The human breast cancer cell lines MCF7, T47D, ZR-75-1 (ER+/HER2−), BT474 (ER+/HER2+), SKBR3 and JIMT-1 (ER−/HER2+) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The MCF7, T47D, ZR-75-1, SKBR3 and JIMT-1 cell lines were cultured in an RPMI 1640 medium (Sigma, St. Louis, MO, USA), while the BT474 cell line was cultured in a DMEM medium (Sigma). All culture media were supplemented with 10% fetal bovine serum (FBS) (Sigma), an antibiotic-antimycotic solution (1×) (Sigma), and l -glutamine (2 mM) (Invitrogen GmbH, Karslruhe, Germany). The cultures were maintained in an incubator at 37 °C under 5% CO2.
Jimt 1
Manufactured by Merck Group
Sourced in United States
JIMT-1 is a cell line derived from a human breast carcinoma. It is commonly used in cancer research as a model system for studying breast cancer biology and evaluating potential therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
Sk br 3, by Merck Group (3 mentions)
Bt474, by Merck Group (3 mentions)
Skbr3, by American Type Culture Collection (3 mentions)
Jimt 1, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Zr 75 1, by Merck Group (2 mentions)
Mcf 7, by Merck Group (2 mentions)
Dmem medium, by Merck Group (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Zr 75 1, by American Type Culture Collection (2 mentions)
Mda mb 361, by Merck Group (2 mentions)
Bt474, by American Type Culture Collection (2 mentions)
Mda mb 361, by American Type Culture Collection (2 mentions)
Antibiotic antimycotic solution 1, by Merck Group (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
4 protocols using jimt 1
1
Culturing Breast Cancer Cell Lines
2
Breast Cancer Cell Line Characterization
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Nine established breast cancer cell lines [MCF7, T47D, ZR-75-1 (estrogen receptor positive (ER+), HER2 not amplified), BT474, MDA-MB361 (ER+, HER2 amplified), SKBR3, JIMT-1 and KPL4 (ER-, HER2 amplified) and MDA-MB231 (ER-, HER2 not amplified)] were obtained from the American Type Culture Collection (ATCC, Manassas, USA). The MCF7, T47D, ZR-75-1, SKBR3, JIMT-1 and KPL4 cell lines were cultured in RPMI 1640 medium (Sigma, St. Louis, MO, USA), while the BT474, MDA-MB231 and MDA-MB361 lines were cultured in DMEM medium (Sigma). All culture media were supplemented with 10% fetal bovine serum (FBS) (Sigma), an antibiotic-antimycotic solution (1X) (Sigma) and L-glutamine (2 mM) (Invitrogen GmbH, Karslruhe, Germany). The cultures were maintained in an incubator at 37°C and 5% CO2.
3
Prostate Cancer Cell Line Culture
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RWPE1, DU145, PC3, 22Rv1, LNCaP, VCaP and JIMT-1 cell lines were purchased from ATCC. LNCaP 1F5 and V16A were a gift from Dr. Olli Jänne from the University of Helsinki. RWPE1 cells were cultured in Keratinocyte SFM medium (Gibco) supplemented with bovine pituitary extract and human recombinant EGF, and standard antibiotics: penicillin (100 units/ml) and streptomycin (100 μg/ml), according to the manufacturer’s protocol. PC3 and DU145 cells were maintained in F12K (Gibco) and MEM (Gibco) medium, respectively containing 10% fetal bovine serum (Gibco) and standard antibiotics. 22Rv1, VCap, LNCaP, LNCaP 1F5, V16A and JIMT-1 cells were grown in RPMI1640 (Sigma-Aldrich) containing 10% FBS and standard antibiotics. The cells were maintained at 37 °C in a humidified atmosphere with 5% CO2. All cell lines were confirmed to be mycoplasma-free during the analysis.
4
Establishment of Trastuzumab-Resistant Breast Cancer Cell Lines
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The SKBR3, BT474, and MDA-MB-361 cell lines were purchased from the American Type Culture Collection. The JIMT-1 cell line was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen. All cell lines were derived from HER2+ breast cancer cells. The SKBR3 cell line was maintained in McCoy’s 5A medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum. The BT474, MDA-MB-361, and JIMT-1 cell lines were maintained in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum. Human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell (Heidelberg, Germany) and were maintained in Endothelial Cell Growth Medium 2 (PromoCell). For generation of Tzm-resistant cell lines, SKBR3 and BT474 cells were grown in DMEM/F12 supplemented with 5% calf serum (Sigma-Aldrich), 4 μg/mL insulin (Thermo Fisher Scientific), 0.5 μg/mL hydrocortisone (Stem Cell Technologies, Vancouver, Canada), and 1 μg/mL (for SKBR3) or 2 μg/mL (for BT474) Tzm (Herceptin; Chugai Pharmaceutical, Tokyo, Japan) for over 6 months.
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