Rabbit anti ciap2

Manufactured by Cell Signaling Technology

Sourced in Germany

Rabbit anti-cIAP2 is a polyclonal antibody raised in rabbits against the cIAP2 (cellular inhibitor of apoptosis protein 2) protein. cIAP2 is a member of the inhibitor of apoptosis (IAP) family of proteins, which play a role in regulating apoptosis (programmed cell death) by inhibiting caspase activity. This antibody can be used to detect and study the expression and localization of cIAP2 in various cell and tissue samples.

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3 protocols using rabbit anti ciap2

Comprehensive Western Blot Analysis of Cell Death Regulators

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Western blot analysis was performed as described previously [29] (link), using the following antibodies: rabbit anti-MLKL, rabbit anti-RIPK3, rabbit anti-phospho MLKL, rabbit anti-phospho RIKP1, rabbit anti-phospho RIPK3, rabbit anti-TNFR1, rabbit anti-cIAP2 (all from Cell Signaling), mouse anti-RIPK1 (BD Bioscience), mouse anti-GAPDH (BioTrend), rabbit anti-TRAIL-R1 (Abcam), rabbit anti-TRAIL-R2 (Millipore), rabbit anti-FasR, mouse anti-cIAP1 (all from Santa Cruz), mouse anti-Caspase-8 (Enzo). For detection, secondary antibodies goat anti-mouse IgG and goat anti-rabbit IgG (Abcam) conjugated to horseradish peroxidase and enhanced chemiluminescence were used (Amersham Bioscience).

Koch A., Jeiler B., Roedig J., van Wijk S.J., Dolgikh N, & Fulda S. (2021). Smac mimetics and TRAIL cooperate to induce MLKL-dependent necroptosis in Burkitt's lymphoma cell lines. Neoplasia (New York, N.Y.), 23(5), 539-550.

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Immunoblotting of Cell Signaling Proteins

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Immunoblotting was performed using the following antibodies: mouse anti-cIAP1 (Santa Cruz, Heidelberg, Germany; 271418), rabbit anti-cIAP2 (Cell Signaling Technologies, Leiden, The Netherlands; 3130), rabbit anti-survivin (R&D; AF886), mouse anti-XIAP (BD, Franklin Lakes, NJ, USA; 610716), rabbit anti-IκBα (Cell Signaling Technologies; 9242), mouse anti-p-IκBα (Cell Signaling Technologies; 6249L), mouse anit-p65 (Santa Cruz; sc-8008), rabbit anti-p-p65 (Cell Signaling Technologies; 3033S), rabbit anti-NIK (Cell Signaling Technologies; 4994), mouse anti-p100 (Merck-Millipore, Darmstadt, Germany; 05-361), mouse anti-GAPDH (BioTrend, Köln, Germany; NB-29-00852), mouse anti-vinculin (Sigma/Merck; V9131), mouse anti-caspase-8 (Enzo; ADI-AAM-118-E), rabbit anti-caspase-9 (Cell Signaling Technologies; 9502S), rabbit anti-DR5 (Merck-Millipore; AB16942) and mouse anti-PARP-1 (Cell Signaling Technologies; 9546S). The following secondary horseradish peroxidase-conjugated antibodies were used: goat anti-mouse (Abcam, Berlin, Germany; ab6789) and goat anti-rabbit (Abcam; ab6721). The quantification of Western blot and the area determination of the region of interest were performed using ImageJ/FIJI [28 (link)]. Subsequent ratio calculations were performed using Microsoft Excel.

Särchen V., Reindl L.M., Wiedemann S., Shanmugalingam S., Bukur T., Becker J., Suchan M., Ullrich E, & Vogler M. (2023). Characterization of BV6-Induced Sensitization to the NK Cell Killing of Pediatric Rhabdomyosarcoma Spheroids. Cells, 12(6), 906.

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Antibodies for Protein Expression Analysis

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Rabbit anti-RIPK2 (Cell Signaling, 4142), mouse anti-tubulin (Sigma, T8328), mouse anti-actin (Sigma, A2228), mouse anti-β-actin (Abcam, ab6276), rabbit anti-β-actin (Abcam, ab8227), mouse anti-vinculin (Abcam, ab129002), rabbit anti-cIAP1 (Cell Signaling, 7065), rabbit anti-cIAP2 (Cell Signaling, 3130), mouse anti-XIAP (BD Biosciences, 610717), donkey anti-rabbit IRdye 800CW (Licor, 926-32213), donkey anti-mouse IRdye 680RD (Licor, 926-68072), 20x anti-rabbit HRP conjugate antibody (Protein Simple, 043-426), anti-mouse HRP conjugate antibody (Protein Simple, 042-205), anti-rabbit HRP conjugate antibody (ProteinSimple, 042-206).

Mares A., Miah A.H., Smith I.E., Rackham M., Thawani A.R., Cryan J., Haile P.A., Votta B.J., Beal A.M., Capriotti C., Reilly M.A., Fisher D.T., Zinn N., Bantscheff M., MacDonald T.T., Vossenkamper A., Dace P., Churcher I., Benowitz A.B., Watt G., Denyer J., Scott-Stevens P, & Harling J.D. (2020). Extended pharmacodynamic responses observed upon PROTAC-mediated degradation of RIPK2. Communications Biology, 3, 140.

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