Urinary exosomal vesicle suspension in 1X PBS was applied to 300 mesh carbon coated copper grids (Ted Pella Inc., CA, USA). The adsorbed exosomes were negatively stained with 1% aqueous uranyl acetate. The samples were examined with a JEM 2100F electron microscope (JEOL, Peabody, MA, USA) operating at 200 kV.
300 mesh carbon coated copper grid
Manufactured by Ted Pella
Sourced in United States
The 300-mesh carbon-coated copper grid is a sample preparation tool used in electron microscopy. It provides a stable, conductive support for specimens to be viewed under an electron beam.
Automatically generated - may contain errors
Lab products found in correlation
100cx 2 tem, by JEOL (2 mentions)
Megaview 3 ccd camera, by Olympus (2 mentions)
Jem 2100f electron microscope, by JEOL (1 mentions)
Quanta 400 esem feg, by Thermo Fisher Scientific (1 mentions)
Sem stub, by Ted Pella (1 mentions)
Edx detector, by Bruker (1 mentions)
Nano series, by Malvern Panalytical (1 mentions)
Zetasizer nano series, by Malvern Panalytical (1 mentions)
Du800, by Beckman Coulter (1 mentions)
Jem 1400, by JEOL (1 mentions)
Tecnai t12, by Thermo Fisher Scientific (1 mentions)
Jem 2010, by JEOL (1 mentions)
13 protocols using 300 mesh carbon coated copper grid
1
Urinary Exosome Visualization via TEM
2
Imaging Hybrid Nanogel Particles by SEM and TEM
3
Polyplexes Characterization by Electron Microscopy
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Three
μL of polyplex solution (formulated with pDNA and polymers pMAT-b-AEMA-1, -2, or -3) prepared at N/P = 10 were applied to
a 300-mesh carbon coated copper grid (Ted Pella, Inc., Redding, CA)
and negatively stained with uranyl acetate. Images were saved as TIFF
files and polyplexes sized (excluding polyplexes on image edges) by
counting pixels using Microsoft Paint, and the sizes are plotted inFigure S9 .
μL of polyplex solution (formulated with pDNA and polymers pMAT-b-AEMA-1, -2, or -3) prepared at N/P = 10 were applied to
a 300-mesh carbon coated copper grid (Ted Pella, Inc., Redding, CA)
and negatively stained with uranyl acetate. Images were saved as TIFF
files and polyplexes sized (excluding polyplexes on image edges) by
counting pixels using Microsoft Paint, and the sizes are plotted in
4
TEM Characterization of Colloidal AgNPs
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The samples were examined in the high contrast mode on a Hitachi High-Tech HT7700 transmission electron microscope (Hitachi High-Technologies Corporation, Tokyo, Japan) at 120 kV accelerating voltage. The instrument is equipped with a Bruker EDX detector that allows elemental analysis and a SAED aperture that could be used to collect diffraction patterns directly during the sample inspection. A drop of each colloidal AgNPs solution was placed on a 300-mesh carbon-coated copper grid (Ted Pella) and vacuum-dried at room temperature for 24 h prior to examination.
5
Characterization of LPH Nanoparticles
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The size and surface charge of LPH cores and final particles were determined by using a Malvern ZS90 Zetasizer (Worcestershire, UK). Transmission electron microscope images of LPH were acquired through the use of JEOL 100CX II transmission electron microscope (Tokyo, Japan). Briefly, freshly prepared LPH nanoparticles (5 μl) were carefully dropped onto a 300-mesh carbon-coated copper grid (Ted Pella, Inc., Redding, CA) and allowed to stand at room temperature for 5 min. Grids were then stained with 1% uranyl acetate (5 μl) and allowed to incubate briefly (10 s) and quickly dry. All images were acquired at an accelerating voltage of 100 kV.
6
Transmission Electron Microscopy of LPH Nanoparticles
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Example 3
Transmission electron microscope (TEM) images of LPH were acquired through the use of JEOL 100CX II TEM (Tokyo, Japan). Briefly, freshly prepared LPH nanoparticles (5 μL) were carefully dropped onto a 300-mesh carbon-coated copper grid (Ted Pella, Inc., Redding, Calif.) and allowed to stand at room temperature for 5 min. Grids were then stained with 1% uranyl acetate (5 μL) and allowed to incubate briefly (10 seconds) and quickly dry. All images were acquired at an accelerating voltage of 100 kV.
7
Characterization of Polymeric Nanoparticles
8
Characterization of IEP-PEG Nanoparticles
9
Characterization of DAC and c-DAC Nanostructures
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The surface morphology of DAC and
c-DAC samples was characterized by a scanning electron microscopy
instrument (SEM, Zeiss, LEO 1550 SFEG) equipped with energy-dispersive
X-ray analysis (EDX) for elemental mapping (EHT = 2.5 kV). TEM images
of these samples were acquired using a transmission electron microscopy
instrement (TEM, JEOL, JEM-1400) operated at 80 kV. The sample preparation
for TEM measurements was as follows: DAC and c-DAC suspensions were
diluted to 0.01 wt% and subsequently sonicated for 1 h. Then, 10 μL
of suspension was deposited onto a carbon-coated 300-mesh copper grid
(Ted Pella Inc.), followed by staining using a drop of 2.0 wt% uranyl
acetate for 10 s. The Brunauer–Emmett–Teller (BET) specific
surface areas of DAC and c-DAC were analyzed via the N2 adsorption–desorption isotherms using a NOVAtouch LX2 (Quantachrome)
instrument. The zeta potentials of DAC and c-DAC suspensions (0.1
wt%) were measured via a Zetaprobe analyzer (Colloidal Dynamics) to
investigate their surface charge.
c-DAC samples was characterized by a scanning electron microscopy
instrument (SEM, Zeiss, LEO 1550 SFEG) equipped with energy-dispersive
X-ray analysis (EDX) for elemental mapping (EHT = 2.5 kV). TEM images
of these samples were acquired using a transmission electron microscopy
instrement (TEM, JEOL, JEM-1400) operated at 80 kV. The sample preparation
for TEM measurements was as follows: DAC and c-DAC suspensions were
diluted to 0.01 wt% and subsequently sonicated for 1 h. Then, 10 μL
of suspension was deposited onto a carbon-coated 300-mesh copper grid
(Ted Pella Inc.), followed by staining using a drop of 2.0 wt% uranyl
acetate for 10 s. The Brunauer–Emmett–Teller (BET) specific
surface areas of DAC and c-DAC were analyzed via the N2 adsorption–desorption isotherms using a NOVAtouch LX2 (Quantachrome)
instrument. The zeta potentials of DAC and c-DAC suspensions (0.1
wt%) were measured via a Zetaprobe analyzer (Colloidal Dynamics) to
investigate their surface charge.
10
Negative Staining of Protein Aggregates
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When noted, samples
were centrifuged at 14,000g for 45 min to remove
fibrils for improved contrast of smaller aggregates. Supernatant or
whole solution samples (5 μL) were deposited to a previously
discharged carbon-coated 300 mesh copper grid (Ted Pella, Inc.) and
allowed to sit for 5 min. The excess liquid was washed with water
and removed with filter paper. Grids were then negatively stained
for 2 min with 2% uranyl acetate, and excess liquid was removed with
filter paper and again washed. The negatively stained samples were
dried in air and imaged on an FEI Tecnai T12 transmission electron
microscope operated at 120 kV. Images of the total solution (without
centrifugation) were also obtained by the same method.
were centrifuged at 14,000g for 45 min to remove
fibrils for improved contrast of smaller aggregates. Supernatant or
whole solution samples (5 μL) were deposited to a previously
discharged carbon-coated 300 mesh copper grid (Ted Pella, Inc.) and
allowed to sit for 5 min. The excess liquid was washed with water
and removed with filter paper. Grids were then negatively stained
for 2 min with 2% uranyl acetate, and excess liquid was removed with
filter paper and again washed. The negatively stained samples were
dried in air and imaged on an FEI Tecnai T12 transmission electron
microscope operated at 120 kV. Images of the total solution (without
centrifugation) were also obtained by the same method.
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