher Scientific, Inc.) medium for 30 min at 37°C and then incubated with 7 mM L-glucose (cat. no. 20829; Cayman Chemical Company), 1 mM L-arginine, or 1 mM D-arginine (cat. no. A769515; Toronto Research Chemicals) for another 30 min at 37°C. Cell lysates were then collected and glucose 6-phosphate content was determined using a Glucose-6-Phosphate Fluorometric Assay kit (cat. no. 700750-96 wells; Cayman Chemical Company). Finally, the concentration of glucose 6-phosphate in cells was determined according to the methods reported in the literature (27 (link)).
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