Glass capillaries

Manufactured by Zeiss

Sourced in Germany

Glass capillaries are narrow, cylindrical glass tubes with a small internal diameter. They are designed to transport and handle small volumes of liquids or gases through capillary action. The core function of glass capillaries is to provide a precise and controlled means of fluid handling in various scientific and industrial applications.

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Lab products found in correlation

Lightsheet z 1, by Zeiss (2 mentions) Lightsheet z 1 microscope, by Zeiss (2 mentions) Z 1 light sheet microscope, by Zeiss (2 mentions) Na 0.2 illumination optics, by Zeiss (2 mentions) W plan apochromat 20 1.0 objective, by Zeiss (2 mentions) Zen blue software, by Zeiss (1 mentions) Low melting point agarose, by Merck Group (1 mentions) Tricaine, by Merck Group (1 mentions) Tricaine, by Zeiss (1 mentions) Igg h l alexa fluor 488, by Abcam (1 mentions) Arivis software, by Zeiss (1 mentions) Lightsheet microscope v1, by Zeiss (1 mentions) Topvision low melting point agarose, by Thermo Fisher Scientific (1 mentions)

7 protocols using glass capillaries

Zebrafish Embryo Live Imaging via Lightsheet Microscopy

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Zebrafish embryos were staged to 14 hpf and mounted in 1% low melting
agarose (MidSci IB70051 St. Louis, Missouri) dissolved in E3 medium and 0.2
mg/ml tricaine using glass capillaries (Carl Zeiss Microscopy, 701904). Embryos
were incubated at 28.5°C during data collection. Images were captured
with a Zeiss Lightsheet Z.1 single-plane illumination microscope (Carl Zeiss
Microscopy) with tandem PCO.edge sCMOS cameras (PCO.Imaging, Kelheim, Germany)
and Zeiss Zen imaging software. A 20×/1.0 long working distance detection
objective was used alongside a pair of 10×/0.2 dry illumination
objectives, and the excitation sheet was narrowed to 2.0 μm. Volumes were
acquired every 5 min between 15 and 21 hpf, with 119 ms exposure per slice for
both green (488 nm, 7.5%) and red (561 nm, 7.0%) channels. Cell tracks were
manually reconstructed using Imaris.

Rocha M., Kushkowski E., Schnirman R., Booth C., Singh N., Beadell A, & Prince V.E. (2021). Zebrafish Cdx4 regulates neural crest cell specification and migratory behaviors in the posterior body. Developmental biology, 480, 25-38.

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Live Imaging of Floral Bud Development

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Live imaging was performed as previously described (Valuchova et al., 2020 (link)). Floral buds .3 to .7 mm long were selected from the main inflorescence. The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing 1/2 Murashige and Skoog (MS) medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light‐sheet microscope. After the holder is inserted in the microscope chamber, the remaining space is filled with liquid 1/2 MS medium (5% sucrose, pH 5.8). Images were taken every 5 min with a 10× objective (detection optics 10×/.5), single illumination (illumination optics 10×/.2), 561‐nm laser (15% intensity). The large raw data files were processed by ZEN Blue software (Zeiss).

Tanasa S., Shukla N., Cairo A., Ganji R.S., Mikulková P., Valuchova S., Raxwal V.K., Capitao C., Schnittger A., Zdráhal Z, & Riha K. (2023). A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis. Plant Direct, 7(3), e477.

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Zebrafish Embryo Anesthesia and Imaging

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Zebrafish embryos were first anesthetized in E3 media containing 0.02% Tricaine (Sigma), then embedded and centered within glass capillaries (1.0 mm inner diameter, Zeiss) filled with low-melting point agarose (LMP, 2% in E3 medium, 0.02% Tricaine). After setting, an agarose cylinder containing the anterior portion of the embryo was extruded from the capillary by inserting the opposite end into a microfuge tube containing 2% LMP agarose. The imaging chamber was filled with E3 medium 0.02% Tricaine and was maintained at 23°C throughout the imaging period.

Park O.K., Kwak J., Jung Y.J., Kim Y.H., Hong H.S., Hwang B.J., Kwon S.H, & Kee Y. (2015). 3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis. Molecules and Cells, 38(11), 975-981.

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Immunofluorescent Labeling of Acetylated Tubulin

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On day 7 of culture samples were fixed and permeabilized in 0.5% Triton-X 100 in PBS for 10 min. Blocking with 5% goat serum was performed for 1 h at room temperature before the primary antibody [anti-acetylated α-tubulin clone 6-11B-1 produced in mouse (1 μg/ml)] was applied for 24 h at 5°C. The secondary antibody [goat anti-mouse IgG H&L Alexa Fluor^® 488 (2 μg/ml, Abcam, United Kingdom)] was then applied for 1 h at room temperature followed by counterstaining with DAPI. Three PBS washes were performed between each of the previous steps. The samples were imaged using a Z.1 light sheet microscope (Zeiss). Samples were mounted in 0.8% agarose (vol/vol in deionized water) within glass capillaries (size 4, Zeiss). Two 10 × NA 0.2 illumination optics (Zeiss) were used to illuminate the samples in combination with a W plan-apochromat 20 × /1.0 objective (Zeiss). Samples were excited using a 405 nm (20 mW) and a 488 nm (50 mW) laser. Z-stacks were taken and a maximum projection image created using the accompanied ZEN software.

Bahmaee H., Owen R., Boyle L., Perrault C.M., Garcia-Granada A.A., Reilly G.C, & Claeyssens F. (2020). Design and Evaluation of an Osteogenesis-on-a-Chip Microfluidic Device Incorporating 3D Cell Culture. Frontiers in Bioengineering and Biotechnology, 8, 557111.

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Gastrulation Dynamics Captured by Lightsheet

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Gastrulation movements were analyzed by lightsheet microscopy in drl:eGFP (Video 1) and sox17:eGFP transgenic wild-type and toddler mutant embryos. Imaging was performed as outlined in Pauli et al., 2014 (link). In brief, embryos were allowed to develop at 28°C until early dome stage. Within a single experiment, 2–5 embryos (containing at least one embryo of each genotype) were mounted in 1% low-melting point agarose in 1x Danieau’s in glass capillaries (Zeiss, Oberkochen, Germany). Image acquisition was performed at a Lightsheet Z.1 microscope (Zeiss) at 26.5°C in a fish-water-filled imaging chamber. Time-lapse movies of up to five embryos were recorded in the multi-view mode at time-intervals between frames no larger than 180 s.
Timelapse acquisition settings for imaging on the Lightsheet Z.1 (Zeiss) (Single Plane Illumination System): 20x/1.0 water immersion objective, 0.5x zoom; 488 nm laser; Detection: Dual PCO.Edge second-generation sCMOS cameras, liquid cooled, 1920 × 1920 pixels,~70 fps; 16 bit images (1920 × 1920 pixels; 878.8 µm x 878.8 µm; pixel size 0.46 µm); Dual-side illumination with online dual fusion and pivot scan; z-stacks: 80–190 slices per embryo; 1.6–2.5 µm intervals.

Norris M.L., Pauli A., Gagnon J.A., Lord N.D., Rogers K.W., Mosimann C., Zon L.I, & Schier A.F. (2017). Toddler signaling regulates mesodermal cell migration downstream of Nodal signaling. eLife, 6, e22626.

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Imaging Zebrafish Embryo Neurodevelopment

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Tg(neurod1:EGFP)^ia50 zebrafish embryos at shield stage (6 hpf) were injected with vehicle (4 nanoliters of 10% DMSO in water) or with the maximal tolerated dose of GCP in vehicle (160 pmoles/embryo) as assessed by a series of preliminary experiments (data not shown). Then, embryos were first anesthetized using 0.16 mg/ml tricaine, embedded in 0.5% low melting agarose (Top Vision Low Melting Point Agarose, Thermo Fisher Scientific), and mounted on glass capillaries (Carl Zeiss, Germany). Images were acquired using Zeiss LightSheet microscope V1 supported by ZenPro software. EGFP acquisition was performed using 488–30 nm laser and 505–545 nm filter. Images from the same experiment were taken with the same laser intensity and exposure time to generate comparable images. After acquisition, 3D images were generated using Arivis software (Zeiss). 3D reconstructions were exported as a single snap with the same compression settings.

Guerra J., Belleri M., Paiardi G., Tobia C., Capoferri D., Corli M., Scalvini E., Ghirimoldi M., Manfredi M., Wade R.C., Presta M, & Mignani L. (2024). Impact of an irreversible β-galactosylceramidase inhibitor on the lipid profile of zebrafish embryos. Computational and Structural Biotechnology Journal, 23, 1397-1407.

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Scaffold Ingrowth Evaluation by Lightsheet Microscopy

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As a further measure of cell ingress, live/dead staining was performed on day 14 and assessed by lightsheet microscopy. Scaffolds were rinsed in PBS then stained in 2 μM calcein AM (live cells) in PBS for 30 min at room temperature. Scaffolds were then rinsed in PBS, submerged in 20 μg/mL propidium iodide (dead cells) in alpha-MEM for 5 min at room temperature, then rinsed twice in PBS. Scaffolds were then cut using a scalpel (one vertical and one horizontal) so that internal surfaces could be imaged to assess ingrowth.
To image through lightsheet microscopy (Z.1 lightsheet microscope, Zeiss), scaffold sections were mounted in 0.8 vol% agarose in diH₂O in glass capillaries (size 4, Zeiss). Two 10 × NA 0.2 illumination optics (Zeiss) were used to illuminate the samples in combination with a W plan-apochromat 20 ×/1.0 objective (Zeiss). The samples were excited using a 405 nm (20 mW) and a 488 nm (50 mW) laser. Z-stacks were taken and a maximum projection image created using the Bio-Formats plug-in for Fiji[49 (link)].

Owen R., Sherborne C., Evans R., Reilly G.C, & Claeyssens F. (2020). Combined Porogen Leaching and Emulsion Templating to produce Bone Tissue Engineering Scaffolds. International Journal of Bioprinting, 6(2), 265.

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