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Rabbit anti dykddddk

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Rabbit anti-DYKDDDDK is a primary antibody that recognizes the DYKDDDDK peptide tag, also known as the FLAG tag. It is commonly used for the detection and purification of recombinant proteins that have been engineered to express this tag.

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Lab products found in correlation

Mouse anti flag m2, by Merck Group (3 mentions) Mouse anti acetylated α tubulin, by Merck Group (3 mentions) Alexa fluor plus 405 phalloidin, by Thermo Fisher Scientific (3 mentions) Phalloidin 650, by Thermo Fisher Scientific (3 mentions) Fluoro gel, by Electron Microscopy Sciences (3 mentions) Rabbit anti rnaseh2c, by Proteintech (2 mentions) Rabbit anti rnaseh2a, by OriGene (2 mentions) Mouse anti rnaseh2a, by Abcam (2 mentions) Mouse anti γh2ax jbw301, by Merck Group (2 mentions) Rabbit anti rad51 h 92, by Santa Cruz Biotechnology (2 mentions) Rabbit anti parp1 h 250, by Santa Cruz Biotechnology (2 mentions) Mouse anti par 10h, by Enzo Life Sciences (2 mentions) Rabbit anti topoisomerase 1, by Abcam (2 mentions) Mouse anti α tubulin dm1a, by Merck Group (2 mentions) Rabbit anti dna polymerase beta, by Abcam (2 mentions) Mouse anti rnaseh2a g 10, by Santa Cruz Biotechnology (2 mentions) Mouse anti α tubulin b512, by Merck Group (2 mentions) Mouse anti cas9, by Diagenode (2 mentions) Mouse anti vinculin, by Merck Group (2 mentions) Rabbit anti actin, by Merck Group (2 mentions)

5 protocols using rabbit anti dykddddk

1

Immunofluorescence Staining of Embryos

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For antibody staining, embryos were fixed with 3% PFA in PBS, blocked in 10% goat serum, and primary and secondary antibody solutions were prepared in 5% goat serum. Mouse anti-acetylated α-tubulin (T7451; Sigma-Aldrich) was used at a 1:500 dilution, mouse anti-beta tubulin (DHSB; E7) supernatant was used at a 1:10 dilution. Mouse anti-FLAG M2 (F3165; Sigma) and rabbit anti-DYKDDDDK (2368; Cell Signaling) were used at 1:100 dilutions. E7 (anti-tubulin) was deposited to the DSHB by Klymkowsky, M (Chu and Klymkowsky, 1989 (link)). Cy-2–, Cy-3–, or Cy-5–conjugated goat anti-mouse secondary antibodies were used at the manufacturers’ recommended dilution. Phalloidin 650 (1:600, Invitrogen) and Alexa Fluor Plus 405 Phalloidin (1:100, Invitrogen) were used to visualize actin. Embryos were mounted between two coverslips using Fluoro-Gel (Electron Microscopy Sciences).
Collins C., Kim S.K., Ventrella R., Carruzzo H.M., Wortman J.C., Han H., Suva E.E., Mitchell J.W., Yu C.C, & Mitchell B.J. (2021). Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell reports, 36(7), 109556.
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2

Antibody Characterization for Protein Detection

Cited 1 time
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The following antibodies were used for immunofluorescence (IF) and immunoblotting (IB) at indicated dilutions: sheep anti-pan-RNase H2 (raised against human recombinant RNase H213 (link), IB 1:1,000, IP 5 µl / 1 ml lysate); rabbit anti-RNASEH2C (Proteintech 16518-1-AP; IB 1:1,000); rabbit anti-RNASEH2A (Origene TA306706, IB 1:1,000); mouse anti-RNASEH2A (Abcam ab92876; IF 1:500); mouse anti-RNASEH2A G-10 (Santa Cruz Biotechnologies sc-515475; WB 1:1000); mouse anti-γH2AX JBW301 (Millipore 05-636, IF 1:800 – 1:2,000); rabbit anti-RAD51 H-92 (Santa Cruz Biotechnologies sc-8349, IF 1:150); rabbit anti-BRCA146 (link) (IB 1:1,000); mouse anti-Cas9 (Diagenode C15200203, IB 1:1,000); rabbit anti-PARP1 H-250 (Santa Cruz Biotechnologies sc-7150, IB 1:1,000); mouse anti-PAR 10H (Enzo ALX-804-220-R100, IB 1:1,000); rabbit anti-Topoisomerase I (Abcam ab109374; IB 1:5,000); rabbit anti-DYKDDDDK (Cell Signaling Technologies 2368, IB 1:1,000); rabbit anti-actin (Sigma A2066, IB 1:5,000); mouse anti-α–tubulin DM1A (Millipore CP06, IB 1:5,000); mouse anti-α–tubulin B512 (Sigma T6074, IB 1:5,000); rabbit anti-GAPDH (Sigma G9545, IB 1:20,000); mouse anti-vinculin (Sigma V9264, IB 1:1,000); rabbit anti-DNA polymerase beta (Abcam ab26343, IB 1:1,000); rabbit anti-cleaved caspase-3 (Cell Signaling Technologies 9661S, IF 1:800).
Zimmermann M., Murina O., Reijns M.A., Agathanggelou A., Challis R., Tarnauskaitė Ž., Muir M., Fluteau A., Aregger M., McEwan A., Yuan W., Clarke M., Lambros M.B., Paneesha S., Moss P., Chandrashekhar M., Angers S., Moffat J., Brunton V.G., Hart T., de Bono J., Stankovic T., Jackson A.P, & Durocher D. (2018). CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature, 559(7713), 285-289.
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3

Immunofluorescence Staining of Embryos

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For antibody staining, embryos were fixed with 3% PFA in PBS, blocked in 10% goat serum, and primary and secondary antibody solutions were prepared in 5% goat serum. Mouse anti-acetylated α-tubulin (T7451; Sigma-Aldrich) was used at a 1:500 dilution, mouse anti-beta tubulin (DHSB; E7) supernatant was used at a 1:10 dilution. Mouse anti-FLAG M2 (F3165; Sigma) and rabbit anti-DYKDDDDK (2368; Cell Signaling) were used at 1:100 dilutions. E7 (anti-tubulin) was deposited to the DSHB by Klymkowsky, M (Chu and Klymkowsky, 1989 (link)). Cy-2–, Cy-3–, or Cy-5–conjugated goat anti-mouse secondary antibodies were used at the manufacturers’ recommended dilution. Phalloidin 650 (1:600, Invitrogen) and Alexa Fluor Plus 405 Phalloidin (1:100, Invitrogen) were used to visualize actin. Embryos were mounted between two coverslips using Fluoro-Gel (Electron Microscopy Sciences).
Collins C., Kim S.K., Ventrella R., Carruzzo H.M., Wortman J.C., Han H., Suva E.E., Mitchell J.W., Yu C.C, & Mitchell B.J. (2021). Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell reports, 36(7), 109556.
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4

Antibody Characterization for Protein Detection

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunofluorescence (IF) and immunoblotting (IB) at indicated dilutions: sheep anti-pan-RNase H2 (raised against human recombinant RNase H213 (link), IB 1:1,000, IP 5 µl / 1 ml lysate); rabbit anti-RNASEH2C (Proteintech 16518-1-AP; IB 1:1,000); rabbit anti-RNASEH2A (Origene TA306706, IB 1:1,000); mouse anti-RNASEH2A (Abcam ab92876; IF 1:500); mouse anti-RNASEH2A G-10 (Santa Cruz Biotechnologies sc-515475; WB 1:1000); mouse anti-γH2AX JBW301 (Millipore 05-636, IF 1:800 – 1:2,000); rabbit anti-RAD51 H-92 (Santa Cruz Biotechnologies sc-8349, IF 1:150); rabbit anti-BRCA146 (link) (IB 1:1,000); mouse anti-Cas9 (Diagenode C15200203, IB 1:1,000); rabbit anti-PARP1 H-250 (Santa Cruz Biotechnologies sc-7150, IB 1:1,000); mouse anti-PAR 10H (Enzo ALX-804-220-R100, IB 1:1,000); rabbit anti-Topoisomerase I (Abcam ab109374; IB 1:5,000); rabbit anti-DYKDDDDK (Cell Signaling Technologies 2368, IB 1:1,000); rabbit anti-actin (Sigma A2066, IB 1:5,000); mouse anti-α–tubulin DM1A (Millipore CP06, IB 1:5,000); mouse anti-α–tubulin B512 (Sigma T6074, IB 1:5,000); rabbit anti-GAPDH (Sigma G9545, IB 1:20,000); mouse anti-vinculin (Sigma V9264, IB 1:1,000); rabbit anti-DNA polymerase beta (Abcam ab26343, IB 1:1,000); rabbit anti-cleaved caspase-3 (Cell Signaling Technologies 9661S, IF 1:800).
Zimmermann M., Murina O., Reijns M.A., Agathanggelou A., Challis R., Tarnauskaitė Ž., Muir M., Fluteau A., Aregger M., McEwan A., Yuan W., Clarke M., Lambros M.B., Paneesha S., Moss P., Chandrashekhar M., Angers S., Moffat J., Brunton V.G., Hart T., de Bono J., Stankovic T., Jackson A.P, & Durocher D. (2018). CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature, 559(7713), 285-289.
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5

Antibody Staining of Embryos

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For antibody staining, embryos were fixed with 3% PFA in PBS, blocked in 10% goat serum, and primary and secondary antibody solutions were prepared in 5% goat serum. Mouse anti-acetylated α-tubulin (T7451; Sigma-Aldrich) was used at a 1:500 dilution, mouse anti-beta tubulin (DHSB; E7) supernatant was used at a 1:10 dilution. Mouse anti-FLAG M2 (F3165; Sigma) and rabbit anti-DYKDDDDK (2368; Cell Signaling) were used at 1:100 dilutions. E7 (anti-tubulin) was deposited to the DSHB by Klymkowsky, M (Chu and Klymkowsky, 1989) .. Cy-2-, Cy-3-, or Cy-5-conjugated goat anti-mouse secondary antibodies were used at the manufacturers' recommended dilution. Phalloidin 650 (1:600, Invitrogen) and Alexa Fluor Plus 405 Phalloidin (1:100, Invitrogen) were used to visualize actin. Embryos were mounted between two coverslips using Fluoro-Gel (Electron Microscopy Sciences).
Collins C., Kim S.K., Ventrella R., Mitchell J., & Mitchell B.J. (2021). Tubulin Acetylation Promotes Penetrative Capacity of Cells Undergoing Radial Intercalation.
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