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Anti hdac1 hrp

Manufactured by Merck Group

Anti-HDAC1-HRP is a laboratory reagent used for the detection and quantification of HDAC1 (Histone Deacetylase 1) protein in various biological samples. It is a conjugate of an anti-HDAC1 antibody and horseradish peroxidase (HRP), enabling colorimetric or chemiluminescent detection of HDAC1 in assays such as ELISA, Western blotting, and immunohistochemistry.

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2 protocols using anti hdac1 hrp

1

Protein Interaction Analysis via Pulldown and Western Blot

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Pulldown assays and western blot analyses used for the MTAN-HDAC-MBDGATAD2CC subcomplex (in Figure S3D) and PWWP2A interaction studies (in Figure 5) were performed essentially as described in Torrado et al. (2017) (link). Briefly, after FLAG-affinity purification (as described above), protein samples were separated via SDS-PAGE using Bolt™ 4%–12% Bis-Tris Plus gels (Thermo Fisher Scientific) run in MES buffer at 165 V for 45 min. The gel-separated proteins were blotted onto PVDF membranes and probed with antibodies at the following concentrations: from Cell Signaling Technology: anti-HA-HRP (2999S, 1:40,000) and anti-HDAC1-HRP (59581S, 1:80,000); from Sigma-Aldrich: anti-FLAG-HRP (A8592, 1:80,000); from Abcam: anti-MBD3 (ab157464, 1:2,500); from Leinco Technologies: anti-rabbit-HRP (R115, 1:10,000). Blots were imaged using enhanced chemiluminescence and X-ray films.
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2

Protein Interaction Analysis via Pulldown and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pulldown assays and western blot analyses used for the MTAN-HDAC-MBDGATAD2CC subcomplex (in Figure S3D) and PWWP2A interaction studies (in Figure 5) were performed essentially as described in Torrado et al. (2017) (link). Briefly, after FLAG-affinity purification (as described above), protein samples were separated via SDS-PAGE using Bolt™ 4%–12% Bis-Tris Plus gels (Thermo Fisher Scientific) run in MES buffer at 165 V for 45 min. The gel-separated proteins were blotted onto PVDF membranes and probed with antibodies at the following concentrations: from Cell Signaling Technology: anti-HA-HRP (2999S, 1:40,000) and anti-HDAC1-HRP (59581S, 1:80,000); from Sigma-Aldrich: anti-FLAG-HRP (A8592, 1:80,000); from Abcam: anti-MBD3 (ab157464, 1:2,500); from Leinco Technologies: anti-rabbit-HRP (R115, 1:10,000). Blots were imaged using enhanced chemiluminescence and X-ray films.
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