The following first antibodies were purchased: rabbit anti-HNRNPU (#A3917), rabbit anti-PUF60 (#A6709), rabbit anti-YBX1(#A7704), rabbit anti-DDX9 (#A4563), rabbit anti-NCL (#A5904), rabbit anti-ILF3 (#A8186), rabbit anti-DKC1(#A1862), rabbit anti-TOP2A (#A16440), rabbit anti-RPA70 (#A0990) and rabbit anti-STAU1 (#A4131) from Abclonal (Woburn, MA); mouse anti-Ku70 (#SC-17789) from Santa Cruz (Dallas, TX); rabbit anti-Ku80 (#2180S) and rabbit anti-His (#12698S) from Cell Signaling; mouse anti-Flag (#200-301-B13) from Rockland (Limerick, PA); mouse anti-β-actin (#A5441) from MilliporeSigma; anti-GST (#SC-138) from Santa Cruz (Dallas, TX); rabbit anti-Strep-tag II (#ab183907) from Abcam (Waltham, MA);
Rabbit anti his
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-His is a polyclonal antibody raised in rabbits against a recombinant protein containing a histidine (His) tag. It is designed for the detection of His-tagged proteins in a variety of applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti hnrnpu, by ABclonal (1 mentions)
Rabbit anti ncl, by ABclonal (1 mentions)
Rabbit anti ku80, by Cell Signaling Technology (1 mentions)
Mouse anti ku70, by Santa Cruz Biotechnology (1 mentions)
Mouse anti flag, by Rockland Immunochemicals (1 mentions)
Rabbit anti strep tag 2, by Abcam (1 mentions)
Anti gst, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
D4008, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg hrp conjugate, by Cell Signaling Technology (1 mentions)
Goat anti mouse igg hrp conjugate, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti v5, by Cell Signaling Technology (1 mentions)
Mouse anti flag, by Cell Signaling Technology (1 mentions)
Axio imager m2, by Zeiss (1 mentions)
3 protocols using rabbit anti his
1
Antibody Reagents for Protein Analysis
2
Trafficking Pathway Analysis of Vip3Aa-RFP
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Primary antibodies: Mouse anti-Flag (Cell Signaling 8146), rabbit anti-V5 (Cell Signaling 13202), rabbit anti-His (Cell Signaling 12698), anti-Sf-SR-C-N polyclonal antibodies were generated by immunizing rabbits with purified GST-SR-C-N. Secondary antibodies: goat anti-mouse IgG-HRP conjugate (Santa Cruz sc-2005), goat anti-rabbit IgG-HRP conjugate (Cell Signaling 7074), rabbit anti-GST (Polyclonal, Bioss bs-2735R). The primary antibodies and secondary antibodies were used at 1: 1000 for western blotting. For immunostaining assays, Anti-V5-Dylight 488 conjugate (Invitrogen MA5-15253-D488, 1:200) and Alexa Fluor 488 goat anti-rabbit IgG (Cell Signaling 4412, 1:200) were used.
Inhibitors: Dynasore (dynamin inhibitor, TargetMol T1848, 7.5μM), chlorpromazine (clathrin-mediated endocytosis inhibitor, Millipore 215921, 40μM), monodansylcadaverine (clathrin-mediated endocytosis inhibitor, Sigma D4008, 150μM), nystatin (sequesters cholesterol, Millipore 475914, 20μM), cholesterol-oxidase (oxidize cholesterol, Millipore 228230, 4unit/ml), amiloride (macropinocytosis inhibitor, Millipore 129876, 150μM), Cytochalasin D (macropinocytosis inhibitor, Millipore 250225, 500nM), LY294002 (broad PI(3)K inhibitor, Cell Signaling 9901s, 50μM), and wortmannin (broad PI(3)K inhibitor, Cell Signaling 9951s, 2μM). Cells were treated with the inhibitors for 1 h at 27°C before Vip3Aa-RFP was added.
Inhibitors: Dynasore (dynamin inhibitor, TargetMol T1848, 7.5μM), chlorpromazine (clathrin-mediated endocytosis inhibitor, Millipore 215921, 40μM), monodansylcadaverine (clathrin-mediated endocytosis inhibitor, Sigma D4008, 150μM), nystatin (sequesters cholesterol, Millipore 475914, 20μM), cholesterol-oxidase (oxidize cholesterol, Millipore 228230, 4unit/ml), amiloride (macropinocytosis inhibitor, Millipore 129876, 150μM), Cytochalasin D (macropinocytosis inhibitor, Millipore 250225, 500nM), LY294002 (broad PI(3)K inhibitor, Cell Signaling 9901s, 50μM), and wortmannin (broad PI(3)K inhibitor, Cell Signaling 9951s, 2μM). Cells were treated with the inhibitors for 1 h at 27°C before Vip3Aa-RFP was added.
3
Immunohistochemical Detection of HIS Proteins
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Liver, spleen, and pancreas samples were collected and fixed in 10% neutral buffered formalin for HIS protein detection by the immunohistochemistry test. Paraffin-embedded sections of 10 μm thickness were prepared from these fixed tissue samples and then were deparaffinised and sequentially rehydrated. The immunohistochemistry assay was performed according to the method described by Jia et al. [40 (link)]. Primary (rabbit anti-HIS; Cell Signaling Technology, Danvers, MA, USA) and secondary (goat anti-rabbit immunoglobulin G Alexa 488 antibody; Thermo Fisher Scientific, Waltham, MA, USA) antibodies were diluted in blocking buffer with concentrations recommended by the manufacturers. Sections stained with only secondary antibody were used as negative controls. Images were captured using a Zeiss Axio Imager M2.
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