Millipore purifier

For HPLC analysis, chromatographic-grade methanol was used (VWR, Vienna, Austria). A Millipore purifier (Millipore, Burlington, MA, USA) was used to obtain water for HPLC. Potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid, potassium chloride, quercetin, hispiduline, ascorbic acid, ibuprofen, ketoprofen, sodium chloride, hydrogen peroxide, trypsin, Tris-HCl buffer, sodium nitroprusside, sodium salicylate, ferrous sulfate, sulfanilamide, naphthylethylenediamine dihydrochloride and perchloric acid were purchased from Sigma-Aldrich, Taufkirchen, Germany. Human albumin 20%—BB, 200 g/L was ordered from BB-NCIPD Ltd., Sofia, Bulgaria.

Chromatographic-grade methanol (VWR, Vienna, Austria) was used for HPLC analysis. Water for HPLC was obtained using a Millipore purifier (Millipore, Burlington, MA, USA). Hexane, chloroform, butanol, ethyl acetate, ethanol, acetonitrile, ibuprofen, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium chloride, potassium chloride, sodium carbonate, dibenzylidene acetone, cinnamic acid, sodium salicylate, ferrous sulfate, ferrous dichloride, sodium nitrite, sulfanilamide, phosphoric acid, naphthylethylenediamine dihydrochloride, ferrozine, sodium nitroprusside, chlorogenic acid, phloroglucinol, hydrogen peroxide, ascorbic acid, quercetin, trypsin, Tris-HCl buffer, Folin–Ciocalteu’s reagent, aluminum trichloride, EDTA, sodium hydroxide, formic acid (FA), and perchloric acid were supplied by Sigma-Aldrich Chemie GmbH, Buchs, SG, Switzerland. Human albumin 20%—BB, 200 g/L was purchased from BB-NCIPD Ltd., Sofia, Bulgaria.

The samples of Z. armatum, harvested and dried in July 2020, were collected from Hongya county, Sichuan province, China. The dried Z. armatum fruits for this research were full-grained, with complete oil cells.
Ethanol (EtOH), petroleum ether (60–90 °C), and ethyl acetate were purchased from Chengdu Jere Technology Co., Ltd. (Chengdu, China). HPLC grade methyl alcohol and acetonitrile (ACN) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Standard hydroxyl-α-sanshool was purchased from Chengdu RefMedic Biotech Co., Ltd. (Chengdu, China, purity ≥ 98.00%). Silica gel (100–200 mesh) and thin-layer chromatography (TLC) plates (silica gel GF254, 100 × 200 mm) were obtained from Qingdao Haiyang Chemical Co., Ltd. (Qingdao, China). Ultrapure water was prepared using a Millipore purifier (Millipore, Burlington, MA, USA).