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2 protocols using l α lecithin

1

Soybean Lecithin and Cholesterol Protocol

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L-α-Lecithin, (L-α-phosphatidyl choline, PC), from soybean oil and cholesterol, 99 %, (CH) were purchased from Acros organics, Belgium. Diethylnitrosamine (DEN) was purchased from Sigma-Aldrich Co (Steinheim, Germany). Carbon tetrachloride (CCl4) was obtained from El-Gomhorya Company, Cairo, Egypt. Kits used for the determination of liver function and oxidative stress biomarkers (MDA, GST and GSH) were obtained from Randox Company (Antrim-UK). Primers specific for PI3K, AKT, PTEN, and STAT-5A that were used in real time-PCR were purchased from Qiagen (Germany-Helden). All other chemicals are of high analytical grade.
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2

Delamanid Formulation Development and Characterization

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Delamanid (DLM; 99.9%)
was purchased from Gojira Fine Chemicals, LLC (Bedford Heights, OH).
Hydroxypropyl methylcellulose (HPMC; Pharmacoat 603), hydroxypropyl
methylcellulose phthalate (HPMCP; HP-50), and hydroxypropyl methylcellulose
acetate succinate (HPMCAS; AQOAT AS-LF and AS-HF) were generously
provided by Shin-Etsu Chemical Co. (Tokyo, Japan). Dichloromethane
(DCM; HPLC Plus grade) and Tween 80 were purchased from Sigma-Aldrich
(St. Louis, MO). Polycaprolactone diol (PCL; MW 1250 Da) was purchased
from Polysciences, Inc. (Warrington, PA). l-α-Lecithin,
anisole (99%), and methyl tert-butyl ether (MTBE;
99.9%) were purchased from Acros Organics (Geel, Belgium). Tetrahydrofuran
(THF; HPLC grade), acetonitrile (HPLC grade), methanol (HPLC grade),
chloroform (HPLC grade), ethyl acetate (ACS grade, 99.9%), sodium
hydroxide, and trifluoroacetic acid were purchased from Fisher Scientific
(Hampton, NH). N-butyl acetate (>99%) was purchased from Alfa Aesar
(Ward Hill, MA). HEPES (4-[2-Hydroxyethyl]-1-piperazine ethanesulfonic
acid) was purchased from Boehringer Mannheim (Mannheim, Germany).
Deionized (DI) water (18.2 MΩ cm) was prepared by a Barnstead
Nanopure Diamond ultrapure water system (Thermo Scientific, Waltham,
MA).
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