F. nucleatum (ATCC 25586), P. anaerobius (ATCC 27337), and P. micra (ATCC 33270) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). All bacteria were cultured at 37°C in Anaerobe basal broth (CM0957, OXOID, Thermo Fisher Scientific, West Palm Beach, FL, USA) in an anaerobic jar (Hardy Diagnostics, Santa Maria, CA, USA). Anaerobic conditions were maintained using AnaeroGen packets (Thermo Fisher Scientific).
P micra
Manufactured by American Type Culture Collection
Sourced in United States
P. micra is a laboratory product offered by the American Type Culture Collection (ATCC). It is a microbiological specimen that serves as a reference material for research and testing purposes. The core function of P. micra is to provide a standardized and authenticated sample for scientific investigations, without making any interpretations or extrapolations about its intended use.
Automatically generated - may contain errors
Lab products found in correlation
F nucleatum, by American Type Culture Collection (2 mentions)
Anaerobic jar, by Hardy Diagnostics (1 mentions)
Anaerobe basal broth, by Thermo Fisher Scientific (1 mentions)
Brain heart infusion broth, by HiMedia (1 mentions)
Menadione, by Merck Group (1 mentions)
P aeruginosa, by American Type Culture Collection (1 mentions)
P gingivalis, by American Type Culture Collection (1 mentions)
S mutans, by American Type Culture Collection (1 mentions)
C albicans, by American Type Culture Collection (1 mentions)
Hemin, by Merck Group (1 mentions)
Yeast nitrogen base broth, by Merck Group (1 mentions)
S aureus, by American Type Culture Collection (1 mentions)
E faecalis, by American Type Culture Collection (1 mentions)
Brucella broth, by Acumedia (1 mentions)
2 protocols using p micra
1
Culturing Anaerobic Oral Bacteria
2
Standardized Microbial Culture Conditions
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Reference strains from American Type Culture Collection (ATCC) were used such as C. albicans (ATCC 18804), E. faecalis (ATCC 4083), P. aeruginosa (ATCC 15442), S. aureus (ATCC 6538), S. mutans (ATCC 35688), F. nucleatum (ATCC 25586), P. endodontalis (ATCC 35406), P. gingivalis (ATCC 33277), and P. micra (ATCC 23195). The microorganisms were frozen at -80 °C, and activated in a specific culture medium according to the metabolic needs, being Yeast Nitrogen Base broth (YNB -Sigma-Aldrich, St. Louis, USA), for C. albicans, and Brain Heart Infusion broth (BHI -Himedia), for aerobic bacteria. They were incubated at 37 °C for 24 h, with 5% CO2 for S. mutans. For anaerobic bacteria, Brucella broth (Acumedia, Michigan, USA) supplemented with 5% sterile defibrinated sheep blood (Newprov, Pinhais-PR, Brazil), 1% hemin (Sigma-Aldrich), and 1% menadione (Sigma-Aldrich) was used. These bacteria were incubated in anaerobiosis with nitrogen 80.01%, carbon dioxide 10.02%, and hydrogen 9.97%; (Whitley DG250 Workstation, West Yorkshire, UK) at 37 ºC for 48 h.
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