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6 protocols using 1 2 dichlorobenzene

1

Synthesis of Metal Chalcogenide Nanoparticles

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Sb(III)Cl3 (>99.95%), sulfur (99.98%), EHA (>99%), paraffin oil (d = 0.827–0.890 g/mL), OlAm (70%), and isopropyl alcohol (IPA, 99.5%) were obtained from Sigma-Aldrich. Hexane (>98%) was purchased from Alfa Aesar, chlorobenzene (>99%) from Merck KGaA, and 1,2-dichlorobenzene (>98%) from Fisher Scientific. All chemicals were used without further purification.
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2

Fabrication of Organic Solar Cells

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The glass substrates were purchased from SCHOTT AG. PDMS SYLGARD 184 elastomer kit was purchased from Dow Corning. P3HT and phenyl-C61-butyric acid methyl ester (PCBM) were purchased from Sigma-Aldrich and Fisher Scientific, respectively. All other reagents (agarose, fluorescein, hydrofluoric acid, calcium nitrate, H2SO4, H2O2, FeCl3, and 1,2-dichlorobenzene) and supplies were purchased from Fisher Scientific and were used as received unless specified in the text. Tri-decafluoro-1,1,2,2-tetrahydrooctyl)triethoxy-silane (DTTS) was obtained from Gelest Inc.
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3

Synthesis and Characterization of Iron Nanoparticles

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Iron tri(acetylacetonate) (2 mmol), 1,2-tetradecanediol (10 mmol), oleic acid (6 mmol), oleylamine (6 mmol), dibenzyl ether, citric acid, diethyl ether, 2-methoxyethylamine, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), thioglycolic acid, hydroxylamine, and phenanthroline were all purchased from Sigma-Aldrich (St. Louis, Missouri). 1,2-dichlorobenzene and N,N’-dimethylformamide were from Acros Organics (Morris Plains, New Jersey). NH2-PEG (550 Da, 2000 Da, 5000 Da, and 10000 Da) were all obtained from Laysan Bio, Inc. (Arab, Alabama). Slide-A-Lyzer G2 dialysis cassettes, chambered slides, bovine calf serum (BCS) were acquired from Thermo Scientific (Rockford, Illinois). Dulbecco’s Modified Eagle’s Medium (DMEM), Dulbecco’s Phosphate Buffer Saline (DPBS), penicillin, and streptomycin were purchased from Gibco Life Technologies, Inc. (NY, USA).
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4

Synthesis of Anthracene-based Dyes

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1,4-diaminoanthracene-9,10-dione (Sigma Aldrich, Burlington, MA, USA) and 1,4-dichloro-5,8-dihydroxyanthracene-9,10-dione (TCI, Tokyo, Japan) were used without purification. Quinoline (Acros Organics, Geel, Belgium) was carefully distilled in a vacuum. Anhydrous dimethoxyethane, dioxolane and diglyme, reagent grade N-methylpyrrolidone, 1,2-dichlorobenzene and diethylcarbonate were purchased from Acros Organics (Geel, Belgium).
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5

Isolation and Culture of HDMECs

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Calcein-acetoxymethyl ester (Sigma Aldrich), Chloroform HPLC (Biosolve BV, stabilized with amylene), Chloroform 99% (Chem-Lab, stabilized with amylene), Chloroform-d 99.6% atom%D (TCI Europe N.V.), Cryopreserved HUVEC (Lonza), 1,10-Decanediol 98% (Sigma Aldrich), 1,2-Dichlorobenzene 99% (Acros organics), 1,12-Dodecanediol 99% (Sigma Aldrich), Endothelial basal medium (Promocell), 1,7-Heptanediol >98% (TCI Europe N.V.), 1,6-Hexanediol 99% (Sigma Aldrich), HUVECs (Sigma Aldrich, pooled), Methanol >99.8% (ChemLab), 1,9-Nonadediol 98% (Sigma Aldrich), 1,8-Octanediol 98% (Sigma Aldrich), 1,5-Pentanediol (Fluka), Sodium hydroxide (Sigma Aldrich), SupplementMix (Promocell), Terephthaloyl chloride ≥99% (Sigma Aldrich), Triethylamine (Fischer Scientific), 1,11-Undecanediol 95% (Chemreagents) were used as received.
HDMECs were isolated with approval of the Medical Ethical Committee from cutaneous breast tissue (UZ Ghent, Belgium, EC2017/0710). The tissue was obtained by performing a subcutaneous mastectomy with 3 healthy Caucasian donors of 23, 29 and 31 years old with a mean body mass index of 24, 29, 25 kg/m 2 respectively, while the cells were isolated as described earlier. 26
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6

Analytical Standards for Environmental Monitoring

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(R,S)-atenolol (≥98%), beta-estradiol (≥98%), pyrene (99%), tamoxifen (≥99%), salbutamol (≥96%), hexafluorobenzene (99%), HPLC-grade chlorobenzene (99.9%), methanol (≥99.9%), dichloromethane (≥99.5%), and trimethoprim (≥99%), as well as the EPA 8279 organophosphorous pesticide mix 2 (CRM47908: dimethoate, disulfoton, famphur, parathion, parathion-methyl, phorate, sulfotep, thionazin, and triethyl thiophosphate) were purchased from Sigma-Aldrich (Buchs, Switzerland).
Benzothiazole (>96%) and N,N-diethyl-meta-toluamide (>98%) were obtained from Tokyo Chemical Industry (Eschborn, Germany), 1H-benzotriazole (99%) from ABCR Chemicals (Karlsruhe, Germany), caffeine (≥99%), fluorobenzene (>99.5%), and toluene (>99.7%) from Fluka-Chemie AG (Neu-Ulm, Switzerland), carbamazepine (99%), hexane (96%), 1,2-dichlorobenzene (>99%) and acetone for residue analysis (99.9%) from Acros Organics (Geel, Belgium), sulfamethoxazole from Apollo Scientific Ltd (Stockport, UK), and HPLC grade acetonitrile from Fisher Scientific (Reinach, Switzerland). Chromatography grade LiChrosolv® water was used and purchased from VWR International GmbH (Dietikon, Switzerland).
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