Chemicals were used as received without further purification. Dopamine hydrochloride and gelatin (from porcine skin Type A) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium alginate (80–120 mPa·s) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride
Manufactured by Tokyo Chemical Industry
Sourced in Japan
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is a water-soluble chemical compound commonly used as a coupling agent in organic synthesis. It is used to facilitate the formation of amide bonds between carboxylic acids and primary amines.
Automatically generated - may contain errors
Lab products found in correlation
L lactic acid, by Merck Group (2 mentions)
Trifluoroborane etherate, by Merck Group (2 mentions)
Glycolic acid, by Tokyo Chemical Industry (2 mentions)
Sodium alginate, by Fujifilm (1 mentions)
N hydroxysuccinimide nhs, by Tokyo Chemical Industry (1 mentions)
Type a gelatin from porcine skin, by Merck Group (1 mentions)
Dopamine hydrochloride, by Merck Group (1 mentions)
Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions)
Ix81 fluorescence microscope, by Olympus (1 mentions)
N acetylneuraminic acid, by Nacalai Tesque (1 mentions)
Bca protein assay kit, by Takara Bio (1 mentions)
4 dimethylaminopyridine, by Merck Group (1 mentions)
N n diisopropylcarbodiimide, by Merck Group (1 mentions)
Legato 101 syringe pump, by KD Scientific (1 mentions)
Succinic acid, by Tokyo Chemical Industry (1 mentions)
Anti twist, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
Mnso4 h2o, by Merck Group (1 mentions)
5 5 dimethyl 1 pyrroline n oxide, by Merck Group (1 mentions)
Anti upa antibody, by Santa Cruz Biotechnology (1 mentions)
8 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride
1
Dopamine-Loaded Alginate Hydrogel Synthesis
2
Carbodiimide-Activated Sialic Acid Functionalization
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The following materials were used: disodium iminodiacetate monohydrate (98%, WAKO Chemical Co., Ltd., Odawara, Japan), microfiber nonwoven fabric (polypropylene, dg 38~183%, ENEOS Corporation, Tokyo, Japan), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (98%, Tokyo chemical industry Co., Ltd., Tokyo, Japan), N-acetylneuraminic acid (Nacalai tesque, INC, Kyoto, Japan), WGA-Biotin (J220, J-chemical, INC, Tokyo, Japan), sodium dodecyl sulfate (95%, WAKO Chemical Co., Ltd., Odawara, Japan), BCA Protein Assay Kit (T9300A, Takara, Kusatsu, Japan), Synergy HT Multimode Microplate Readers (Biotek, Winooski, VT, USA), Spectrum Two FT-IR Spectrometer (Perkin Elmer, Waltham, MA, USA), wheat germ agglutinin, Triticum vulgaris, FITC Conjugate (FL-1021, Vector Laboratories, Burlingame, CA, USA), Olympus IX81 fluorescence microscope (Olympus Optical Co. Ltd., Tokyo, Japan), and FE-SEM-EDS (JSM-7800f, Jeol, Tokyo, Japan).
3
Flow Synthesis of Poly(glycolic-co-lactic acid)
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l -Lactic acid (≥98%), trifluoroborane etherate, Pd/C (10%), 4-(dimethylamino)pyridine, and N,N′-diisopropylcarbodiimide were purchased from Sigma-Aldrich and used without further purification. Glycolic acid (≥98%) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were purchased from Tokyo Chemical Industry and used without purification. Dichloromethane (DCM) and THF were distilled over CaH2 under N2. Legato 101 syringe pump was purchased from KD Scientific. Cadent 3 syringe pump was purchased from IMI Norgren. SEP-10 was purchased from Zaiput Flow Technologies. Perfluoroalkoxy tubing (1/16″ outside diameter/0.02″ and 0.03″ inside diameter) was purchased from Revodix. Omnifit EZ column was purchased from Diba Industries Inc. Gastorr AG-42-01 was purchased from GL Sciences. CF-2 fraction collector was purchased from Spectrum Chemical Mfg. Corp.
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4
Manganese-Catalyzed Oxidative Silica Nanoparticles
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Cetyltrimethylammonium chloride (CTAC, 25 wt%), tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTES), MnSO4·H2O, glucose oxidase, and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were obtained from Sigma-Aldrich. Triethanolamine (TEA) and methylene blue (MB) were obtained from Aladdin Industrial Inc. Disodium maleate, succinic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), and 1-hydroxybenzotriazole monohydrate (HOBT) were obtained from Tokyo Chemical Industry Co., Ltd. Polyethylene imine (PEI, molecular weight 5000, 50 wt%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Small interfering RNA against Twist (anti-Twist siRNA) (5′-GCU GAG CAA GAU UCA GAC CTT-3′ (sense)), FAM-labeled scrambled RNA (FAM siRNA), and Cy5-labeled scrambled RNA were obtained from Guangzhou Ribo Bio-Technology Co., Ltd. RNase A and RNase inhibitor were bought from the Beyotime Institute of Biotechnology. The anti-Twist, anti-beta actin, and anti-MMP-9 were purchased from Abcam Biotechnology. The anti-uPA antibody was obtained from Santa Cruz Biotechnology.
5
Synthesis of Chiral Organic Compounds
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l -Lactic acid (≥98%), l -mandelic acid (≥99%), allyl bromide, tert-butyldimethylsilyl chloride, trifluoroborane etherate, tetrakis(triphenylphosphine)palladium
(0), and morpholine were purchased from Sigma-Aldrich and used without
further purification. Glycolic acid (≥98%) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride were purchased from Tokyo Chemical Industry and used
without purification.d -Phenyl lactic acid (≥95%)
was purchased from AK Scientific, Inc. and used without purification.
Dichloromethane was distilled over CaH2 under N2.
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(0), and morpholine were purchased from Sigma-Aldrich and used without
further purification. Glycolic acid (≥98%) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride were purchased from Tokyo Chemical Industry and used
without purification.
was purchased from AK Scientific, Inc. and used without purification.
Dichloromethane was distilled over CaH2 under N2.
6
Synthesis and Characterization of Fluorescent Cyclodextrin Nanoparticles
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The following chemicals were used as received: mono-(6-amino-6-deoxy)-beta-cyclodextrin was bought from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd (Binzhou, China). Hyaluronic acid (HA, 95%), fluoresceinisothiocyanate (FITC, purity > 90%, Mw = 389.40), fluorescein sodium, phosphate buffer solution (PBS) and Arginylglycylaspartic acid(RGD) peptides-FITC (FITC-RGD & FITC-RGD-Ad) modified with fluoresceinisothiocyanate were obtained from Sinopharm Chemical Reagent Beijing Co., Ltd (Beijing, China). Cetyltrimethylammonium bromide surfactant (CH3(CH2)15N(CH3)3Br, CTAB), tetraethoxysilane (TEOS) and Poly(allylamine hydrochloride) (PAH, Mw = 15,000) were purchased from Sigma-Aldrich. 4,4′-diazostilbene-2,2′-disulfonic acid disodium salt (DAS), N-Hydroxysuccinimide (NHS) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Tokyo Chemical Industry (Shanghai) Development Co., Ltd (Shanghai, China). Only Ultra-pure water was used, in this study.
7
Synthesis of Amino Acid Derivatives
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Proteinogenic L-amino acids and their enantiomers, as well as γ-aminobutyric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). L-Tryptophan methyl ester hydrochloride and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and o-phthalaldehyde (OPA), Boc-L-cysteine, 5-oxo-D-proline, and 5-oxo-L-proline were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), Novabiochem (Läufelfingen, Switzerland), Combi-Blocks, Inc. (San Diego, CA, USA), and Enamin Ltd. (Kiev, Ukraine), respectively. All other chemicals were of the highest grade available and were purchased from commercial sources.
8
Synthesis of Multifunctional Hybrid Nanoparticles
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Poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) (PEO20PPO70PEO20: P123, Molecular weight (MW) = 5800), tetraethyl orthosilicate (TEOS, 98%), 3-aminopropyltriethoxysilane (APTES, ≥98%), N-hydroxysuccinimide (NHS, 98%), tetrahydrofuran (THF, 99.9%), toluene, cadmium nitrate tetrahydrate (CdN2O8·4H2O, ≥98%), cobalt(II) nitrate hexahydrate (Co(NO3)2·6H2O, ≥98%), cesium nitrate (CsNO3, 99%), zinc nitrate hexahydrate (Zn(NO3)2·6H2O, ≥99%), strontium nitrate (Sr(NO3)2, 99%), and lithium nitrate (LiNO3, 99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4′-Carboxybenzo-18-crown-6-ether (Crown, >97%) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 98%) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Hydrochloric acid and sodium hydroxide were purchased from DaeJung Chemicals & Metals Co., Ltd. (Siheung, Korea). Anhydrous ethanol (99%) was purchased from Junsei Chemical (Tokyo, Japan). Potassium dichromate (K2Cr2O7, ≥99%) was purchased from Osaka Chemical (Osaka, Japan). All reagents were used without further purification [23 (link)].
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